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Austral journal of veterinary sciences

versión impresa ISSN 0719-8000versión On-line ISSN 0719-8132

Austral j. vet. sci. vol.54 no.2 Valdivia  2022 


Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Ana Umpiérreza  * 

Déborah Ernsta 

Andrea Cardozoa 

Alexia Torresb 

Magalí Fernándeza 

Martín Fragac 

Rafael Vignolid 

Inés Badod 

Roberto Vidalb  e 

Pablo Zuninoa 

aDepartamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable,. Avenida Italia 3318. CP: 11600. Montevideo, Uruguay.

bPrograma de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.

cPlataforma de Investigación en Salud Animal. Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA La Estanzuela. Ruta 50, Km 11. CP: 70000. Colonia, Uruguay.

dDepartamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República. Avenida Alfredo Navarro 3051. CP: 11600. Montevideo, Uruguay.

eInstituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile.


Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and II and phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c, stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2a were detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics.

Key words: Non-O157 STEC; Shiga toxin subtypes; antimicrobial resistance


Shiga toxin-producing Escherichia coli (STEC) is a bacterial pathogen with a defined zoonotic potential (Gyles & Fairbrother, 2010). The main natural reservoir of STEC is the bovine intestine, although it can be isolated from other domestic animals (Gyles & Fairbrother, 2010). Infections in humans are usually caused by the consumption of undercooked meat, contaminated vegetables, dairy products and contact with contaminated water. Some infections are also caused by the contact with the environment of animals and ruminants in the farm and person-to-person contact (Kintz et al., 2017). Even though some individuals infected with STEC recover without significant complications, some STEC strains are highly virulent to humans (Majowicz et al., 2014). STEC infections cause over 2.8 million illnesses annually, including haemorrhagic colitis (HC), haemolytic uraemic syndrome (HUS), renal failure and even in some cases haemorrhagic cystitis around the world (Gadea et al., 2012, Majowicz et al., 2014). The incidence of STEC infections differs between countries alongside South America. In Uruguay, cases of HUS and HC are sporadic and have an incidence of 4 to 5 per 100,000 children, whereas Argentina, considered the country with the highest incidence of HUS in children under 5 years, has 300 to 400 HUS cases per year (Blanco et al., 2004, Pérez et al., 2014).

STEC main virulence factors are Shiga toxin type 1 and type 2 (Stx1 and Stx2, respectively). They are encoded in the genome of tempered double-stranded lambdoid prophages (Scheutz, 2014). The number of Stx1 and Stx2 subtypes is continuously upgrading. The Stx1 group is conserved and has four subtypes, a, c, d, and e, while Stx2 is more heterogeneous, and 11 subtypes have been distinguished so far: a-k, with some of them been reported in severe disease in humans (Probert et al., 2014, Scheutz, 2014, Bai et al., 2018, Yang et al., 2020a). In addition, some STEC isolates possess the locus of enterocyte effacement (LEE) pathogenicity island. STEC LEE+ strains are defined by the expression of Intimin, and the translocated intimin receptor (Tir), among other virulence factors. Together, they are responsible for the attaching and effacing (A/E) lesions induced in intestinal epithelial cells (Torres et al., 2018).

Despite the great diversity of phenotypes and combinations of virulence factors, E. coli presents a clonal structure. So far, commensal E. coli strains, diarrheagenic E. coli from the diarrheagenic E. coli group (DEC), and those causing extraintestinal infections (ExPEC) can be grouped into eight phylogenetic groups: A, B1, B2, C, D, E, F, and clade I (Clermont et al., 2013). ExPEC strains have been mainly assigned to group B2 and a lesser extent to groups D and F, while commensals and diarrheagenic E. coli strains have been assigned mostly to groups A, B1, and E (Tenaillon et al., 2010). At the same time, E. coli strains are distinguished by their lipopolysaccharide (O) and flagellum (H) composition and antigenicity (Rivas et al., 2014). Currently, in addition to E. coli O157:H7, other serogroups have been associated with severe HUS outbreaks. Big six serotypes O26, O45, O103, O111, O121, and O145, together with E. coli O157, are the most prevalent within STEC LEE+ strains (Rivas et al., 2014).

As aforementioned, bovines are the main reservoir of STEC and besides the severe damage caused to humans, it can survive imperceptibly in both the bovine intestinal epithelium and in the environment. STEC abundance is low in cattle intestine, however, it can manage to be viably transmitted to the farm environment and from there, even in low abundances, to infect other animals or contaminate water courses (Sapountzis et al., 2020). Persistence in such different places is associated with the phenotypes and plasticity to adapt to the ecological niches and usually involves the ability to form biofilms or have thigh adhesion conditions. Autoaggregation, cell-cell adhesion to the host and biofilm formation of STEC have been associated with the presence of autotransporter proteins like Antigen 43 (Ag43). This adhesin has been more frequently linked to pathogenic STEC LEE+ strains like E. coli O157:H7 than to commensals ones (Kjaergaard et al., 2000).

The emergence of multidrug-resistant bacteria has been recognised as a global health issue. Antimicrobial misuse in humans and animals over the decades has determined the occurrence of non-effective treatments for several infectious diseases (Marshall & Levy, 2011). Even antibiotic treatment in STEC infections is not recommended, it is important to keep in mind the association of resistance mechanisms to mobile genetic elements, such as transposons, integrons and plasmids, that give bacteria the capability to rapidly transfer resistance genes (Marshall & Levy, 2011).

