SciELO - Scientific Electronic Library Online

vol.39 número1Regulation of transepithelial transport of iron by hepcidinCharacterization of mitochondrial iron uptake in HepG2 cells índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Biological Research

versión impresa ISSN 0716-9760


MENDIBURO, MARÍA JOSE; FLORES, SEBASTIÁN; PIZARRO, FERNANDO  y  ARREDONDO, MIGUEL. Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells. Biol. Res. [online]. 2006, vol.39, n.1, pp.195-197. ISSN 0716-9760.

Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin or released by an iron exporter. The mechanism by which heme enters cells is not completely understood, although it had been suggested that it might be internalized by an endocytosis process. In this study, we expressed a full-length Heme oxygenase-1 cDNA in Caco-2 cells and measured intracellular iron content, heme-iron uptake and transport and immunolocalization of heme oxygenase-1 in these cells. We found that heme oxygenase-1 expressing cells showed increased apical heme iron uptake and transepithelial transport when compared to control cells. These results suggested that heme oxygenase-1 mediates heme iron influx and efflux in intestinal cells.

Palabras clave : iron; heme; heme oxygenase; Caco-2 cells.

        · texto en Inglés     · Inglés ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons