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Idesia (Arica)

On-line version ISSN 0718-3429

Abstract

HUANCA-MAMANI, Wilson; SALVATIERRA MARTINEZ, Ricardo  and  SEPULVEDA-CHAVERA, Germán. A fast and efficient method for total DNA extraction from soil filamentous fungi. Idesia [online]. 2014, vol.32, n.2, pp.75-78. ISSN 0718-3429.  http://dx.doi.org/10.4067/S0718-34292014000200010.

Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

Keywords : DNA extraction; fungi; soil; PCR.

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