In Uruguay, the proportion of STEC in the faeces of calves is low, according to previous studies of our group (Umpiérrez et al., 2017, 2021). However, given the severity and outcome of the illness, the regional context of HUS cases in children, and the ecology of STEC transmission and persistence in the environment it is required to find out the potentially harmful effects of circulating STEC isolates. This study aimed to determine the Shiga toxin subtypes and the presence of the Antigen 43 gene, the antimicrobial phenotype, O-serogrouping, phylotypes and phylogenetic relatedness of STEC isolates of calf origin.



Sixteen STEC strains isolated from bovine faeces were collected between 2014 and 2017. As previously described, faeces collection and biochemical and molecular characterisation of E. coli were performed (Umpiérrez et al., 2017, 2021). Briefly, all isolates except one were collected from faeces of seven dairy calves with signs of neonatal calf diarrhoea (NCD) and from faeces of dairy calves without NCD signs under 35 days old (table 1), whereas one isolate was collected from an ileum sample of a dead calf affected with NCD (table 1). All isolates were previously characterised by PCR, regarding the presence of stx1, stx2, and eae among other E. coli virulence genes, and classified as STEC LEE+ (stx+/eae+) (Umpiérrez et al., 2017, 2021). For the routine cultivation of STEC, isolates were grown on trypticase soy agar (TSA) plates (OXOID) for 18-24 h at 37 ºC.


Subtyping of Stx was performed according to the protocol described by Scheutz et al., (2012). Three stx1subtypes (stx1a, stx1c, stx1d) and 7 stx2 subtypes (stx2a, stx2b, stx2c, stx2d, stx2e, stx2f, stx2g) were evaluated by multiplex PCR. When required, the whole stx operon was amplified and sequencing analysis was performed using the free software BioEdit (version7.2.5) to assign the Stx subtype to each isolate (Scheutz et al., 2012).


The presence of Ag43 was evaluated by PCR. Partial amplification of the ag43 gene was performed as previously described (Kjaergaard et al., 2000). Amplicon size of the two Ag43 subfamilies was determined by agarose gel electrophoresis (subfamily I amplicon size: 1569pb; subfamily II amplicon size: 1839pb) (Kjaergaard et al., 2000).


The O26, O45, O103, O111, O113, O121, O145, and O157 serogroups were determined by PCR according to the procedure described by Paddock et al., (2012). PCR positive controls were included in each amplified serogroup.

E. coli O157:H7 EDL933 strain was used as a positive control for O157 serogroup. The rest of the DNA controls were extracted from clinical isolates from the “Pathogenic Escherichia coli Laboratory” pathogenic strains collection at Universidad de Chile.


To assign STEC isolates into any of the phylogroups, partial amplifications of chuA, yjaA, tspE4.C2 and arpA genes by PCR was performed according to Clermont et al., (2013). The multiplex PCR method uses the amplification profile of these genes to assign isolates to eight different phylogroups: A, B1, B2, C, D, E, F, and Clade-I.

Table 1 Virulence profile, antimicrobial phenotype, and resistance genes, O-serogrouping and phylotypes of STEC isolates recovered from animals with signs of NCD and from healthy calves. 

Animal Isolate Yea isola r of Animal signs tion Virulence profile Antibiotic resistance profil Resistance Serogroup es genes Phylogroup
1 1 (74.2) 2014 NCD signs stx1a, ag43(II)--- O111 B1
2 2 (16.16) 2015 NCD signs(n) stx1a+cAMP / CN / FO TfosA7n/d A
3 3 (AG2.1) 2016 NCD signs stx2aFOT n/d A
4 4(AD1.5) 2016 NCD signs stx1a+c, ag43(I)AMP/CIP n/d E
5 (AD1.6) stx1a, ag43(I)AMP/CIP O111 F
6 (AD1.7) stx1a+cAMP n/d B1
7 (AD1.9) stx1a+cAMP O111 E
5 8 (AD3.2) 2016 NCD signs stx1a+c, ag43(II)AMP O111 B1
6 9 (AD7.2) 2016 NCD signs stx1aAMP O111 B1
10 (AD7.5) stx1aAMP O111 C
11 (AD7.10) stx1a, ag43(II)--- n/d E
7 12 (AC3.1) 2016 Calf without signs stx1a+c, ag43(II)CIP O103 B1
13 (AC3.10) stx1a, ag43(II)--- O103 B1
8 14 (BJ1.3) 2017 NCD signs stx1a/stx2e, ag43(II)--- O103 E
15 (BJ1.5) stx1a+c, stx2e, ag43(II)AMP O103 B1
16 (BJ1.10) stx1a, stx2e, ag43(II)AMP O103 B1

(n) E. coli isolated from an ileum sample of a dead calf with NCD. n/d: none determined serogroup. ag43(I) corresponds to Ag43 subfamily I, and ag43(II) corresponds to Ag43 subfamily II. AMP, Ampicillin; FOT, fosfomycin-trometamol; CN, gentamicin.


Clonality of the isolates was evaluated by XbaI Pulsed-Field Gel Electrophoresis (PFGE) according to PulseNet protocol. Salmonella Braenderup H9812 and Staphylococcus aureus subsp. aureus (strain NCTC 8325) were used as reference strains. Band patterns were analysed with BioNumerics v.6.6 software (Applied Maths, Sint- Martens-Latem, Belgium). A dendrogram was generated by the UPGMA method, using the Dice coefficient with a 1.0% of band position tolerance.


