SciELO - Scientific Electronic Library Online

 
vol.58 número1-2Comunidades ectoparasitarias branquiales de la pintadilla Cheilodactylus variegatus Valenciennes 1833 (Pisces: Cheilodactylidae)Primer registro de Amblyomma longirostre (Acari: Ixodidae) en Uruguay índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Parasitología latinoamericana

versión On-line ISSN 0717-7712

Resumen

ECHENIQUE, CLAUDIA GABRIELA  y  MAGARO, HORTENSIA MARÍA. ADHERENCE OF Trichomonas muris IN VITRO. Parasitol. latinoam. [online]. 2003, vol.58, n.1-2, pp.68-71. ISSN 0717-7712.  http://dx.doi.org/10.4067/S0717-77122003000100011.

Microorganisms adherence depends on the microorganism, on the epithelial cells and on environmental factors. The objetive was to study the action of metronidazole, CO2 atmosphere and pH on the adherence of Trichomonas muris to buccal epithelial cells. The epithelial cells were obtained by hysopping the inner faces of a healthy donor's cheeks. The cells were placed with 1,5 ml of PBS (100 cells / ml). T. muris obtained from the faecal matter of adult Rockland mice, were cultured in TYI - S -33 medium supplemented with bile and antibiotics, having a replication every 48 hours. Gibbons and van Houte technique was employed with some modifications. T. muris adhered to 100 epithelial cells, were counted. The assay was carried out with 0,1 mg metronidazole and added to PBS in a CO2 atmosphere, incubating the tubes at 37º C during 60 minutes with the following pH values: 3,5,7 and 10. 10% of T. muris adhered with metronidazole and 7% with CO2 . At pH 3 they were dead, to pH 5 and 7, 25% adhered and to pH 10, adhered 13%. At pH 7, 37ºC , in CO2 atmosphere, 27% of T. muris adhered to epithelial cells

Palabras clave : Trichomonas muris; protozoan; metronidazole.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons