SciELO - Scientific Electronic Library Online

 
vol.58 número1THE EFFECT OF DIFFERENT SUBSTRATES ON TRITERPENOIDS AND FATTY ACIDS IN FUNGI OF THE GENUS PleurotusENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Journal of the Chilean Chemical Society

versión On-line ISSN 0717-9707

Resumen

SULTANA, NAJMA; ARAYNE, MUHAMMAD SAEED; NAWAZ, MUHAMMAD  y  AMIR ALI, KIRAN. ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS. J. Chil. Chem. Soc. [online]. 2013, vol.58, n.1, pp.1584-1589. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072013000100018.

In this work, two methods are described for the simultaneous determination of clopidogrel bisulfate with HMG Co-A reductase inhibitors/statins. The first method comprised of quantitative determinations of clopidogrel bisulfate in presence of HMG Co-A reductase inhibitors (atorvastatin, pravastatin simvastatin and rosuvastatin) in buffers of pH 1, 4 (simulating gastric environments), pH 7.4 (simulating blood pH) and pH 9 (simulating intestinal pH) at body temperature (37oC) using UV/VIS spectrophotometer. Derivative spectroscopy coupled with zero-crossing measurements was used for separating the interfering wavelengths of clopidogrel and statins. Least square regression analysis was performed to measure the concentrations of these drugs. It was observed that clopidogrel interacted with statins at all four pH, especially in simulated gastric environments. In the second case, reversed phase liquid chromatographic (RP-LC) method was developed for simultaneous determination and to investigate the interactions between clopidogrel and HMG Co-A reductase inhibitors. These interactions were evaluated by RP-LC in buffers of pH 1, 4, 7.4 and 9. These studies further supported the evidence of complexation between clopidogrel and statins as the area under the curve (AUC) was found increased in almost all four pH solutions.

Palabras clave : Clopidogrel; Statins; Interactions; UV-Vis; RP-HPLC.

        · texto en Inglés     · Inglés ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons