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International journal of odontostomatology

versión On-line ISSN 0718-381X

Resumen

ARCE PANIAGUA, Marion; ULLOA CARMONA, Marcelo; POZO HERNANDEZ, Patricia  y  BRAVO BOWN, Joel. Porphyromonas gingivalis Detection in Adult Patient with Chronic Periodontitis. Int. J. Odontostomat. [online]. 2017, vol.11, n.1, pp.13-18. ISSN 0718-381X.  http://dx.doi.org/10.4067/S0718-381X2017000100002.

The detection of Porphyromonas gingivalis (P. gingivalis) was performed in adult patients with chronic periodontitis treated at the Clinic of Periodontology in the University of Antofagasta, through of microbiology techniques conventional and molecular biology. Were obtained samples of gingival crevicular fluid with paper cones (N 30) for 20 seconds of the more severe periodontal site in each quadrant, 37 patients were studied with chronic periodontitis, subsequent microbiological culture was performed on blood agar and the presence of P. gingivalis was detected through conventional microbiology techniques (fluorescence and stereomicroscope) and molecular biology technique (polymerase chain reaction). There was detected with stereomicroscope pigmented black in 75.7 % of the samples, of which 67.9 % were positive for P. gingivalis with the fluorescence technique. Through the polymerase chain reaction it was confirmed that 84.2 % of fluorescence positive colonies corresponded to P. gingivalis. In the population analyzed can conclude that there is a high prevalence of P. gingivalis in the subgingival microbiota of patients with chronic periodontitis which has been detected through techniques of conventional microbiology and by polymerase chain reaction. The smokers had a higher presence of P. gingivalis than nonsmokers.

Palabras clave : Periodontitis chronic; Porphyromonas gingivalis; Polymerase chain reaction.

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