The Kirby-Bauer disc-diffusion method was used to analyse antimicrobial susceptibility, according to the Clinical Laboratory Standard Institute (2017). Grown isolates in Mueller-Hinton (MH) agar plates (OXOID) for 18-24 h at 37 ºC were tested for 11 different antibiotics: ampicillin (AMP), cefuroxime (CXM), ceftazidime (CAZ), ceftriaxone (CRO), nalidixic acid (NA), ciprofloxacin CIP, enrofloxacin (ENR), gentamicin (CN), amikacin (AK), trimethoprim-sulfamethoxazole (SXT), and fosfomycin trometamol (FOT). All antibiotic discs were purchased from Oxoid. Quality control was performed with E. coli ATCC 25922. The interpretation of results was performed according to CLSI 2017, except for ENR, which was interpreted using Veterinary Antimicrobial Susceptibility Testing (VAST) (Patel, 2014). Considering the One Health concept and the zoonotic potential of these isolates, all antibiotics were selected based on the frequency with which they are employed in the medical practice of human infectious diseases.


With regard to resistance genes, we searched for the main mechanisms of transferable resistance to antibiotics considered critical by the World Health Organization and previously detected in our country. The presence of PMQR and fosfomycin genes was evaluated by PCR. PMQR genes qnrA, qnrB, qnrC, qnrD, qnrS, and qepA were partially amplified as previously described (Umpiérrez et al., 2017). On the other hand, the search for fosfomycin resistance genes fosA, fosB, fomA, fomB, fosA3, fosC2, and fosA7 was performed following protocols and using primers from literature (Cóppola et al., 2020). Primers to detect fosA7 gene were designed in this study: fosA7F 5'- ATGCTTCAATCTCTGAACCAC -3', fosA7R 5'- CCGAAACGCATTCCAGAGTA -3'. All PCR products were confirmed by direct sequencing.


A collection of 16 STEC isolates (13 isolates from calves with NCD signs, two isolates from a calf without signs of NCD and one isolate from an ileum sample of a dead calf with NCD) were characterised.


Four Shiga toxins subtypes were detected by PCR. Some STEC isolates presented more than one variant of subtypes simultaneously. All stx1+ isolates were typed as stx1a+ (15 isolates), 6 of them were also stx1c+ and one presented a stx1a+/stx1c+/stx2e+ genotype (table 1). In addition, 2 isolates were stx1a+/stx2e+ simultaneously and 1 isolate was stx2a+ (table 1). Gene variants stx1d, stx2b, stx2c, stx2d, stx2f and stx2g were not detected.


Ag43 subfamily II, was predominant among STEC isolates (n=8), while only 2 isolates were assigned to Ag43 subfamily I (table 1). The rest of the isolates (n=6) were ag43 negative (table 1).


Two E. coli serogroups were detected by PCR. Five STEC isolates were ascribed to O103 serogroup, whereas 6 isolates were ascribed to O111 (table 1). The remaining 5 isolates were not assigned to any of the 8 serogroups evaluated (31.3% of the isolates) (table 1).

On the other hand, STEC phylotyping showed high diversity. Fifty per cent of the isolates were classified as B1 (n=8) (table 1), and the rest were classified as follows: 4 belonged to E phylogroup, 2 to A phylogroup, 1 to F phylogroup and one to C phylogroup (table 1).


A total of 13 distinct restriction patterns of the 16 STEC isolates were detected using ≥85% of similarity of the Dice coefficient (figure 1). A high level of diversity amongst isolates was observed, however, isolates with the same herd-origin were more genetically similar to each other and as a consequence showed more similar restriction patterns. Two isolates from 1 animal with symptoms of NCD (isolates AD1.5 and AD1.6) were considered indistinguishable from each other (figure 1). STEC isolates AC3.1 and AC3.10 from an asymptomatic animal were considered indistinguishable from each other (figure 1). Additionally, isolates AD1.7 and AD 7.2 coming from different animals but from the same farm, were considered identical (figure 1).


STEC isolates were mostly susceptible to antibiotics. Ten out of 16 (62.5%) of the isolates were resistant to AMP, whereas 4/16 (25%) were susceptible to all antibiotics tested. Ten STEC isolates were resistant to beta-lactams (10 isolates were resistant to ampicillin), 3 STEC isolates were resistant to ciprofloxacin and 2 STEC isolates were resistant to fosfomycin trometamol (table 1). The isolate from the ileum sample was the only one resistant to 3 antibiotics: ampicillin, gentamicin and fosfomycin trometamol, whereas 2 STEC isolates were ciprofloxacin- ampicillin-resistant (table 1).

PMQR genes were evaluated in 3 isolates that showed resistance or intermediate susceptibility to CIP: AD1.5, AD1.6 and AC3.1 (table 1). None of the 7 PMQR genes was detected. Otherwise, FOT gene fosA7 was detected in 1 STEC isolate: 16.16 (table 1).


STEC infections in humans constitute a global health concern and are endemic in Latin America, accounting for almost 2% of acute diarrhoea cases and 20%-30% of bloody diarrhoea (Torres et al., 2018). STEC colonises the gastrointestinal tract of cattle, which are mainly asymptomatic carriers, and only under specific circumstances can develop diarrhoea. It can adapt and survive in different environments such as soil and water and contaminates food, meat, and dairy products, through which it can reach and infect other animals and humans (Daly & Hill, 2016). Non-O157 STEC isolates have increasingly been reported associated with human outbreaks (Bettelheim & Goldwater, 2014).

The severity of STEC infections is determined by the interaction of both host and microorganism factors. Regarding the bacterial virulence profile, all detected Stx subtypes in the present study are linked to illness in humans (EFSA BIOHAZ Panel, 2020). We detected stx1a+c (n=8) and stx1a (n=6) genotypes in high proportion amongst isolates, which were similar to previous reports in non-O157:H7 STEC isolates from cattle faeces in the United States (Shridhar et al., 2017) and the occurrence of stx1a together with stx2a, stx2c, or stx2d have also been described in STEC isolates from bovine origin in China (Fan et al., 2019). In this study, stx1a/stx2e and stx1a+c/ stx2e genotypes were detected. Stx2e is associated with mild gastroenteritis in humans, while it is the most frequent Stx subtype in pigs, wild boars, and their meat products (Beutin et al., 2008). Additionally, stx2a gene variant, widely associated with HUS, was detected in one isolate from an animal with signs of NCD. This is the first report of the presence of this variant in non-O157 STEC isolates from calf origin in our country.

Autotransporter protein Ag43 has been linked to pathogenic O157 and non-O157 E. coli strains and is associated with autoaggregation, cell-cell interaction with the host and biofilm formation (Matheus-Guimarães et al., 2014). It has been observed that the main role of Ag43 depends on the presence of other virulence factors like adhesins, and the genetic background of the strain (Carter et al., 2017). In this work, both Ag43 subfamilies were detected. Subfamily II (the Calcium-binding Antigen43

Figure 1 Phylogenetic relationships of STEC isolates. The phylogenetic tree was generated using the UPGMA method, using the Dice coefficient with a 1.0% of band position tolerance (BioNumerics v.6.6, Applied Maths, Sint-Martens-Latem, Belgium). Salmonella Braenderup and Staphylococcus aureus were used as reference strains. 

Homologue, Cah) was detected in 14 isolates, while two STEC isolates were Ag43 subfamily I, positive. It has been observed that the gene that codes for subfamily II (cah) has a high mutation rate, which is associated with the adaptability of STEC to different environments (Carter et al., 2017). On the other hand, Ag43 subfamily I, is not present in LEE+ strains since it is encoded in LAA pathogenicity island (Locus of Adhesion and Autoaggregation) (Montero et al., 2017). Primers to detect both subfamilies used in this work were designed based on a difference of 270bp between them, and subfamily I could not be found in STEC LEE+ isolates. Therefore, it is probable that our STEC LEE+ isolates present heterogenicity among this gene, which may be due to distinct functions that could favour an in-host or environmental status.

The major serogroup associated with STEC infections in humans still is E. coli O157:H7, the first serogroup recognised causing enteric bloody diarrhoea (EFSA BIOHAZ Panel, 2020). However, other STEC serogroups are now recognised as important food and water-borne pathogens. Among them, the “big six” group of strains is frequently detected in HUS cases (Shridhar et al., 2017). In this work, O103 and O111 were the only serogroups detected. Five STEC isolates were ascribed to the O103 serogroup (three isolates from a bovine with NCD signs and two isolates from a calf without signs) whereas six isolates were ascribed to O111. The remaining five isolates could not be assigned to any of the evaluated serogroups (31.3% of the isolates). Considering that there are over 1150 published STEC serotypes, these isolates probably could be assigned to other than the “big six”. O103 and O111 serogroups have been previously determined in cattle (Thomas et al., 2012, Bibbal et al., 2015, Jajarmi et al., 2017, Rivelli Zea et al., 2020), their faeces (Blanco et al., 2004, Cernicchiaro et al., 2013) and carcasses (Cap et al., 2019), in countries from South America and other regions. On the other hand, the isolation of STEC O111 in bloody diarrhoea cases has been reported in our country (Varela et al., 2008), which demonstrates a previous circulation of this serogroup. Also, a positive correlation between the presence of the virulence-marker gene eae in non-O157 STEC and the occurrence of HUS have been established (Yang et al., 2020b), an affirmation that agrees with the assumption that non-O157 STEC LEE+ isolates from this study could be harmful to human.

It is well known that E. coli isolates from different sources of isolation usually belong to different phylogroups (Clermont et al., 2013). Therefore, it would be expected that the STEC isolates of bovine origin of this work all belong to the same phylogroup. However, five different phylogroups were determined. According to the molecular assignment, 50% (8/16) of the isolates were classified as B1. This observation is consistent with reports which indicate that B1 is mainly present in the microbiota of domestic animals, often associated with intestinal commensal and pathogenic E. coli (Tenaillon et al., 2010). The second most frequently detected phylogroup was E (4/16). It has recently been reported that this phylogroup predominantly includes O157:H7 strains (Tenaillon et al., 2010). The A phylogroup has been also associated with commensal/ intestinal pathogens, and in this work was assigned to 2/16 STEC isolates. Finally, C and F phylogroups were each represented with 1/16 of the isolates. Both phylogroups have been proposed to be sister groups of B1 and B2, respectively (Clermont et al., 2013).

