Scielo RSS <![CDATA[Parasitología latinoamericana]]> http://www.scielo.cl/rss.php?pid=0717-771220030001&lang=es vol. 58 num. 1-2 lang. es <![CDATA[SciELO Logo]]> http://www.scielo.cl/img/en/fbpelogp.gif http://www.scielo.cl <![CDATA[<I>Variaciones biológicas de Trypanosoma cruzi (Kinetoplastida:Trypanosomatidae) asociadas con la ingestión de diferentes tipos de sangre por el vector Triatoma dimidiata (Hemiptera: Reduviidae)</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100001&lng=es&nrm=iso&tlng=es The effect of avian and mouse blood in the gut of Triatoma dimidiata (Hemiptera: Reduviidae) on the biological characteristics of two costarican strains of T. cruzi (TC-2 and TC-4) was evaluated in a murine model. This relationship defined four experimental systems named TC-2 gallina, TC-2 raton, TC-4 gallina and TC-4 raton. Parasites from each system were inoculated intraperitonealy in C3H mice and the course of the infections were observed three times a week during 60 days. The observations included parasitemia levels (parasites/mm³), concentration of slender and broad forms, duration of prepatent and patent periods, survival, and cumulated mortality. The infection characteristics in all the systems were similar with parasitemia peaks in the days 23 to 33 post infection and broad forms as the predominant tripomastigote circulating forms in all the systems. Some variables such as prepatent and patent periods did not show statistically significant differences (p < 0.05). However, the survival was prolonged in mice that were infected to parasites associated to avian blood. The evolution of the mortality was slower in these systems. The data suggests that the parasite virulence modulation could be related to the particular blood sources in the vectors<hr/>El efecto de las sangres aviar y murina sobre las características biológicas de dos cepas costa-rricenses de T. cruzi (TC-2 y TC-4) fue evaluado mediante un modelo experimental en el cual se infectaron ratones con parásitos previamente expuestos a estos tipos de sangre en el intestino del vector Triatoma dimidiata (Hemiptera: Reduviidae). La relación que se estableció entre las cepas y los tipos de sangre utilizados permitió definir cuatro sistemas experimentales denomi-nados arbitrariamente TC-2 gallina, TC-ratón, TC-4 gallina y TC-4 ratón. Parásitos de cada sistema fueron inoculados intraperitonealmente en ratones C3H y el progreso de la infección fue registrado tres veces por semana durante un período de 60 días. Las observaciones incluyeron: niveles de parasitemia (parásitos/mm³), concentración de formas gruesas y delgadas, duración de los períodos prepatente, patente y de supervivencia y la mortalidad acumulada. Las características de las infecciones en todos los sistemas fueron similares. Estas mostraron picos de parasitemia entre los días 23 y 33 post infección y las formas gruesas fueron los tripomastigotos san-guíneos predominantes. Algunas variables como los períodos prepatente y patente no mostraron diferencias es significativas (p > 0,05). Sin embargo, el período de supervivencia se vio significa-tivamente prolongado en los ratones infectados con parásitos asociados a sangre aviar (p < 0,05). La evolución de la mortalidad fue menos marcada en estos sistemas. Los datos sugieren la ocurrencia de una modulación en las virulencia parasitaria que podría estar relacionada con el tipo de sangre presente en el insecto vector <![CDATA[<I>Aproximación a una caracterización molecular de Fasciola hepatica por la técnica RAPDs - PCR</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100002&lng=es&nrm=iso&tlng=es The present study show the molecular characterization of Fasciola hepatica taken from cows, horses and sheeps, using the Random Amplified Polymorphic ADN Fragments (RAPDs-PCR) technique. The standardization of the optimal conditions of amplification and thermocyclation for F. hepatica by RAPDs-PCR were made, as the genetic markers for polymorphic identification of the parasites collected from different animals specie.The methodology used compared the genetic pattern between species and inside each specie. The results shows random genetic markers, given genetic variations of F. hepatica between species and inside each specie (polymorphism), and the amplifications fragments were between 135 and 741 pair of bases (bp)<hr/>El presente estudio muestra la caracterización molecular de Fasciola hepatica obtenidas de bovino, equino y ovino, utilizando la técnica de Amplificación al Azar de Fragmentos de ADN Polimórficos (RAPDs-PCR). Para este fin, se lograron estandarizar las condiciones óptimas de amplificación y programa de termociclación de RAPDs-PCR para F. hepatica, así como marcadores genéticos de identificación poli-mórfica características para cada especie. La metodología utilizada consideró comparar los patrones genéticos interespecie e intraespecie, a partir de muestras de F. hepatica. Los resultados obtenidos muestran marcadores genéticos al azar, que evidencian variabilidad genética de F. hepatica intra e interespecie (polimorfismo), y cuyos fragmentos de amplificación fluctuaron entre los 135 y 741 pares de bases (pb) <![CDATA[<I>Encuesta coproparasitológico canina realizado en plazas publicas de la ciudad de Mar del Plata, Buenos Aires, Argentina</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100003&lng=es&nrm=iso&tlng=es In Mar del Plata city, Buenos Aires province (Argentina), a sampling of canine faeces was made at 11 public squares with the aim to identify zoonotic parasite species from dogs. Squares were classified into two groups: Central and Peripheric. Samples were taken at square flowerbeds (90.4%), special cages for dogs (6.4%) and child´s games areas (3.2%). All the squares were found contaminated with faeces and 100% of them were positive for parasites. Total prevalence was 49.95%; prevalence by parasite species was 62.96% for Ancylostoma caninum and Trichuris vulpis, 24.07% for Uncinaria stenocephala, 22.22% for Toxocara canis, 9.25% for Amoeba spp., 3.70% for coccidians and 1.85% for Pseudophyllideans. Percentage of parasitized samples was higher at peripheric squares than at the central group. Parasite prevalences at each square group were compared with results registered in different areas of Argentina. Results obtained in the present study, as related with canine faeces disseminated along public places and the sanitary consequences to the human health, are discussed<hr/>En la ciudad de Mar del Plata, provincia de Buenos Aires (Argentina) se realizó un muestreo de 11 plazas públicas, con el propósito de identificar las especies de parásitos presentes en la materia fecal canina, en especial aquellos de importancia zoonótica. Las plazas fueron clasificadas en céntricas y periféricas. Se extrajeron muestras en canteros (90,4%), caniles (6,4%) y lugares de juegos (3,2%) de cada una de las plazas y se efectuó el análisis coproparasitológico. Todas las plazas presentaron contaminación con materia fecal y el 100% resultó positivo a la presencia de parásitos. La prevalencia total de parásitos para el conjunto de plazas fue del 49,95% y las prevalencias por especie de parásito fueron: Ancylostoma caninum y Trichuris vulpis (62,96%), Uncinaria stenocephala (24,07%), Toxocara canis (22,22%), Amoeba spp. (9,25%), coccidios (3,70%) y Pseudophyllideos (1,85%). El porcentaje de muestras parasitadas fue mayor en las plazas de la periferia que en el grupo del centro. Las prevalencias registradas en cada uno de los grupos de plazas se compararon con las calculadas en otras áreas geográficas de la República Argentina <![CDATA[ANTHELMINTIC ACTION OF THE CRUDE EXTRACTS OF <I>Andira anthelmia </I>(VELL.) MACBR. AND <I>Andira fraxinifolia</I> BENTH., IN MICE NATURALLY INFECTED BY <I> Vampirolepis nana </I>AND <I>Aspiculuris tetraptera</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100004&lng=es&nrm=iso&tlng=es The anthelmintic activity of the crude extracts of fresh and dry leaf, trunk bark and root bark of the species Andira anthelmia and Andira fraxinifolia. was evaluated in the species Vampirolepis nana and Aspiculuris tetraptera. Doses of 8 g/kg/day were utilized for the leaves and trunk bark, and 1, 2, 4 and 8 g/kg/day for the root bark for the A. anthelmia, and doses of 8 g/kg/day were utilized for leaves and root bark, and of 4, 8 and 16 g/kg/day for tree bark, for the A. fraxinifolia. The dose of 8 g/kg/day of the crude extract of the fresh and dry leaf, the trunk bark and root bark, of the A. anthelmia, when compared, did not present any significant difference in the percentage elimination of V. nana, 14.9%, 16.4%, 6.3%, 17.9%, respectively. The same occurred when compared with the control group (7.4%). The doses of 2 and 4 g/kg/day, 49.4% and 42.7, respectively, were significantly more active for V. nana, in comparison with the other anatomical parts and the control group, and did not present any difference in relation to the mebendazole. The 2 g/kg/day dose was significantly more active for A. tetraptera (63.2%), in comparison with the leaves, with the root bark for the doses of 1 and 4 g/kg/day, and with the control group, 1.2%, 1.7%, 31.7%, 15.3%, 0.1%, respectively. However, it did not present any significant difference in relation to nitroscanate, this being less active than mebendazole. Although the 8 g/kg/day dose for the tree bark presented a higher percentage elimination of V. nana (37.5%), no statistical difference was observed, when compared with the other doses, anatomical parts, the control group and the mebendazole. Similarly, no significant difference was observed in the percentage elimination of A. tetraptera. The results suggested the crude extract of the root bark of the A. anthelmia as a promising anthelmintic, requiring further studies, due to the high toxicity presented<hr/>A atividade anti-helmíntica dos extratos brutos da folha fresca e seca, casca do tronco e da raiz da espécie Andira anthelmia e A. fraxinifolia, foi avaliada sob as espécies Vampirolepis nana e Aspiculuris tetraptera. Foram utilizadas as doses de 8 g/kg/dia para folhas e casca do tronco, e de 1, 2, 4 e 8 g/kg/dia para a casca da raiz de A. anthelmia, e as doses de 8 g/kg/dia para folhas e casca da raiz, e de 4, 8 e 16 g/kg/dia para a casca do tronco de A. fraxinifolia. A dose de 8 g/kg/dia do extrato bruto da folha fresca e seca, da casca do tronco e da raiz, quando comparadas, não apresentaram diferença significativa no percentual de eliminação de V. nana, 14,9%, 16,4%, 6,3%, 17,9%, respectivamente, o mesmo ocorreu quando comparadas ao grupo controle (7,4%). As doses de 2 e 4 g/kg/dia, 49,4% e 42,7%, respectivamente, foram significativa-mente mais ativas para V. nana, em comparação com as demais partes anatômicas e o grupo controle, não apresentando diferença em relação a dose de 1 g/kg/dia da casca da raiz e do mebendazol. A dose 2 g/kg/dia foi significati-vamente mais ativa para A. tetraptera (63,2%), em comparação com as folhas, a casca da raiz nas doses de 1 e 4 g/kg/dia, e o grupo controle, 1,2%, 1,7%, 31,7%, 15,3%, 0,1%, respec-tivamente. Contudo, não apresentou diferença significativa em relação ao nitroscanato, sendo menos ativa do que o mebendazol. Embora a dose de 8 g/kg/dia da casca do tronco de A. fraxinifolia tenha apresentado um percentual de eliminação de V. nana maior (37,5%), não foi observado diferença estatística, quando comparado com as demais doses, partes anatômicas, grupo controle e o mebendazol. Similarmente, não foi observado diferença significativa nos percentuais de eliminação de A. tetraptera. Os resultados sugerem o extrato bruto da casca da raiz de A. anthelmia como anti-helmíntico promissor, necessitando de estudos complementares, devido a alta toxicidade apresentada <![CDATA[<I>Enteroparasitosis en poblaciones indígenas y mestizas de la Sierra de Nayarit, México</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100005&lng=es&nrm=iso&tlng=es We compared the prevalences of enteroparasitoses from 420 individuals, 306 of them were indigenous and 114 were mestizo persons, without gender differentiation, and of all ages from the Nayarit mountain range. Six field trips were made to the region during one year to collect the stool samples, some individuals provided only one sample and other even five. Stool samples were suspended 1:5 with 10% formalin for their transportation to Mexico City, where they were processed by direct parasitoscopic analysis with lugol solution We found Entamoeba histolytica, 59.8%; Giardia lamblia, 22.2%; Enterobius vermicularis, 22.2%; Hymenolepis nana, 15.4%; 2 cases of taeniosis (0.7%), 21 of ascariosis (6.9%), 2 of strongyloidosis (0.7%) and 7 of trichuriosis (2.3%) among the huichol population. For the mestizo population, we found 43.9% of entamoebosis, 14.0% giardiosis, 9.6% hymenolepiosis, 5 cases of ascariosis, 2 of taeniosis, 1 of strongyloidosis and 1 of enterobiosis. Chi-square test revealed statistically significant differences between the two populations for some of the calculated prevalences<hr/>Comparamos las prevalencias de enteropa-rasitosis a partir de 420 individuos, 306 de ellos eran indígenas y 114 eran mestizos, sin dife-renciación de género, y de todos los rangos de edad, de la Sierra de Nayarit. Se hicieron seis viajes al campo a la región durante un año para recoger muestras de materia fecal, algunos individuos proporcionaron solamente una muestra y otros cinco muestras. Las muestras de materia fecal fueron diluidas en una solución de formalina al 10% en una relación 1:10, para su conservación durante su transporte a la Ciudad de México, donde se procesaron por análisis coproparasitoscópico directo teñido con solución de lugol. Nosotros encontramos 59,8% de Entamoeba histolytica, 22,2% de Giardia lamblia, 22,2% de Enterobius vermicularis, 15,4% de Hymenolepis nana, 2 casos de taeniosis (0,7%), 21 casos de ascariosis (6,9%), 2 casos de estrongyloidosis (0,7%) y 7 casos de trichuriosis (2,3%) entre la población huichol. En la población mestiza, encontramos 43,9% de entamoebosis, 14,0% de giardiosis 9,6% de hymenolepiosis, 5 casos de ascariosis, 2 de taeniosis, 1 de strongyloidosis y 1 de enterobiosis. Los resultados se analizaron por la prueba del Chi-cuadrada y revelaron diferencias estadísticas significativas entre las dos poblaciones estudiadas <![CDATA[<I>Parasitosis intestinal en poblaciones urbana y rural en Sandia, Departamento de Puno, Perú</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100006&lng=es&nrm=iso&tlng=es The objective of the present,study, is to determine the rate of intestinal parasitoses in rural and urban populations of Sandia district, located at 2178 msnm., department of Puno, Perú. Fecal samples, 35 from rural (Group A) and 37 from urban (Group B) populations were collected (total 72). Four parasitological tests were used: Direct Microscopy, Kato Technique, Spontaneous Tube Sedimentation Technique and Modified Cup Baermann Technique. Global prevalences of intestinal parasitoses in both groups were high (A: 88.58%; B: 67.57%). Prevalence of intestinal parasitoses was: Ascaris lumbricoides (A: 51.42%; B: 29.72%, p = 0.06), Entamoeba coli (A: 48.57%; B: 18.91%, p = 0.007), Trichuris trichiura (A: 42.85%; B: 13.51%, p = 0.005), Blastocystis hominis (A: 40%; B: 32.42%, p = 0.5), Endolimax nana (A: 37.14%; B: 16.66%, p = 0.04), Giardia lamblia (A: 25.71%; B: 13.51%, p = 0.19), Hymenolepis nana (A: 0%; B: 5.4%), Iodamoeba butschli (A: 2.85%; B: 0%), Entamoeba histolytica / E dispar (A: 2.85%; B: 0%), Strngyloides stercoralis (A: 2.85%; B: 0%) and Ancylostoma duodenale / Necator americanus (A: 0%; B: 2.7%). We conclude that intestinal parasitism is more frequent in rural than urban populations, is associated to poverty, poorer environmental sanitation and lack of hygienic services. Due to the high rates of parasitic infection we found, it is important to perform adequate parasitological tests to allow optimal diagnosis and opportune and effective treatment<hr/>El objetivo del presente estudio de tipo descriptivo y de corte transversal, es determinar la tasa de enteroparasitismo en las poblaciones rural y urbana del distrito de Sandia, localizado a 2.178 msnm, departamento de Puno, Perú. Fueron recolectadas un total de 72 muestras de heces, 35 de la población rural (Grupo A) y 37, urbana (Grupo B). Se realizaron cuatro exámenes parasitológicos: Examen Directo, Técnica de Kato, Técnica de Sedimentación Espontánea en Tubo y Técnica de Baermann modificado en copa. La prevalencia global de parasitismo intestinal fue alta en ambos grupos (A: 88,58%; B: 67,57%). La prevalencia de parásitos intestinales fue: A. lumbricoides (A: 51,42%; B: 29,72%, p = 0,06), E. coli (A: 48,57%; B: 18,91%, p = 0,007), T. trichiura (A: 42,85%; B: 13,51%, p = 0,005), B. hominis (A: 40%; B: 32,42%, p = 0,5), E. nana (A: 37,14%; B: 16,66%, p = 0,04), G. lamblia (A: 25,71%; B: 13,51%, p = 0,19), H. nana (A: 0%; B: 5,4%), I. butschli (A: 2,85%; B: 0%), E. histolytica / E. dispar (A: 2,85%; B: 0%), S. stercoralis (A: 2,85%; B: 0%) y