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Austral journal of veterinary sciences

versión impresa ISSN 0719-8000versión On-line ISSN 0719-8132

Austral j. vet. sci. vol.50 no.3 Valdivia  2018

http://dx.doi.org/10.4067/S0719-81322018000300135 

SHORT COMMUNICATION

Report of first isolation of the zoonotic Arcobacter species from swine fecal samples in Ecuador

Rosa Janneth SimaluizaA 

Jimmy FernándezA 

Heriberto FernándezB 

A Departamento de Ciencias de la Salud, sección de Genética Humana, Microbiología y Bioquímica Clínica, Universidad Técnica Particular de Loja, Loja, Ecuador.

B Facultad de Medicina, Instituto de Microbiología Clínica, Universidad Austral de Chile, Valdivia, Chile.

Abstract:

Arcobacter butzleri, A. cryaerophilus , A. skirrowii and A. thereius are recognised as emerging zoonotic agents, recovered from animals and human beings. The information available about Arcobacter species in Latin America is scarce. Among domestic animals, swine are considered important reservoir and source of contamination of different Arcobacter species. This communication reports the first simultaneous isolation of the four zoonotic Arcobacter species in Ecuador. Preliminary identification was done by phenotypic characteristics and definitive species identification was made by multiplex PCR method. Further investigation about the prevalence, distribution, ecology and interactions with human beings of these species is required.

Keywords: Arcobacter; zoonotic species; reservoir; Ecuador

Introduction

The genus Arcobacter, included into the family Campylobacteraceae, class Proteobacteria, subclass Gracillicutes, comprises aero-tolerant, curved or spiral rod shaped and polar flagellated bacteria, firstly described as Vibrio/Spirillum organisms and later as aero-tolerant Campylobacter-like organisms (Vandamme 2000, Fernández et al 1995, Collado and Figueras 2011).

Nowadays, genus Arcobacter comprises 27 validated species showing diverse habitats, being A. canalis the last described (Perez-Cataluña et al 2018). Some species live in association with a wide diversity of animals while others are found in environmental sources (Collado and Figueras 2011, Ferreira et al 2017). Only four of these species, A. butzleri, A. cryaerophilus, A. skirrowii and A. thereius are recognised as emerging zoonotic agents, being recovered from animals and human beings (Vandenberg et al 2004, Lehner et al 2005, Silha et al 2015, van den Abeele et al 2015, Ferreira et al 2017).

It is important to highlight that among domestic ani mals, swine are considered important hosts, reservoir and source of contamination of different Arcobacter species (Ho et al 2006), and that in Latin America the information available about Arcobacter species is scarce. These bacteria have been isolated only in Chile (Fernández et al 1995), Argentina (Giacoboni et al 1997), Brazil (de Oliviera et al 1999), Mexico (Villarruel-López et al 2003), Peru (Zerpa et al 2015) and Costa Rica (Bogantes et al 2015). Thus, we conducted a pilot study in order to establish the presence of Arcobacter species in faecal samples obtained of healthy pigs at slaughterhouse.

Material and methods

Twenty faecal samples were randomly taken by rectal swabs from healthy pigs, before the beginning of the slaughter at the slaughterhouse of Loja city, Southern Ecuador (3°59’ Lat S; 79°12’ Long W).

To isolate Arcobacter sp., each sample was seeded into CAT (cefaperazone, amphotericin B and teicoplanin) enrichment broth and incubated at 30 °C for 72 h under aerobic conditions (Collado et al 2013), followed by passive filtration through 0.45 pm membrane filter placed on blood agar plates. After 30 min filtration, the filters were removed and the plates were incubated under the same conditions described above (Collado et al 2013, Fernández et al 2015). Preliminary identification of isolates was carried out by phenotypic characteristics [curved Gram negative rod, motility +, aerobic growth +, oxidase + and catalase + (Fernández et al 2015)]. Definitive species identification was done by the multiplex PCR (mPCR) method propo sed by Douidah et al (2010) that characterizes the 5 most common Arcobacter species.

