Abstract

FERRADA, Sandra; HERNANDEZ, Klaudia; MONTOYA, R.  and  GALLEGUILLOS, Ricardo. POPULATION STUDY OF THE ANCHOVY RESOURCE (ENGRAULIS RINGENS JENYNS 1842) (CLUPEIFORMES, ENGRAULIDAE), THROUGH DNA ANALYSIS. Gayana (Concepc.) [online]. 2002, vol.66, n.2, pp.243-248. ISSN 0717-652X.  http://dx.doi.org/10.4067/S0717-65382002000200022.

The genetic variability of two nuclear genes, the 4/5 intron of the Calmodulin (CaM) and the ITS (Internal Transcribed Spacers) of the DNAr were studied. The methodology applied in 139 anchovies (E. ringens) from Iquique and Talcahuano, were the PCR amplification for CaM samples and the PCR amplification and digestion, for ITS samples. The results exhibited 8 genotypes for CaM in Talcahuano, and 7 were identified in Iquique. The size of the fragments founded (1-3), ranged from 550 to 1100 bp. The ITS amplification and digestion of a fragment determined the existence of 3 genotypes, 2 in Talcahuano and 3 in Iquique. But in the digestion of the ITS amplifications, only the Taq^aI endonuclease showed diferences in the restriction profiles. The genotypic frequencies are discussed for each gene and study area, as well as the presence of only one stock from a genetic point of view

Keywords : Engraulis ringens; ITS; CaM; genetic variability; stock.