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Revista chilena de infectología

Print version ISSN 0716-1018

Abstract

MELO A, Angélica et al. Human papillomavirus genotyping in cervical adenocarcinoma in the Region of La Araucanía-Chile. Rev. chil. infectol. [online]. 2010, vol.27, n.4, pp.297-301. ISSN 0716-1018.  http://dx.doi.org/10.4067/S0716-10182010000500001.

Human papillomavirus (HPV) is the main cause of cervical cancer. Thus, HPV detection and typing becomes important in order to know the frequency of genotypes present in the region. In this paper we studied 44 biopsies of cervical adenocarcinoma. For HPV detection nested polymerase chain reaction (PCR) was used to amplify the L1 gene. For viral typing restriction enzymes (Rsa I, Dde I, Pst I) and DNA sequencing were used. Viral DNA was detected by nested L1 PCR in 100% of biopsies; 38/44 cases could be typed: 81.6% HPV16; 13.2% HPV 18; 2.6% VPH 33 and 2.6% HPV 18/33. Conclusions: The technique was successful in identifying the virus type in 86% of biopsies. There was a strong association ACC-HPV, especially with the viral type 16, detected in 81.6% of established cases.

Keywords : HPV; cervical cancer; cervical adenocarcinoma; PCR; RFLP; Sequence.

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