SciELO - Scientific Electronic Library Online

vol.79 issue3Soil carbon dioxide efflux in conilon coffee (Coffea canephora Pierre ex A. Froehner) plantations in different phenological phases in tropical climate in BrazilStudy of physiological and quality parameters during development and ripening of pepino (Solanum muricatum Aiton) fruit author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Chilean journal of agricultural research

On-line version ISSN 0718-5839


GONZALEZ-LAZARO, Mariel et al. PCR diagnosis and in vitro sanitation of the papaya MSXJ hybrid with ringspot virus disease. Chil. j. agric. res. [online]. 2019, vol.79, n.3, pp.376-384. ISSN 0718-5839.

Papaya (Carica papaya L.) is a tropical fruit with a high nutritional value. Current problems affecting its cultivation are the limited number of exploited varieties and its susceptibility to pests and diseases. The papaya MSXJ hybrid is resistant to pests and diseases, and its organoleptic characteristics are prominent compared with most papaya genotypes on the market. Samples of Papaya ringspot virus (PRSV) disease were evaluated by in vitro culture of the apical meristems and thermotherapy. Sanitation of apical meristems was accomplished by regenerating them with or without light and thermotherapy was performed at 40 and 45 °C for 4 and 7 h, respectively. Both situations were observed for 45 d to evaluate survival, regeneration, and sanitation by reverse transcription polymerase chain reaction (RT-PCR). In vitro culture of the meristems in total darkness obtained the highest survival rate (87%) and regeneration (22%) compared with the treatments under photoperiod (21% and 18%, respectively). Under both study conditions, sanitation in PRSV was 100% of the meristems. Plants subjected to thermotherapy showed a higher survival rate (99.92%) at 40 °C for 4 h and sanitation of 80.2%

Keywords : Apical meristems; Carica papaya; Papaya ringspot virus (PRSV); RT-PCR; thermotherapy.

        · text in English     · English ( pdf )