SciELO - Scientific Electronic Library Online

 
vol.47 número2Distribución, características epidemiológicas y métodos de control del patógeno Nosema ceranae Fries en abejas Apis mellifera L. (Hymenoptera, Apidae)Evaluación productiva de un programa de inyección sin aguja para la administración de vacunas contra Circovirus porcino tipo 2 y Mycoplasma hyopneumoniae en un plantel porcino intensivo tipo destete-venta índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Archivos de medicina veterinaria

versión impresa ISSN 0301-732X

Resumen

LEYAN, V; CHIHUAILAF, R  y  ORTEGA, M. Humoral and cellular immune response to Brucella abortus strain RB51 vaccine in grazing heifers supplemented with selenium and α-tocopherol. Arch. med. vet. [online]. 2015, vol.47, n.2, pp.139-145. ISSN 0301-732X.  http://dx.doi.org/10.4067/S0301-732X2015000200003.

In order to evaluate the effect of selenium and α-tocopherol supplementation on the immune response to Brucella abortus strain RB51, four groups of six grazing heifers were used. Three months before the start of the trial, the groups Se-T and Se were supplemented in single dose with barium selenate (1 mg selenium/kg body weigth) and groups Se-T and T were supplemented with 500 IU α-tocopherol/100 kg each two months. Group C was maintained without supplementation. Once established differences in metabolic balance groups, the trial began with the RB51 vaccine administration. Blood samples with heparin and without additive were obtained from each cow for the determining blood activity of glutathione peroxidase (GPx) and plasma concentration of cholesterol and α-tocopherol. The humoral immune response was evaluated by ELISA and the cellular immune response by an intradermal test antigen of B. abortus. Differences between groups were evaluated by tests repeated measures ANOVA, paired samples t and Kruskal-Wallis. It was considered significant when the P value was less than 0.05. Supplementation with selenium and α-tocopherol established differences between groups (P < 0.05). The humoral immune response and cellular response to RB51 vaccine was lower in group T (P < 0.05). It is possible to conclude that humoral and cellular immune response to the vaccine RB51 can be negatively modulated by plasma α-tocopherol concentration at the immunization.

Palabras clave : immune response; selenium; α-tocopherol; Brucella abortus.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons