SciELO - Scientific Electronic Library Online

 
vol.39 issue3Sensitivity of wild-type and mutant Trichoderma harzianum strains tofungicidesEfficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Ciencia e investigación agraria

On-line version ISSN 0718-1620

Abstract

VIDAL, Alexis K; HAN, Dong-Sheng; NAKANO, Masaru  and  NIIMI, Yoshiji. Decreased time from seed to flowering corm size in Zephyra elegansvia in vitro cultivation. Cienc. Inv. Agr. [online]. 2012, vol.39, n.3, pp.577-584. ISSN 0718-1620.  http://dx.doi.org/10.4067/S0718-16202012000300017.

Experiments were performed to establish a method to reduce the time from seed to flowering in Zephyra elegans. Seedlings took at least four years to produce a flowering corm. Although germination was highest in a water-agar medium, plant necrosis occurred when plants were later transferred; therefore, MS (Murashige and Skoog) medium was selected as the medium for germination. An increase in the light intensity to 9500 lux and in the pH to 6.7, significantly increased the germination rate. Eight weeks after seed germination, seedlings were transferred to MS media with sucrose concentrations of 45, 60, 75, 90 or 105 g L-1 and a pH 5.7 or 6.7, with the aim of achieving the greatest corm weight gain. After 16 weeks, the best weight gain was obtained in the MS medium with 75 g L-1 of sucrose and a pH of 6.7. The resulting corms were transferred to pots and grown under greenhouse conditions. Corms weighing under 0.12 g did not sprout, whereas all corms over 0.3 g sprouted and all those over 0.4 g at the end of the in vitro culture stage bloomed in the second year of greenhouse cultivation. The time required for development from seedling to flowering corm was reduced to two years.

Keywords : Chilean geophyte; corm weight gain; flowering; seed propagation; Zephyra elegans.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License