A. duodenale/N. americanus (A: 0%; B: 2,7%). Este estudio concluye que la parasitosis es más frecuente en la población rural que en la urbana, asociado a la pobreza, escaso sanea-miento ambiental y falta de servicios higiénicos. Debido a estas altas cifras de infección parasitaria, es importante realizar exámenes copropara-sitológicos adecuados con el fin de hacer un diagnóstico óptimo que permita brindar un tratamiento oportuno y eficaz <![CDATA[<I>Teniosis y cisticercosis en comerciantes de alimentos en mercados de una área de la ciudad de México</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100007&lng=es&nrm=iso&tlng=es In order to know the prevalence of cysticercosis and taeniosis among food traders at the markets of a political delegation in Mexico City, 262 traders were interviewed among 11 markets in Xochimilco. Coproparasitoscopic serial studies and western blot assay in sera were done looking for T. solium eggs and specific antibodies against cysticerci respectively. From interviewed persons, 115 delivered fecal samples and 110 provided blood samples. Prevalence of cysticerco-antibodies was 0,9% and T. solium eggs were not found. Traders used to sell any kind of food related with pig meat, 11,3% used to breed at home pigs and sacrificed them illegally to sell their meat. Otherwise as observed in other country (Peru), traders in pig meat food that work at markets in Mexico City seldom seem to be exposed to T. solium <![CDATA[<I>Effects of blood ingestion on patterns on the chorion of eggs of Lutzomyia ovallesi (Diptera: Psychodidae)</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100008&lng=es&nrm=iso&tlng=es Chorion patterns on eggs of Lutzomyia ovallesi fed on seven types of blood were studied using scanning microscopy. The results showed that the different blood sources produced no differences in the patterning. The eggs had a chorion with sinuous longitudinal bridges and a few transverse bridges that formed rectangular shapes. These results confirm that chorion patterns on the eggs of sand flies are a useful taxonomic parameter<hr/>Se estudio la escultura del corion de los huevos de Lutzomyia ovallesi alimentadas con siete tipos de sangre utilizando microscopia de barrido, los resultados no muestran diferencias estructuras del corion de los huevos de L. ovallesi alimentadas con las distintas fuentes sanguíneas estudiadas, presentando los huevos un corion con puentes longitudinales sinuosos con pocos puentes que se cruzan formando áreas rectan-gulares, estos resultados confirman el uso de la escultura del corion de los huevos de los flebotominos como un buen parámetro taxonómico <![CDATA[<I>Animal bait effect on the recovery of Boophilus microplus larvae from experimentally infested grass in Morelos, Mexico</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100009&lng=es&nrm=iso&tlng=es To quantify the influence of the animal presence on the percentage of Boophilus microplus larvae recovery from plots experimentally infested with this tick, it was carried out a trial in Jiutepec, Morelos, Mexico, during autumn 1999 and winter 1999-2000. For this purpose there were compared four sampling methods: human walking with chaps, bovine dressed walking, double walking flagging and double walking with baited flagging. The comparison was made on tree grasses: Andropogun gayanus(gamba), Cenchrus ciliaris (buffel) and Melinis minutiflora (molasses). It was observed the same recovery efficiency of B. microplus larvae in the four sampling methods studied in the two seasons. There were no statistical differences, although, there were differences (P < 0.05) among the grass species. The higher number of larvae recovered was recorded in the low third of the chaps, in the head and front legs of the bovine dressed, from the buffel grass and on the ventral region and legs in the other two grasses<hr/>Para cuantificar la influencia de la presencia animal en la recuperación de larvas de pastos infestados experimentalmente, se realizó un ensayo en otoño 1999 e invierno 1999-2000. Para ello se compararon 4 formas de muestreo y 3 tipos de pastos. No hubo diferencias en la recuperación de larvas entre las 4 formas, pero si, entre los tipos de pastos (p < 0,05) <![CDATA[<I>Comunidades ectoparasitarias branquiales de la pintadilla Cheilodactylus variegatus Valenciennes 1833 (Pisces: Cheilodactylidae)</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100010&lng=es&nrm=iso&tlng=es A research to study ectoparasite infracommunities from branchials on some community components of parasitefauna of 80 Cheilodactylus variegatus (32 females and 48 males) collected from Chorrillos Fishmarket, Lima, Perú, between May and July 1996 xas done. Fishes showed a standard length between 16.10 and 28.50 cm (mean = 21.58 ± 2.64). 604 specimens were collected with a mean abundance of 7.42 (1-36). The mean parasite species richness 1.3 (1-3) was not correlated with standard body length. Two fishes were not infected, 53 (66.25%) showed infection with 1 parasite species, and 24 (30%) and 1 (1.25%) had multiple infection, 2 and 3 parasite species, respectively. We found three ectoparasites: Paramicrocotyle nemadactylus (Prevalence = 91.25%; mean Intensity = 7.21; mean Abundance = 6.46 ± 7.30), Clavellotis dilatata (Prevalence = 36.25%; mean Intensity = 2.58; mean Abundance = 0.93 ± 2.03) and Caligus cheilodactylus (Prevalence = 2.50%; mean Intensity = 1; mean Abundance = 0.02). We did not find effect of sex and standard length with prevalence and mean abundance of infection of the first two ectoparasites. A slight interspecific association and covariations between abundance of. P. nemadactylus and C. dilatata employed comparatively seven indexes of association as Dice (0.470), Jaccard (0.800), Sörensen (0.888), Ochiai (0.521), Kulczynski 2 (0.578), Coeficient f (0.599), Forbe (0.906) and correlation of Pearson (r = 0.125; P = 0.271). The mean diversity in the infracommunities of C. variegatus was (H´)= 0.1722; The relative diversity was (J`) = 0.3609 and Simpson Index (C) = 0.9846. P. nemadactylus and C. dilatata showed the higher prevalence and mean abundance of infection with the first gill<hr/>Se estudiaron las comunidades de ectopa-rásitos branquiales de 80 Cheilodactylus variegatus (32 hembras y 48 machos recolectados en el mercado Chorrillo, Lima, Perú entre mayo y julio de 1996. Se encontraron 604 especímenes con un promedio de 7,4 (1-36) y se identificaron 3 especies: Paramicrotyle nemadactylus (prevalencia = 36,3%; intensidad media = 7,2; Abundancia media = 6,5 + 7,3); Clavellotis dilatata (Prevalencia= 36,3%; Intensidad media= 2,6; Abundancia media = 0,9 +2,0) y Caligus cheylodactylus (Prevalencia = 2,5%; Intensidad media = 1; Abundancia media = 0,02). No se encontró asociación entre sexo y longitud del pez y la prevalencia y abundancia de las infestaciones en los dos primeros ectoparásitos. Se analizan otros parámetros para conocer el comportamiento parasitarios en el pez estudiado <![CDATA[<I>Adherencia de Trichomonas muris in vitro</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100011&lng=es&nrm=iso&tlng=es Microorganisms adherence depends on the microorganism, on the epithelial cells and on environmental factors. The objetive was to study the action of metronidazole, CO2 atmosphere and pH on the adherence of Trichomonas muris to buccal epithelial cells. The epithelial cells were obtained by hysopping the inner faces of a healthy donor's cheeks. The cells were placed with 1,5 ml of PBS (100 cells / ml). T. muris obtained from the faecal matter of adult Rockland mice, were cultured in TYI - S -33 medium supplemented with bile and antibiotics, having a replication every 48 hours. Gibbons and van Houte technique was employed with some modifications. T. muris adhered to 100 epithelial cells, were counted. The assay was carried out with 0,1 mg metronidazole and added to PBS in a CO2 atmosphere, incubating the tubes at 37º C during 60 minutes with the following pH values: 3,5,7 and 10. 10% of T. muris adhered with metronidazole and 7% with CO2 . At pH 3 they were dead, to pH 5 and 7, 25% adhered and to pH 10, adhered 13%. At pH 7, 37ºC , in CO2 atmosphere, 27% of T. muris adhered to epithelial cells<hr/>La adherencia de microorganismos a las células depende del microorganismo, de las células epiteliales y de factores ambientales. El objetivo de este trabajo fue estudiar la acción del metronidazol, atmósfera de CO2 y pH sobre la adherencia de Trichomonas muris a células epiteliales bucales. Las células epiteliales se extrajeron por un hisopado de la cara interna de las mejillas de un dador sano, colocándose en 1,5 ml de PBS (100 células/ml) T. muris, obtenida de heces de ratones Rockland adultos, se cultivó en medio TYI-S-33 suplementado con bilis y antibióticos, repicándose cada 48 h. En la adherencia se utilizó la técnica de Gibbons y van Houte, modificada, contándose las T. muris adheridas a 100 células epiteliales. Se realizó con metronidazol agregando 0,1 mg de metronidazol al PBS; en atmósfera de CO2 incubando a 37ºC, durante 60 minutos, y a valores de pH: 3, 5, 7 y 10. El 10 % de T. muris adhirieron con metronidazol y 7% con CO2. A pH 3 se lisaron; a pH 5 y 7 adhirieron el 25% y 13% a pH 10. A pH 7, 37ºC y en atmósfera de CO2, el 27% de T. muris adhirieron a las células epiteliales <![CDATA[<I>Primer registro de</I> <I>Amblyomma longirostre (Acari: Ixodidae) en Uruguay</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100012&lng=es&nrm=iso&tlng=es Amblyomma longirostre is recorded for the first time in Uruguay. Nymphs of this species were found on a resident bird, Phylloscartes ventralis (Passeriformes: Tyrannidae), from a locality in which a potential host to adults ticks, Sphiggurus spinosus (Rodentia: Erethizontidae), also inhabits. From these facts, it is concluded that A. longirostre is, probably, resident in Uruguay. Also, this is the southernmost record for this species<hr/>Se informa por prmera vez la presencia de Amblyomma longirostre en Uruguay. Se encontraron ninfas de esta especie en un ave (Phylloscartes ventralis) de una localidad en que habita un roedor potencial huésped para esta garrapata adulta, por lo que se concluye que es probable que ella sea residente en el país. Para la garrapata esta ubicación geográfica sería la más autral descripta <![CDATA[<I>Ectoparásitos de la perdiz chilena (Nothoprocta perdicaria) en la provincia de Ñuble, Chile</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100013&lng=es&nrm=iso&tlng=es Between November 1994 and April 1995 a total of 51 chilean tinamou (Nothoprocta perdicaria) were collected in Ñuble Province (Chile) and the following ectoparasites prevalences were found; Rhynchothura teres (9.2 %), Heptapsogaster sp. (90.2 %), Menacanthus nothoproctae (39.2%), Heptapsogaster testudo (19.6%), Allosathes sp. (80.4 %), Amblyomma sp. (11.8 %) and a mite from the Trombiculidae family (15.7 %). Excepting R. teres, the other ectoparasites represent their first record in the chilean tinamou<hr/>Entre Noviembre de 1994 y abril de 1995 en la provincia de Ñuble (Chile), se capturaron 51 ejemplares de perdiz chilena (Nothoprocta perdicaria), de los que se aislaron los siguientes ectoparásitos: Rhynchothura teres (92,2%), Heptapsogaster sp. (90,2%), Menacanthus nothoproctae (39,2%), Heptapsogaster testudo (19,6%), Allosathes sp. (80,4%), Amblyomma sp. (11,8%) y ácaros de la familia Trombiculidae (15,7 %). Con excepción de R. teres, los restantes ectoparásitos diagnosticados representan el primer registro en la perdiz chilena <![CDATA[<I>New host and locality records for Urotocus fusiformis (Digenea: Leucochloridiidae) in Pteroptochos tarnii, a native bird of Patagonia (Argentina)</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100014&lng=es&nrm=iso&tlng=es Urotocus fusiformis is reported parasitizing rectum of Pteroptochos tarnii (Rhinocryptidae) a native bird from Argentinian Patagonian cold temperate forest. Specimens were mounted "in toto" and morphometric data are given. Morphology and dimensions of these specimens conform to the original description. This is the first record for this host and for Argentina<hr/>La presencia de Urotacus fusiformis en el recto de Pterptochos tarnii, un pájaro de la Patagonia Argentina, se describe por primera vez en este huésped y en este país <![CDATA[EPIDEMIOLOGICAL STUDIES OF <I>DERMATOBIA HOMINIS</I> (DIPTERA: CUTEREBRIDAE) ON DAIRY CATTLE IN A COMMUNITY OF SANTA MARIA, RIO GRANDE DO SUL STATE, BRAZIL]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100015&lng=es&nrm=iso&tlng=es With the purpose of studyng the seasonal variation and the population increase of Dermatobia hominis group's in bovines, the verification of nodes distribution caused by the grubs in the host's body area as well as the relationship between the quantity of D. hominis groups and the weather information, 79 rural properties in Santa Maria city were visited. In each property 10 animals were selected that were individually inspected. Results indicated that the highest parasitism numbers occurred during the warm months (from October to February). On the other hand, during the cold months (from April to September) the infective rates were lower or even none. Concerning the body areas, the front part showed 83,3% of the nodes, while the back one presented just 16,4% of them<hr/>Com o objetivo de estudar a variação sazonal de Dermatobia hominis em bovinos, verificou-se a presença e distribuição dos nódulos em diferentes áreas do corpo dos animais nos meses do ano. Foram visitadas 79 propriedades rurais no município de Santa Maria, Rio Grande do Sul. Em cada pro-priedade foram selecionados dez animais, sendo inspecionados individualmente. Os resultados indicaram alto parasitismo durante os meses de verão (outubro a fevereiro) e menores taxas de infecção nos meses de inverno (abril a setembro). Com relação à área corporal, 83,3% da freqüência de nódulos do berne concentrou-se na região anterior e 16,4% na região posterior do animal <![CDATA[<I>Presencia de Eustrongylides tubifex (Nematoda: Dioctophymatoidea) en la Patagonia, Argentina</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100016&lng=es&nrm=iso&tlng=es Presence of an ovigerous female of Eustrongylides tubifex has been recorded from the proventriculus of Podiceps major from a Patagonian andean lake. Two new localities for Galaxias maculatus harbouring larvae of E. tubifex are given. Prevalence of infection in lake Quillén is higher than previous reports for Lake Gutiérrez<hr/>La presencia de una hembra ovígera de Eustrongylides tubifex en el proventrículo de un Podiceps major se describe por prímera vez en un ave de un lago andino de la Patagonia Argentina. En dos nuevas localidades el parásito en Galaxias maculatus. La prevalencia de infección es mayor en el lago Quillén que la reportada previamente en el lago Gutiérrez <![CDATA[<I>Occurrence of coccidiosis in canaries</I> <I>(Serinus canarius) being kept in private captivity in the state of Pernambuco, Brazil</I>]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100017&lng=es&nrm=iso&tlng=es This study have an aim to outline the coproparasitological profile of canaries (Serinus canarius) belonging to a private captivity (Canaril Freitas) situated in state of Pernambuco, Brazil. From 327 analysed passerine birds, 167 (50,5%) were parasitized by sporozoite of genre Isospora<hr/>Este estudio tiene como objetivo, caracterizar el estado coproparasitológico de los canarios (Serinus canarius) mantenidos en caitiverio en el estado de Pernambuco, Brasil. De 327 canarios examinados, 167 (50,5%) presentaron infección por Isospora <![CDATA[<I>Nuttallia brasiliensis</I> AND <I>Theileria brasiliensis</I>, SYNONYM OF <I>Babesia brasiliensis</I> (PIROPLASMIDA: BABESIIDAE) HEMOPARASITE OF MARSUPIALS]]> http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122003000100018&lng=es&nrm=iso&tlng=es Transference of the South American opossums hemoparasite Nuttallia brasiliensis to the genus Babesia are discussed and proposed due to the pre-occupation of Nuttallia genus by mollusk species, as well as the synonym of Theileria brasiliensis to Babesia brasiliensis. Simultaneously the situation of the others members of Nuttallia and Achromaticus genus is discussed, in relation with the international rules of nomenclature and its common biologic and morphologic aspects. Also a chronological summary of B. brasiliensis reports is given<hr/>É discutida a posição taxonômica do hemoparasito de marsupiais sul-americanos, descrito como Nuttallia brasiliensis, propondo-se a sua transferência ao gênero Babesia devido à préocupação do gênero Nuttallia por espécies de moluscos, propondo-se também a sinonímia de Theileria brasiliensis em relação a Babesia brasiliensis. Simultaneamente é discutida a situação de representantes de outros gêneros como Nuttallia e Achromaticus, em relação às normas internacionais de nomenclatura, e a seus aspectos morfológicos e biológicos comuns. Também é apresentado um sumário cronológico dos relatos de B. brasiliensis