When the clonal relatedness of the isolates was analysed, a high level of diversity amongst isolates was observed. PFGE profiles showed 13 distinct restriction patterns out of 16 STEC isolates. We only found two indistinguishable band patterns within animals (the pair of isolates: AD1.5 and AD1.6 / AC3.1 and AC3.10 came each from one animal), and within a bovine herd (isolates AD1.7 and AD 7.2 came from different animals but from the same herd). Further, more than one band pattern was detected within bovine herds. When we looked out serogroups, we detected that STEC isolates ascribed to the O103 were closely related; and those ascribed to the O111 serogroup were closely related too. Similar results from PFGE analyses were previously determined in STEC isolates from cattle origin (Bibbal et al., 2015, Bumunang et al., 2019), which reaffirms the role of bovine as non-O157 STEC strains reservoirs.

It has been observed that antibiotic resistance in non- O157 STEC isolates from animal origin is higher than in O157 STEC strains (Mir & Kudva, 2019). Likewise, multidrug resistance is frequently associated with non-O157 STEC strains with eae+/stx1+ virulence profiles (Mora et al., 2015). In the present study, 11 antibiotics were evaluated using the Kirby-Bauer method. AMP resistance was the most frequent amongst isolates (62.5%), and it was determined in the following arrangements: AMP (n=7), AMP/CIP (n=2), and AMP/CN/FOT (n=1) being the last one the only isolate considered MDR. Finally, one isolate was only FOTR and 1 isolate was only CIPR. The high percentage of resistance to AMP agrees with the fact that β-lactams are the most used antibiotics in animals and with previous reports by our group, although the antibiotic resistance profiles then were considerably higher in numbers and included resistance to cephalosporins and trimethoprim-sulfamethoxazole among others (Umpiérrez et al., 2017, 2021). Even though isolates were in general susceptible to the tested antibiotics, 5/12 of the resistant isolates were also classified as O111 and 3/12 were classified as O103. In previous works, O111 serogroup has been associated with multidrug-resistant non-O157, eae+ STEC isolates (Mora et al., 2015). Particularly it has been associated with resistance levels higher than 25% to ampicillin, amoxicillin-clavulanic acid, cephalothin, trimethoprim-sulfamethoxazole, tetracycline, chloramphenicol and streptomycin. Meanwhile, O103 STEC isolates have been associated with low resistance levels, except for trimethoprim-sulfamethoxazole, tetracycline and streptomycin (Schroeder et al., 2002, Amézquita-López et al., 2016). Even though non-O157 STEC isolates of this study were mainly susceptible to antibiotics, it is important to state that they were screened from a large collection of E. coli isolates, in which the percentage of antibiotic resistance is significant (Umpiérrez et al., 2017). In that collection, MDR isolates represented a substantial source for antibiotic resistance genes. In this study, we also detected the presence of the fosA7 gene (STEC isolate 16.16), which confers resistance to fosfomycin. The presence of this gene has been reported in environmental E. coli isolates and Salmonella spp. from animal and human origin (Rehman et al., 2017, Balbin et al., 2020). Resistance to fosfomycin is a major concern in human health. In our country, on the one hand, it is within the scarce therapeutic resources available for infections of multi-resistant microorganisms (Seija et al., 2015) and, on the other, it is one of the first therapeutic options for urinary tract infections (García-Fulgueiras et al., 2021). Recently, we have reported the presence of fosA3 in animals belonging to the food-production chains (Coppola et al., 2020). With this scenario, the detection of fosA7 in production chain animals reinforces the need to monitor the presence of this mechanism with a One Health concept. To the best of our knowledge, this is the first study to report on the fosA7 gene in STEC from bovines in Uruguay. Considering the critically important antimicrobials for human medicine list published periodically by WHO (World Health Organization & WHO Advisory Group on Integrated Surveillance of Antimicrobial Resistance, 2017), dissemination of resistance to such antibiotics through non-O157 STEC isolates of calf origin did not seem to be of human or animal health concern.

It is concluded that studies encompassing molecular characterisation of non-O157 STEC of calf origin are on the rise, due to an increase in detecting these serogroups associated with severe disease in humans. Although in this study the number of evaluated isolates from cattle was low, the virulence profiles, including the confirmation of Shiga toxin subtypes stx1a and stx2a, and serogroups O103 and O111 (the last one, has been reported in human infections in our country), denote the harmful potential of them for humans. We also observed some genetic heterogeneity among the Ag43 gene, which could be associated with adaptation to different niches. Finally, circulating non-O157 STEC LEE+ from cattle faeces were not from one cluster only and showed high genetic heterogeneity, being in general susceptible to antibiotics. Further investigations with a higher number of STEC are needed to confirm these observations.

The spread of STEC isolates to the environment through bovine faeces is always a source of concern to human health, but also represents a way to contaminate the dairy environment, which increases the probability of bovine infections within a herd. Results from this study call for attention regarding the virulence profile and transferable resistance genes of non-O157 STEC LEE+ isolates, that could cause severe disease to humans and denote the essentiality of determining how they persist and transmit in the dairy environment.


The authors declare that they have no competing interests.


No ethical approval was required in this work, as this is an original article with only bacterial isolates and data. No animal or human samples were employed.


We are incredibly grateful to all the veterinarians and farmers who collaborated in the sample collection. The authors especially thank the research group from “Plataforma de Salud Animal” at INIA La Estanzuela for their outstanding work in the field with sample collection and primary isolation of E. coli. Also, we are thankful to Cecilia Monesiglio for her collaboration in E. coli isolation.


