SciELO - Scientific Electronic Library Online

vol.9 número2Production of polyclonal antisera to manganese superoxide dismutase expressed in downy mildew resistant pearl millet and its application for immunodiagnosisRecovery of lead and cadmium ions from metal-loaded biomass of wild cocoyam (Caladium bicolor) using acidic, basic and neutral eluent solutions índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


ARENAS, Gloria et al. Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.. Electron. J. Biotechnol. [online]. 2006, vol.9, n.2. ISSN 0717-3458.

A "defensin-like" antibacterial peptide from Mytilus edulis chilensis, was sub-cloned into a binary vector for expression in plant tissues. The resulting new clone was electroporated into A. tumefaciens to transform tobacco plants. The presence of the construct in transgenic tobacco lines was demonstrated through RT-PCR, Northern and Western blots. Transformed positive plants were selected and grown for challenging. Tobacco leaves were infiltrated with Pseudomonassyringae pv. syringae and visual lesions determined at different times post-exposure. Of seven plants exposed, four gave variable protection up to seven days post-infection while one of them appears to be fully protected. These results suggest that defensin-like antimicrobial peptides from molluscs are a good source to provide resistance of tobacco plants to Pseudomonassyringae pv. syringae.

Palabras clave : antibacterial peptide; disease resistance; in vivo expression; Nicotiana tabacum L.; Pseudomonassyringae pv. syringae; transgenic tobacco plants.

        · texto en Inglés     · Inglés ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons