SciELO - Scientific Electronic Library Online

 
vol.34 número3-4Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitroStructure analysis of the endoxylanase A gene from Penicillium purpurogenum índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Biological Research

versión impresa ISSN 0716-9760

Resumen

ARAYA, PAMELA; ROSEMBLATT, MARIO; VALENZUELA, PABLO  y  MURIALDO, HELIOS. The Bacteriophage lDNA packaging enzyme: Identification of four structural domains of the gpNu1 subunit using limited proteolysis. Biol. Res. [online]. 2001, vol.34, n.3-4, pp.207-216. ISSN 0716-9760.  http://dx.doi.org/10.4067/S0716-97602001000300008.

Lambda DNA terminase, the enzyme that cleaves virion-length chromosomes from multigenomic concatemers and packages them into the bacteriophage head, is composed of two subunits, gpNu1 and gpA. Direct determination of the structure of gpNu1, the smaller subunit, has not been possible because of its insolubility in aqueous solutions. Therefore, to identify smaller and potentially water-soluble domains of gpNu1, we analyzed the nature of the products obtained by limited digestion of the protein with several proteases. The gpNu1 subunit was obtained from E.coli cells transfected with the plasmid pH6-Nu1 that overproduces the protein. Incubation of gpNu1 solubized in 2.5 M guanidinium chloride with chymotrypsin resulted in the formation of at least eight discrete protein bands, while treatment with endoproteinase glu-C and bromelain yielded three and one major bands, respectively. The peptides generated by digestion with the various proteases were separated by two-dimensional gel electrophoresis and transferred to Immobilon membranes. Amino acid sequencing of the peptides allowed for the precise assignment of their N-terminal amino acid, while their estimated molecular weights permitted the identification of their C-terminal ends. The results reveal that in the presence of 2.5 M guanidinium chloride, gpNu1 is partially folded in at least four distinct structural domains that correspond to functional domains as determined by previously reported genetic experiments. This information is key to design new plasmids to overproduce these domains for further structural analysis.

Palabras clave : DNA packaging; terminase; gpNu1 structural domains; limited proteolysis.

        · texto en Inglés

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons