SciELO - Scientific Electronic Library Online

 
vol.45 número4Modelling fishery-induced impacts on the food web of the continental shelf off central-south Chile using a size-based network approachReproductive biology of Macrobrachium amazonicum (Heller, 1862) (Decapoda: Palaemonidae) in a reservoir situated in Minas Gerais State, southeastern Brazil índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Latin american journal of aquatic research

versión On-line ISSN 0718-560X

Resumen

OPAZO, Rafael; VALLADARES, Luis  y  ROMERO, Jaime. Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings. Lat. Am. J. Aquat. Res. [online]. 2017, vol.45, n.4, pp.766-775. ISSN 0718-560X.  http://dx.doi.org/10.3856/vol45-issue4-fulltext-12.

Variable individual growth rate is a phenomenon observed in fish cohorts that influences the aquaculture performance and fish cohort ecological viability. Our aim was to compare gene expression patterns of key growth genes in zebrafish larvae with different growth rate. The body length of sibling zebrafish larvae at 6 days post hatching (dph) was measured. The larvae were reared to 20 dph and measured again. Two body-length groups were clearly observed: 4 mm (small larvae) and 5-6 mm (large larvae). Total RNA was isolated from both groups. Growth hormone (gh), growth hormone receptor (ghr), insulin-like growth factor 1 (igf-1), insulin-like growth factor receptor (igf-1r a/b), insulin-like growth factor binding protein 1 (igfbp-1), thyroglobulin (tg), cholecystokinin (cck), and ghrelin were evaluated by quantitative polymerase chain reaction (qPCR). Glucokinase (gck) and igfbp-1 were included as a gene expression marker of larvae nutritional status. Two genes showed significant differences between the body length groups, igfbp-1 (P = 0.01) and igf-1r (P = 0.02). The igfbp-1 suggests than growth rate variability was associated with the larvae nutritional status and this condition affect the gene expression pattern of igf-1r. Therefore these genes are interesting genes markers for growth rate variabilities studies.

Palabras clave : Danio rerio; zebrafish; growth rate; IGF-1R; IGFBP-1; aquaculture.

        · texto en Inglés     · Inglés ( pdf )