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Revista chilena de cardiología

On-line version ISSN 0718-8560

Abstract

RAMOS, Elizabeth et al. Over expression of the homologous angiotensin converting enzyme (ACE2) gene reverts experimental hypertensión and cardiac remodeling. Rev Chil Cardiol [online]. 2010, vol.29, n.3, pp.334-341. ISSN 0718-8560.  http://dx.doi.org/10.4067/S0718-85602010000300008.

Objective Background. Over expression of the gene for homologous angiotensin I converting enzyme (ACE) is associated with prevention of angiotensin II dependent cardiac hypertrophy and fibrosis. Whether this over expression is able to reduce hypertension and revert cardiac remodeling is unknown. Aim: To determine whether adenoviral ACE2 gene over expression in the myocardium decreases experimental hypertension and cardiac remodeling in rats with genetically determined levéis of ACE and ANGII. Methods: Homozygous normotensive Lewis (LL) and Brown Norway (BN) rats with decreased or increased levéis of ACE and Ang II, respectively, were made hy-pertensive using the Goldblatt procedure. Sham opera-ted rats were used as control s. 5 weeks after surgery, when systolic blood pressure reached > 140 mmHg, rats were randomized to receive intramyocardial injec-tion of either AdACE2 or green fluorescent Ad protein (GFP) as infection controlling agents. One week after adenoviral infection, rats were sacrificed. Body weight (BW, Gr), relative cardiac mass (RCM, mG), systolic blood pressure (SBP, mmHg), cardiomyocite área (MA um2) and perimeter (MPER, uM), and myocardial co-llagen content, both subendocardial (SC, %) and total (TC %) were measured. Results: Hypertension was associated to significant in-creases in total and RCM, MA, MPER as well as SC and TC in Goldblatt vs normal rats. This was indepen-dent of BW and not affected by ACE polymorphism. ACE II over expression significantly decreased SBP (27 vs 15%), MA (25 vs 25%) and MPER (18 vs 17%) hy-pertensive vs normal rats, respectively. Conclusión: Over expression of ACE2 decreased hypertension, hypertrophy and fibrosis in rats with experimental hypertension and different levéis of ACE and Ang II. (Fondecyt 1070662).

Keywords : ACE2; adenoviral infection; hypertension; cardiac hypertrophy.

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