SciELO - Scientific Electronic Library Online

 
vol.44 número4Biocenosis de la comunidad bentónica en la Laguna Guerrero Negro, Baja California Sur, México: caracterización espacio-temporalEvaluación de la primera madurez sexual del huachinango del Pacífico (Lutjanus peru) nacido en cautiverio índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Latin american journal of aquatic research

versão On-line ISSN 0718-560X

Resumo

STRAHSBURGER, Erwin; RETAMALES, Patricio; ESTRADA, Juan  e  SEEGER, Michael. Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture. Lat. Am. J. Aquat. Res. [online]. 2016, vol.44, n.4, pp.742-749. ISSN 0718-560X.  http://dx.doi.org/10.3856/vol44-issue4-fulltext-9.

The microdot method is a downscaling methodology of traditional tenfold serial dilution procedure used in microbiology. The microdot method uses 100 µL for serial dilution and count colonies in a spot of 10 µL. In this study we counted colonies directly in a chromogenic agar plate to determine, at the same time, the presence and cell concentration of target bacteria required for sanitary monitoring of Chilean export fishery products. Due to among importers countries the most concerning bacteria included in sanitary monitoring are Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, we used the chromogenic agar; CHROMagar ECC, CHROMagar Listeria and Baird Parker agar, respectively. The results shows no differences between quantitative results obtained with microdot and traditional method during the quantification of a culture of Escherichia coli (1.5 L). The sensitivity and specificity of the microdot method in association with each chromogenic agar was demonstrated in vitro with reference strains. In addition, the usefulness in sanitary monitoring of aquaculture procedures was evaluated in Artemia salina tanks. This method did not detected sanitary problems in surface water. Although other colonies grown in the chromogenic agar plate, their morphological and chromogenic properties not correspond to Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, being identified as Salmonella enterica subsp. enterica, Microbacterium sp., Bacillus sp. and Staphylococcus pasteuri by 16S rRNA gene sequence analysis. Hence, we propose the microdot chromogenic method as a low cost, specific and reliable procedure for sanitary monitoring of aquaculture procedures.

Palavras-chave : microdot method; sanitary monitoring; aquaculture; chromogenic agar.

        · resumo em Espanhol     · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons