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Latin american journal of aquatic research

versión On-line ISSN 0718-560X


SAYES, Camila; LEYTON, Yanett  y  RIQUELME, Carlos E. Bacterium Pseudoalteromonas sp. potential probiotic for larval fish culture. Lat. Am. J. Aquat. Res. [online]. 2016, vol.44, n.1, pp.76-84. ISSN 0718-560X.

ABSTRACT. Seriola lalandi is a pelagic marine species with high market demand national and internationally. However the larval survival in culture is low, which is attributed, among other factors, to the low quality of the larvae. The use of probiotic bacteria in appropriate amounts, in the larval culture of different organisms, has shown to improve the survival of the host. Based on this background our objective was to isolate and identify the bacteria from S. lalandi microbiota that show potential probiotic characteristics. For this purpose, 46 strains were isolated from S. lalandi juveniles and larvae, identified at the molecular level by analyzing the 16S rRNA gene. The following tests were performed as well: phylogenetic, antibacterial, hemolytic, proteolytic and lipolytic. The results showed that the total isolated bacteria, 42% belong to the genus Pseudoalteromonas, and nine presented inhibitory activity against pathogenic bacteria, of these, only one was negative for hemolysis, proteolysis and lipolysis. Based on the results, it is proposed to incorporate the bacteria Pseudoalteromonas sp. as potential probiotic adding it mixed with microalgae that are fed rotifers and artemia (vectors), which are traditionally used in the larval cultures of S. lalandi.

Palabras clave : Pseudoalteromonas sp.; Seriola lalandi; larvae; probiotic; inhibition; aquaculture.

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