Results and discussion

Among the 20 faecal samples studied, three of them (15%) were positive for Arcobacter sp. One yielded A. cryaerophilus, the second A. skirrowii and the third, A. butzleri and A. thereius simultaneously. These four species have been isolated from pigs in different parts of the world, including in some Latin American countries (Collado et al 2013, Fernández et al 2015, Barboza et al 2017, Ferreira et al 2017). However, so far as we know, in Latin America these four zoonotic have been reported simultaneously only in Costa Rica, especially in samples from poultry and food of animal origin (Barboza et al 2017).

The isolation rate (15%) found in this pilot study falls within the ranges reported by De Smet et al (2011) in four Belgian pig farms where the isolation rates were 11.3, 15.6, 15.0 and 50.0%, and the isolated species were A. butzleri, A. cryaerophilus, A. skirrowii, A. thereius and A. trophiarum. However, it is higher than the 4% reported by Gobby et al (2018) in Brazil. The diversity of species found by these authors was restricted only to A. butzleri and A. cryaerophilus.

Arcobacter butzleri, A. cryaerophilus, A. skirrowii and A. thereius are currently recognised as emerging zoonotic agents isolated from different animals and human beings (Vandenberg et al 2004, Lehner et al 2005, Silha et al 2015, van den Abeele et al 2015, Ferreira et al 2017). Since their main routes of transmission to humans are the consumption and handling of raw or undercooked foods of animal origin like meats, milk, seafood and water, they are also considered as potential foodborne patho gens (Vandenberg et al 2004, Lehner et al 2005, Collado et al 2013, Fernández et al 2015, Silha et al 2015, van den Abeele et al 2015, Ferreira et al 2017). From these species, A. butzleri is the most frequently isolated from animal, environmental and human samples, followed by A. cryaerophilus, being both associated with abortion and enteritis in animals as well as with diarrhea and bacteremia in children and adults (Collado et al 2013, Silha et al 2015, van den Abeele et al 2015, Ferreira et al 2017, Barboza et al 2017). Arcobacter skirrowii has been isolated from sheep and cattle with diarrhea, aborted pig foetus, and chronic and acute diarrhea in humans (Lehner et al 2005, Collado et al 2013, van den Abeele et al 2015, Ferreira et al 2017) whereas A. thereius has been isolated from pig’s faecal samples, duck’s cloacal samples and from livers and kidneys of pigs with spontaneous abortions. More recently, it was isolated from stool of hospitalised patients with diarrhoea (van den Abeele et al 2015, Ferreira et al 2017, Rovetto et al 2017).

In addition to these four zoonotic species of Arcobacter, other species of the genus have been isolated from pigs, such as A. thereius, A. throphiarum, A. suis and A. lanthieri (Whiteduck-Léveillée et al 2015). More recently Figueras et al (2017) established that A. thereius included a group strains (represented by strain LMG 24487) that clustered separately from the type strain (LMG 24486T) representing the new species Arcobacter porcinus. However, none of these species, except A. thereius, has been isolated from human beings until now. Bearing in mind that the zoonotic species of Arcobacter are considered as emerging species in human pathology, it is necessary to pay attention to the eventual presence of these other species in human clinical samples. Also, in the case of the isolation to A. thereius, it becomes necessary to differentiate it with A porcinus. The PCR method used in this work (Douidah et al 2010) cannot differentiate A. porcinus from A. thereius, because it was developed before the proposal of Figueras et al (2017).

This communication reports the first simultaneous isolation of the four zoonotic Arcobacter species in

Ecuador allowing the identification of swine as one of their reservoir in this country.

Considering the zoonotic, emergent and foodborne character of these bacteria, we believe that it is important to establish their presence in other animal sources as well as in foods of animal origin, environmental waters and humans. This information will contribute to a better unders tanding of the epidemiology of these emerging foodborne enteropathogens as well as to evaluate the aplication of control actions from a food safety point of view.

Acknowledgments:

This work was financially supported by grant UTPL PROY_ CCSAL_1283.

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Accepted: August 09, 2018

Corresponding author: H Fernández; hfernand@uach.cl

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