Amézquita-López, B.A., Quiñones, B., Soto-Beltrán, M., Lee, B.G.,Yambao, J.C., Lugo-Melchor, O.Y., & Chaidez, C. (2016). Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico. Antimicrobial Resistance & Infection Control, 5(1). ]

Bai, X., Fu, S., Zhang, J., Fan, R., Xu, Y., Sun, H., He, X., Xu, J., & Xiong, Y. (2018). Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype. Scientific Reports, 8, 6756. 10.1038/s41598-018-25233-x [ Links ]

Balbin, M.M., Hull, D., Guest, C., Nichols, L., Dunn, R., Hull, D.,& Siddhartha, T. (2020). Antimicrobial resistance and virulence factors profile of Salmonella spp. and Escherichia coli isolated from different environments exposed to anthropogenic activity. Journal of Global Antimicrobial Resistance, 22, 578-583. https:// ]

Bettelheim, K., & Goldwater P. 2014. Serotypes of Non-O157 Shigatoxigenic Escherichia coli (STEC). Advances in Microbiology, 4, 377-389. ]

Beutin, L., Krüger, U., Krause, G., Miko, A., Martin, A., & Strauch,E. (2008). Evaluation of major types of Shiga toxin 2E-producing Escherichia coli bacteria present in food, pigs, and the environment as potential pathogens for humans. Applied and Environmental Microbiology, 74(15), 4806-4816. ]

Bibbal, D., Loukiadis, E., Kérourédan, M., Ferré, F., Dilasser, F., Peytavin de Garam, C., Cartier, P., Oswald, E., Gay, E., Auvray, F., & Brugère, H. (2015). Prevalence of carriage of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among slaughtered adult cattle in France. Applied and Environmental Microbiology, 81(4), 1397-1405. https://doi. org/10.1128/AEM.03315-14Links ]

Blanco, M., Padola, N.L., Krüger, A., Sanz, M.E., Blanco, J.E., González, E.A., Ghizlane, D., Azucena, M., Bernárdez, M.I., Etcheverría, A.I., Arroyo, G.H., Lucchesi, P.M.A., Parma, A.E., & Blanco, J. (2004). Virulence genes and intimin types of Shiga-toxin-producing Escherichia coli isolated from cattle and beef products in Argentina. International Microbiology, 7(4), 269-276. [ Links ]

Bumunang, E.W., McAllister, T.A., Zaheer, R., Polo, R.O., Stanford, K., King, R., Niu, Y.D., & Ateba, C.N. (2019). Characterization of non-O157 Escherichia coli from cattle faecal samples in the North- West Province of South Africa. Microorganisms, 7(8), 272. ]

Cap, M., Carbonari, C.C., D’Astek, B.A., Zolezzi, G., Deza, N., Palladino, M.P., Masana, M., Chinen, I., & Rivas, M. (2019). Frequency, characterization and genotypic analysis of Shiga toxin-producing Escherichia coli in beef slaughterhouses of Argentina. Revista Argentina de Microbiología, 51(1), 32-38. ram.2018.03.005Links ]

Carter, M.Q., Brandl, M.T., Kudva, I.T., Katani, R., Moreau, M.R., & Kapur, V. (2017). Conditional function of autoaggregative protein cah and common cah mutations in Shiga toxin-producing Escherichia coli. Applied and Environmental Microbiology, 84(1), e01739-17. ]

Cernicchiaro, N., Cull, C.A., Paddock, Z.D., Shi, X., Bai, J., Nagaraja, T.G., & Renter, D.G. (2013). Prevalence of Shiga toxin-producing Escherichia coli and associated virulence genes in feces of commercial feedlot cattle. Foodborne Pathogens and Disease, 10(10), 835-841. ]

Clermont, O., Christenson, J.K., Denamur, E., & Gordon, D.M. (2013). The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environmental Microbiology Reports, 5(1), 58-65. ]

Clinical and Laboratory Standards Institute. (2017). Performance Standards for Antimicrobial Susceptibility Testing. CLSI supplement M100. [ Links ]

Cóppola, N., Freire, B., Umpiérrez, A., Cordeiro, N.F., Ávila, P., Trenchi, G., Castro, G., Casaux, M.L., Fraga, M., Zunino, P., Bado, I., & Vignoli, R. (2020). Transferable resistance to highest priority critically important antibiotics for human health in Escherichia coli strains obtained from livestock feces in Uruguay. Frontiers in Veterinary Science, 7, 588919. ]

Daly, R.F., & Hill, N.T. (2016). Characterizing the role of animal exposures in cryptosporidiosis and Shiga toxin-producing Escherichia coli infections: South Dakota, 2012. Zoonoses and Public Health, 63(6), 467-476. [ Links ]

EFSA BIOHAZ Panel. (2020). Pathogenicity assessment of Shiga toxin- producing Escherichia coli (STEC) and the public health risk posed by contamination of food with STEC. EFSA Journal 18, e05967. ]

Fan, R., Shao, K., Yang, X., Bai, X., Fu, S., Sun, H., Xu, Y., Wang, H., Li, Q., Hu, B., Zhang, J., & Xiong, Y. (2019). High prevalence of non-O157 Shiga toxin-producing Escherichia coli in beef cattle detected by combining four selective agars. BMC Microbiology, 19(1), 213. ]

