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vol.61 número1TOXICIDAD DE PESTICIDAS SOBRE ENEMIGOS NATURALES DE PLAGAS AGRICOLASEFECTO DEL AGUA Y FERTIRRIGACION EN EL DESARROLLO Y PRODUCCIÓN DE NARANJOS CV. THOMPSON NAVEL índice de autoresíndice de materiabúsqueda de artículos
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Agricultura Técnica

versión impresa ISSN 0365-2807

Resumen

FELMER D., Ricardo; BUTENDIECK B., Norberto; BUTENDIECK B., Bárbara  y  VILLEGAS M., Juana. Detection of a bovine genetic defect by a DNA probe. Agric. Téc. [online]. 2001, vol.61, n.1, pp.42-50. ISSN 0365-2807.  http://dx.doi.org/10.4067/S0365-28072001000100005.

The adhesion deficiency of bovine leukocytes to antigens, known as BLAD (bovine leukocyte adhesion deficiency), is a hereditary genetic disease which is lethal for the Holstein breed. It is a recessive autosomal disease that can be transmitted to the offspring. The objective of this research was to standardize the molecular techniques to diagnose BLAD and also to get a first approximation of the genetic frequency of the mutated allele in the bull population of the IXth and Xth Regions. DNA amplification using polymerase chain reaction (PCR) and posterior digestion with restriction enzymes Taq I and Hae III was performed. The digested product was analyzed by 4% agarose gel electrophoresis and ethidium bromide staining to show the typical restriction fragments present in normal, carrier and infected cattle. The technique was validated with a screening test of 59 cattle. Out of 55 bulls analyzed, one turned out to be a BLAD carrier, which suggests a genetic frequency of 1.79% in the bull population of the IXth and Xth Regions. The diagnostic technique of PCR and the digestion of the amplification product with two restriction enzymes (Taq I and Hae III) has identified with accuracy the genotype of cattle at a specific locus and allows an early identification of the genetic defect known as BLAD in infected and carrier animals.

Palabras clave : bovine leukocyte adhesion deficiency; BLAD; PCR; Holstein breed; RFLP; disease.

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