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vol.19 issue1DOMINANCE RELATIONS IN THE LIMBIC LOBEMORPHOLOGICAL STUDY OF THE FEMALE EXTERNAL GENITALIA OF THE MYOCASTOR COYPUS (COYPU) author indexsubject indexarticles search
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Revista chilena de anatomía

Print version ISSN 0716-9868

Abstract

DALLARD, Bibiana. E. et al. LECTINHISTOCHEMICAL AND MORPHOLOGICAL STUDY OF LLAMA (Lama glama) MAMMARY GLAND DURING LACTATION. Rev. chil. anat. [online]. 2001, vol.19, n.1, pp.11-16. ISSN 0716-9868.  http://dx.doi.org/10.4067/S0716-98682001000100002.

Oligosacarids expression in llama (Lama glama) mammary glands was evaluated during lactation period by means of lectins. The lactocite nuclear diameter, alveoli mean diameter and the percentage of the area occupiedby alveolar lumen were compared by morphometric analysis. Fifteen females were biopsed on days 5, 15, 30, 60 y 120 after freshening. Seven biotynilated lectines (Con-A, WGA, DBA, SBA, PNA, RCA-I, UEA-I) were used, following the pre-established protocols (ABC Method). Apical domain cytoplasm of lactocites reacted intensely with lectins WGA and RCA; and moderately with Con-A during the whole lactation period. Lactocites glycocalyx reacted intensely with SBA y PNA in every studied period; fromday 5 to day 15 the reaction was remarkable for RCA, and from day 15 to 120 days of lactation, it was so for DBA. There was not marking of the parenchyma with UEA-I during the whole assay length. The results showed that carbohydrates express themselves in different ways as the lactation progresses, which indicates the occurrence of bio-molecular changes due to intense functional activity. Morphometrical analysis revealed that there is not variation in the relative area occupied by alveoli, but it does exist a significative increase (p<0,05) in the alveolus size since day 15, together with a decrease in the lactocites nuclear  size beginning on day 60

Keywords : Llamas; Mammary gland; Lectins; Morphometry.

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