Figure 3. Comparison of DEAE Dextran with Transfectam Reagent as DNA carriers for transfection. Duplicate independent transfections were carried out using pCMVL DNA (5.0 m g) in the presence of DEAE Dextran (1.0 m g) or Transfectam (12.5 m g). Total DNA was extracted and submitted to dot blot hybridisation at two different amounts (2.0 and 1.0 m g, respectively), using linearised DIG-labelled pCMVL as a probe. Control Lane at left, duplicates of pCMVL DNA (0.6 m g), and at right, duplicates of total salmon sperm DNA (1.0 m g).