Gadea, M., Deza, N., Mota, M.I., Carbonari, C., Robatto, M., D`Astek, B., Balseiro, V., Bazet, C., Rügnitz, E., Livrelli, V., Schelotto, F., Rivas, M., & Varela, G. (2012). Two cases of urinary tract infection caused by Shiga toxin-producing Escherichia coli O157:H7 strains. Revista Argentina de Microbiología, 44(2), 94-96. [ Links ]

García-Fulgueiras, V., Caiata, L., Bado, I., Giachetto, G., Robino, L. (2021). Antibiotic susceptibility and fosfomycin resistance characterization in a cohort of children older than 6 years of age with urinary tract infection. Revista Argentina de Microbiología. ]

Gyles, J.M., & Fairbrother, F.C.L. (2010). Escherichia coli. In C.L. Gyles, J.F. Prescott, J.G. Songer, & C.O. Thoen, (Eds.), Pathogenesis of Bacterial Infections in Animals (pp. 276-308). Wiley-Blackwell. [ Links ]

Jajarmi, M., Imanifooladi, A.A., Badouei, M.A., & Ahmadi, A. (2017). Virulence genes, Shiga toxin subtypes, major O-serogroups, and phylogenetic background of Shiga toxin-producing Escherichia coli strains isolated from cattle in Iran. Microbial Pathogenesis, 109, 274-279. ]

Kintz, E., Brainard, J., Hooper, L., & Hunter, P. (2017). Transmission pathways for sporadic Shiga-toxin producing E. coli infections: A systematic review and meta-analysis. International Journal of Hygiene and Environmental Health, 220(1), 57-67. https://doi. org/10.1016/j.ijheh.2016.10.011Links ]

Kjaergaard, K., Schembri, M.A., Hasman, H., & Klemm, P. (2000). Antigen 43 from Escherichia coli induces inter- and intraspecies cell aggregation and changes in colony morphology of Pseudomonas fluorescens. Journal of Bacteriology, 182(17), 4789-4796. https:// ]

Majowicz, S.E., Scallan, E., Jones-Bitton, A., Sargeant, J.M., Stapleton, J., Angulo, F.J., Yeung, D.H., & Krik, M.D. (2014). Global incidence of human Shiga toxin-producing Escherichia coli infections and deaths: a systematic review and knowledge synthesis. Foodborne Pathogens and Disease, 1, 447-455. [ Links ]

Marshall, B.M., & Levy, S.B. (2011). Food Animals and Antimicrobials: Impacts on Human Health. Clinical Microbiology Reviews, 24(4), 718-733. ]

Matheus-Guimarães, C., Gonçalves, E.M., & Guth, B.E.C. (2014). Interactions of O157 and non-O157 Shiga toxin-producing Escherichia coli (STEC) recovered from bovine hide and carcass with human cells and abiotic surfaces. Foodborne Pathogens and Disease, 11(3), 248-255. ]

Mir, R.A., & Kudva, I.T. (2019). Antibiotic-resistant Shiga toxin-producing Escherichia coli: An overview of prevalence and intervention strategies. Zoonoses and Public Health, 66(1), 1-13. https://doi. org/10.1111/zph.12533Links ]

Montero, D.A., Velasco, J., Del Canto, F., Puente, J.L., Padola, N.L., Rasko, D.A., Farfán, M., Salazar, J.C., & Vidal, R. (2017). Locus of Adhesion and Autoaggregation (LAA), a pathogenicity island present in emerging Shiga Toxin-producing Escherichia coli strains. Scientific Reports, 7, 7011. ]

Mora, A., Blanco, J.E., Blanco, M., Alonso, M.P., Dhabi, G., Echeita, A., González, E.A., Bernárdez, M.I., & Blanco, J. (2005). Antimicrobial resistance of Shiga toxin (verotoxin)-producing Escherichia coli O157:H7 and non-O157 strains isolated from humans, cattle, sheep and food in Spain. Research in Microbiology, 156(7), 793-806. ]

Paddock, Z., Shi, X., Bai, J., & Nagaraja, T.G. (2012). Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces. Veterinary Microbiology, 156(3-4), 381-388. vetmic.2011.11.017Links ]

Patel, J.B. (2014). Performance Standards for Antimicrobial Susceptibility Testing (24th ed). Clinical and Laboratory Standards Institute. [ Links ]

Pérez, L., Apezteguía, L., Piñeyrúa, C., Dabezies, A., Bianco, M.N., Schelotto, F., & Varela, G. (2014). Hemolytic uremic syndrome with mild renal involvement due to Shiga toxin-producing Escherichia coli (STEC) O145 strain. Revista Argentina de Microbiología, 46(2), 103-106. ]

Rehman, M.A., Yin, X., Persaud-Lachhman, M.G., & Diarra, M.S. (2017). First detection of a fosfomycin resistance gene, fosA7, in Salmonella enterica serovar Heidelberg isolated from broiler chickens. Antimicrobial Agents and Chemotherapy, 61(8), e00410-17. https:// ]

Rivas, M., Chinen, I., Miliwebsky, E., & Masana, M. (2014). Risk Factors for Shiga Toxin-Producing Escherichia coli-Associated Human Diseases. Microbiology Spectrum, 2(5). microbiolspec.EHEC-0002-2013Links ]

Rivelli Zea, S.M., Padola, N.L., Etcheverría, A.I, Florentín, M., Acuña, P., Rodríguez, F., Colello, R., & Guillén Fretes, R.M. (2020). Caracterización molecular de aislamientos de Escherichia coli productores de toxina Shiga obtenidos en 2 establecimientos ganaderos del Paraguay. Revista Argentina de Microbiología, 52(2), 131-135. ]

Sapountzis, P., Segura, A., Desvaux, M., & Forano, E. (2020). An overview of the elusive passenger in the gastrointestinal tract of cattle: The Shiga toxin producing Escherichia coli. Microorganisms, 8(6), 877. ]

Scheutz, F., Teel, L.D., Beutin, L., Piérard, D., Buvens, G., Karch, H., Mellmann, A., Caprioli, A., Tozzoli, R., Morabito, S., Strockbine, N.A., Melton-Celsa, A.R., Sanchez, M., Persson, S., & O’Brien,A.D. (2012). Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. Journal of Clinical Microbiology, 50(9), 2951-2963. https://doi. org/10.1128/JCM.00860-12Links ]

Scheutz, F. (2014). Taxonomy meets public health: The case of Shiga toxin-producing Escherichia coli. Microbiology Spectrum, 2. https:// ]

Schroeder, C.M., Meng, J., Zhao, S., DebRoy, C., Torcolini, J., Zhao, C., McDermott, P.F., Wagner, D.D., Walker, R.D., & White, D.G. (2002). Antimicrobial resistance of Escherichia coli O26, O103, O111, O128, and O145 from animals and humans. Emerging Infectious Diseases, 8(12), 1409-1414. ]

Seija, V., Medina Presentado, J.C., Bado, I., Papa Ezdra, R., Batista, N., Gutiérrez, C., Guirado, M., Vidal, M., Nin, M., & Vignoli, R. (2015). Sepsis caused by New Delhi metallo-β-lactamase (blaNDM-1) and qnrD-producing Morganella morganii, treated successfully with fosfomycin and meropenem: case report and literature review. International Journal of Infectious Diseases, 30, 20-26. https://doi. org/10.1016/j.ijid.2014.09.010Links ]

Shridhar, P.B., Siepker, C., Noll, L.W., Shi, X., Nagaraja, T.G., & Bai,J. (2017). Shiga toxin subtypes of non-O157 Escherichia coli serogroups isolated from cattle feces. Frontiers in Cellular and Infection Microbiology, 7. ]

Tenaillon, O., Skurnik, D., Picard, B., & Denamur, E. (2010). The population genetics of commensal Escherichia coli. Nature Reviews Microbiology, 8(3), 207-217. ]

Thomas, K.M., McCann, M.S., Collery, M.M., Logan, A., Whyte, P., McDowell, D.A., & Duffy, G. (2012). Tracking verocytotoxigenic Escherichia coli O157, O26, O111, O103 and O145 in Irish cattle. International Journal of Food Microbiology 153(3), 288-296. https:// ]

Torres, A.G., Amaral, M.M., Bentancor, L., Galli, L., Goldstein, J., Krüger, A., & Rojas-López, M. (2018). Recent advances in Shiga toxin-producing research in Latin America. Microorganisms, 6(4),100. ]

Umpiérrez, A., Bado, I., Oliver, M., Acquistapace, S., Etcheverría, A.L., Padola, N.L., Vignoli, R., & Zunino, P. (2017). Zoonotic Potential and Antibiotic Resistance of Escherichia coli in Neonatal Calves in Uruguay. Microbes Environ, 32(3), 275-282. jsme2.ME17046Links ]

Umpiérrez, A., Ernst, D., Fernández, M., Oliver, M., Casaux, M.L., Caffarena, R.D., Schild, C., Giannitti, F., Fraga, M., & Zunino, P. (2021). Virulence genes of Escherichia coli in diarrheic and healthy calves. Revista Argentina de Microbiología, 53(1), 34-38. https:// ]

Varela, G., Chinen, I., Gadea, M.P., Miliwebsky, E., Mota, M.I., González, S., González, G., Gugliada, M.J., Carbonari, C.C., Algorta, G., Bernadá, M., Sabelli, R., Pardo, L., Rivas, M., & Schelotto, F. (2008). Detección y caracterización de Escherichia coli productor de toxina Shiga a partir de casos clínicos y de alimentos en Uruguay. Revista Argentina de Microbiología, 40(2), 93-100. [ Links ]

World Health Organization & WHO Advisory Group on Integrated Surveillance of Antimicrobial Resistance. (2017). Critically important antimicrobials for human medicine: ranking of antimicrobial agents for risk management of antimicrobial resistance due to non-human use, 5th rev. World Health Organization. handle/10665/255027. [ Links ]

Yang, X., Bai, X., Zhang, J., Sun, H., Fu, S., Fan, R., He, X., Scheutz, F. Matussek, A. (2020a). Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China. International Journal of Medical Microbiology, 310(1), 151377. ijmm.2019.151377Links ]

Yang, X., Sun, H., Fan, R., Fu, S., Zhang, J., Matussek, A., Xiong,Y., & Bai,X. (2020b). Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China. Scientific Reports, 10, 3275. ]


This work was supported by Red de Macrouniversidades (IX Convocatoria de Movilidad, 2017) and the FONDECYT grant (1161161).

Recibido: 12 de Octubre de 2021; Aprobado: 30 de Diciembre de 2021

*Corresponding author: A. Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay;

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