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International Journal of Morphology

On-line version ISSN 0717-9502


AVWIORO, O. G. et al. Studies on the Effects of Fixatives on the Staining Ability of Morinda lucida Extracts on Tissue Sections. Int. J. Morphol. [online]. 2007, vol.25, n.2, pp.335-336. ISSN 0717-9502.

The objective of the study was to determine the best fixative for tissue sections, which were to be stained with the dye extracts oí Morinda lucida, a stain for collagen fibres and muscle fibres. The effects of 10% formalin saline, Carnoy's fluid, Zenker's fluid, Helly's fluid and Bouin's fluid on the staining ability of the dye extracted from Morinda lucida on tissue sections were studied. Human tissues were fixed in the afore mentioned fixatives and the sections stained with acidified alcoholic solution of Morinda lucida extract. No visible difference in the staining reactions was observed when the stained slides from the different fixatives were compared. The colour, staining time and staining intensity were the same in all the sections studied

Keywords : Formalin saline; Carnoy's fluid; Zenker's fluid; Helly's fluid and Bouin's fluid.

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