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International Journal of Morphology

On-line version ISSN 0717-9502


JUNQUEIRA, Rafael Binato et al. In vitro Analysis of Morphology of Human Enamel Submitted to Excessive Use of External Bleaching Agents. Int. J. Morphol. [online]. 2011, vol.29, n.1, pp.118-122. ISSN 0717-9502.

The aim of this study was to evaluate the effects of external bleaching agents on morphology of human enamel after excessive homemade dental bleaching. 20 intact human third molars were submitted to mesio-distal crosscut and embedded in polystyrene resin. The specimens were submitted to finish and a half of enamel surface of each specimen was covered with cosmetic varnish, meaning control group (G0). The specimens were randomly divided into four groups (n=10): G1 ­ 1 bleaching session by 16% carbamide peroxide; G2 ­ 3 bleaching sessions by 16% carbamide peroxide; G3 ­ 1 bleaching session by 22% carbamide peroxide; G4 ­ 3 bleaching sessions by 22% carbamide peroxide. Each session lasted 8 hours a day, during two weeks, with 45 days of interval between sessions. In this period, the specimens were kept in artificial saliva at 37°C. The specimens were observed by scanning electron microscopy and the results showed depression areas, irregularities, erosion, and enamel prisms exposition, which was more evident in G4. It can be concluded that the excess of bleaching produced many alterations on enamel surface, mainly when was used carbamide peroxide in higher concentration.

Keywords : Dental bleaching; Dental enamel; Peroxides.

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