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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


WANG, Chuan Tang et al. Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut. Electron. J. Biotechnol. [online]. 2010, vol.13, n.5, pp.18-19. ISSN 0717-3458.

A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.

Palabras clave : identification; NIRS; PCR; peanut; true hybrid.

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