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vol.63 número2CARACTERIZACIÓN PRELIMINAR DE UNA COLECCIÓN DE LEGUMINOSAS FORRAJERAS ANUALES PARA LA ZONA MEDITERRANEA DE CHILEMANEJO DE RIEGO POR GOTEO EN UVA DE MESA CV. THOMPSON SEEDLESS CULTIVADA EN SUELOS DE TEXTURA FINA índice de autoresíndice de materiabúsqueda de artículos
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Agricultura Técnica

versión impresa ISSN 0365-2807

Resumen

CAMPILLO R., Ricardo; URQUIAGA C., Segundo; PINO N., Inés  y  MONTENEGRO B., Adolfo. Estimation of biological nitrogen fixation in forage legumes using a 15N labeling methodology. Agric. Téc. [online]. 2003, vol.63, n.2, pp.169-179. ISSN 0365-2807.  http://dx.doi.org/10.4067/S0365-28072003000200006.

The contribution of biological nitrogen N2 fixation (FBN) in four forage legumes: white clover (Trifolium repens L.), red clover (Trifolium pratense L.), subterranean clover (Trifolium subterraneum L.) and alfalfa (Medicago sativa L.), with and without specific rhizobium inoculation, was evaluated by the A value isotope technique, using three grasses: oats (Avena sativa L.), perennial ryegrass (Lolium perenne L.) and fescue (Festuca arundinacea Schreb.) as reference crops. The study was carried out for three years on an andisol (Typic fulvudand), at the Carillanca Regional Research Center of de National Agricultural Research Institute, IX Region, Chile. The dose of N applied was 10 kg N ha-1 using ammonium sulfate (10 atom % 15N excess) for legume species, and 30 kg ha-1 as ammonium sulfate (5 atom % 15N excess) for grass species. Plant samples were analyzed for total N (Kjeldahl) and atom % 15N excess (optical emission spectrometry). All the three reference crops showed similar values of FBN to the legumes. The FBN capacity reached 90% in all seasons and by all the legumes. No significant influence of inoculation with rhizobium was found, except for alfalfa and only in the first season. The quantity of N fixed in all seasons was high and for all the legumes, ranging from 60 to 770 kg ha-1 year-1, for subterranean clover and alfalfa, respectively, confirming the great efficiency of the FBN mechanism on the forage legumes under study.

Palabras clave : forage legumes; inoculation; nitrogen; isotopic techniques.

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