SciELO - Scientific Electronic Library Online

 
vol.24 número1Test de marcha de seis minutos en niños con bronquiolitis obliterante postviral: Correlación con espirometría índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Revista chilena de enfermedades respiratorias

versión On-line ISSN 0717-7348

Resumen

VECCHIOLA C, ANDREA et al. Intratracheal instillation of elastase in the rat: Electrophoretical changes of alphal-antitrypsin in bronchoalveolar lavage fluid. Rev. chil. enferm. respir. [online]. 2008, vol.24, n.1, pp.7-14. ISSN 0717-7348.  http://dx.doi.org/10.4067/S0717-73482008000100002.

Introduction: Endogenous alphal-antitrypsin ( á1-AT) is the main inhibitor of the intratracheally instilled elastase in experimental animals. Objective: To evaluate by electrophoresis and immunodetection using western blot analysis, the different forms of á1-AT in bronchoalveolar lavage fluid (BALF) of Sprague Dawley rats after intratracheal instillation of elastase, with the hypothesis that the previously observed increment in antielastase activity is due to high levels of active á1-AT. Results: In the first hours after elastase instillation the concentration of á1-AT increases more than seven times due to an increase in alveolar-capillary permeability. á1-AT in BAIF is found as the native protein (~ 52 kDa), as complexes of different molecular sizes (> 75 kDa and > 100 kDa) and as a proteolytic product (< 40 kDa). Conclusion: In spite of a high proportion of á1-AT in the inactive form as part of different complexes, the increase in alveolar-capillary permeability after elastase treatment contributes to maintain high levels of active á1-AT. These results could be of importance in other inflammatory lung processes.

Palabras clave : antiprotease; á1-AT; diffuse lung damage; elastase; electrophoresis.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons