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versión On-line ISSN 0718-5839
Chilean J. Agric. Res. vol.71 no.3 Chillán set. 2011
Chilean Journal of Agricultural Research 71(3) July-September
Evaluation of Perennial Forage Legumes and Herbs in Six Mediterranean Environments
Evaluación de Leguminosas y Hierbas Forrajeras Perennes en Seis Medioambientes Mediterráneos
Daniel Real1*, Guangdi D. Li2, Steve Clark3, Tony O. Albertsen4, Richard C. Hayes2, Matt D. Denton5, Mario F. D'Antuono4, and B.S. Dear2
1Future Farm Industries Cooperative Research Centre, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. *Corresponding author (firstname.lastname@example.org).
2E.H. Graham Centre for Agricultural Innovation (NSW Department of Primary Industry and
3Department of Primary Industries, Private Bag 105, Hamilton, VIC, 3300,
4Department of Agriculture and Food,
5Department of Primary Industries, RMB
There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L.) for environments with Mediterranean-like climates common in extensive areas of Southern Australia, the Mediterranean basin, and
Key words: Bituminaria, Lotus, herbage yield, legume persistence, Australian native germplasm.
Existe una escasez de leguminosas y hierbas perennes herbáceas además de alfalfa (Medicago sativa L.) tolerantes a sequía para ambientes con clima mediterráneo como los que se encuentran en el Sur de Australia, el Mediterráneo y Chile. Por lo tanto, una colección de 174 leguminosas perennes y hierbas correspondientes a 103 especies y 32 géneros fue evaluada por su adaptación a un diverso rango de climas mediterráneos en el Sur de Australia. La distribución de las precipitaciones en los sitios experimentales varían desde moderada a altamente invernales, con un promedio de precipitaciones anuales de
Palabras clave: Bituminaria, Lotus, rendimiento de forraje, persistencia de leguminosas, germoplasma nativo australiano.
There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L.) for environments with Mediterranean-like climates common in extensive areas of southern Australia, the Mediterranean basin, and Chile (Dear et al., 2003; Li et al., 2008). In their extensive review of species adapted to Australian pastures, Gramshaw et al. (1989) listed six temperate perennial legumes widely sown in southern
While lucerne is clearly a highly drought tolerant temperate perennial legume, it is also highly competitive with annual forage species (Dear and Cocks, 1997), often leading to a monoculture which, when grazed at particular periods, can lead to animal health disorders such as bloat (FitzGerald et al., 1980) or red-gut (Gumbrell, 1997).
The case for increasing the diversity of legumes in pastures was made by Oram (1993) and Cocks (2001). The main drivers include better exploitation of diverse ecological niches, buffering against pests and diseases and achieving more sustainable soil management. Alternative perennial legume options have the potential to complement and expand the feed base for grazing livestock and provide fixed soil N and weed control options in cropping rotations. In addition, new species should assist in combating soil degradation attributable to deep drainage by using more out-of-season rainfall compared with predominantly winter growing annual pastures and cropping systems and be sufficiently persistent to incrementally dry the soil profile over successive years (Sandral et al., 2006; Dear and Ewing, 2008). The need for increased species diversity and the advantages this would convey are relevant not only to Australian pastures but to pastures in similar agroecological environments worldwide (Russi et al., 2003).
As the first step in increasing the diversity of perennial forage species Cocks (2001; 2003) and Dear et al. (2003) conducted reviews to identify a priority group of species potentially adapted to Mediterranean environments of southern Australia that warranted further evaluation. The Cooperative Research Centre for Plant-based Management of Dryland Salinity (Salinity CRC) subsequently commenced a field screening program using a multi-stage process, as described by Dear and Ewing (2008). The field studies conducted at 10 sites across southern Australia evaluated 45 species from 22 genera and identified a number of perennial legume and herbaceous species whose performance justified more detailed evaluation (Li et al., 2008; Reed et al., 2008). Based on these prior studies, accessions of the more promising species and an expanded range of previously untested species identified from a desktop review of species adapted to Mediterranean and temperate environments were sourced from collaborating partners/genetic resource centres or by collecting them in their natural environments, together with their associated root nodule bacteria (RNB) (Hughes et al., 2008).
The current study tested the production and persistence of a large group of promising herbaceous perennial species and accessions compared with lucerne at a diverse range of sites across southern Australia to identify new or unexploited species that may warrant further development for use in regions with Mediterranean-like environments.
MATERIALS AND METHODS
Six row nurseries were established across southern Australia at Wagga Wagga and Binalong in New South Wales (NSW), Katanning and Newdegate in Western Australia (WA), and Byawatha and Bealiba in Victoria. All nurseries were sown in 2005, except for Bealiba which was sown in 2006. All six experimental sites were located in non-saline, non-waterlogged areas. The two NSW sites were typical of the medium rainfall crop/pasture zones of the Riverina and Slopes regions respectively of southern NSW. Soils at both sites were acidic, with the Binalong soil containing Al and Mn levels likely to inhibit lucerne growth. The two WA sites were acidic but lucerne is able to perform well and farmers successfully grow it in these soils, most likely due to low Al levels (< 6% in the top 10cm) and negligible Mn. The site in North East Victoria at Byawatha was typical of the high rainfall livestock and crop mixed farming zone. The central Victorian site, Bealiba, was on an acid granitic soil. The soil chemical characteristics at each site are presented in Table 1.
Four of the six sites have a strongly winter-dominant rainfall pattern (Table 2) with the other two sites (Binalong and Wagga Wagga) having a non-seasonal rainfall distribution although the growing season at these sites is also mostly confined to the cooler months due to very high evaporation rates over summer. The Binalong site had the highest long term average annual rainfall (
Seasonal conditions at the sites during the experimental period showed large variation in rainfall from year to year as is common in southern Australia. Some locations such as the Wagga Wagga, Byawatha and Bealiba sites also experienced prolonged periods of below average rainfall (Table 2) which severely drought stressed the germplasm with annual rainfall in some years being only 49-58% of the long term average.
Selection of genera and species
A total of 174 legume and herb entries from 103 species in 32 genera were evaluated. These entries were sourced from the Trifolium Genetic Resource Centre at the Department of Agriculture and Food, Western Australia (DAFWA), Perth, WA or the South Australian Research and Development Institute (SARDI), Adelaide, South Australia (Hughes et al., 2008) and include cultivars, selected lines and some composites of a few entries within a species (Appendix 1). Lucerne was selected as the common control species across all sites as it is the most drought hardy and widely grown of all the available perennial legumes.
Appendix 1. Perennial legumes evaluated at Wagga Wagga (1) and Binalong (2), New South Wales; Katanning (3) and Newdegate (4), Western Australia and Byawatha (5) and Bealiba (6), Victoria from 2005 to 2008. Entry was sown in winter (W) or spring (S) and inoculated with appropriate root nodule bacteria (RNB).
The risk of introducing new weeds to the Australian environment is well recognised and all entries new to Australia underwent appropriate quarantine assessments by the Australian Quarantine and Inspection Service (AQIS), the Western Australian Quarantine and Inspection Service (WAQIS), and the internal weed risk assessment scheme of the Salinity CRC (Stone et al., 2008).
A total of 102 entries were sown at Wagga Wagga and Binalong, 95 at Katanning and Newdegate, 94 at Byawatha, and 53 at Bealiba. A paucity of seed of some lines meant that not all could be sown at all sites although there were 54 entries in common at the first five sites sown. Some entries were only sown at the later sown Bealiba site as they had not passed through quarantine in time for sowing at the other sites. The entries were selected based on (a) site characteristics of their native environments (Hughes et al., 2008); (b) previous performance in other marginal environments (Real et al., 2005); (c) performance during seed increase at the SA and WA Genetic Resource Centres (Hughes et al., 2008); (d) previous field performance in WA (G. Moore, 2004, Department of Agriculture and Food Western Australia, unpublished data), and (e) relative performance in previous field studies (Li et al., 2008).
Each entry was sown into single
A row and column design was used to restrict spatial repetition of treatments and reduce the number of treatment neighbouring concurrences in rows and columns (Cullis et al., 2006). All temperate and Mediterranean entries were sown in winter while subtropical or tropical entries were sown in spring to accommodate different temperature requirements at germination. At each site, winter and spring experiments were adjacent to each other. All entries at Bealiba were sown in winter only.
Site management and measurements
The experiments were kept weed-free by spraying the buffers with a non-selective herbicide (glyphosate,
Herbage production. All entries were assessed visually with a score of 10 as the highest and 1 as the lowest herbage DM yield at each measurement at each site. At Newdegate and Katanning all plant material from each plot was harvested, bagged, oven-dried at
Plant persistence. Basal frequency was assessed as plant persistence using a 0.2'
ASReml-R (Butler et al., 2009) was used to fit a linear mixed model to each of the response variables of DM yields, visual scores, and the square root of the basal frequency. The DM yields were also transformed by log10(x+1) to improve the normality assumptions and stabilize the variance. The winter and spring experiments were analyzed separately because the nature of the entries were mostly different for the two different sowings.
Since the number of entries is very unbalanced between the sites, we used the linear mixed model approach to predict the means for each entry (BLUPS) even though the entries may not be present at all of the sites. The linear mixed model takes the form of a simple nested repeated-measures model (assuming 'equal-correlation between times') as in Cochran and Cox (1957) for multi-environment trials. The model is nested as follows:
SITES/REP/PLOTS/TIMES with ENTRIES allocated at the PLOTS at random.
We considered the entries as RANDOM so therefore the means for the ENTRIES are called BLUPS or 'adjusted means'. Since the nature of the ENTRIES was quite unbalanced across the sites, the adjusted means would be expected to be shrunken towards the mean of all of the ENTRIES across all of the SITES. The only fixed factor was TIMES since the SITE has different entries it was considered as random.
We looked at the response over time but we noticed that the seasonality comparison of Winter vs. Summer as a strong component of this time response. An aspect of the time response was to consider a comparison between the winter and summer performance since this explained most of the variability in the time responses.
The summary of the responses was displayed with a graph (a 2-dimensional plot of effects) between the comparison over time of a "Winter" vs. "Summer" BLUP or adjusted mean. The graph shows a quadrant of responses where the response in the top-right hand corner represented the better entries compared to the ones in the bottom left-hand corner where the entries went to a zero end point were poor performers.
The averages of the standard errors of the BLUPS for the entries were used to construct approximate 95% confidence interval for the mean of the BLUPS to indicate a separation of the performance of the entries relative to the mean of the entries.
For persistence data, there were only three annual time points of measurements so the TIMES factor was subjected to a simple linear trend or slope comparison across the TIMES. We examined the slopes and final basal frequency in a similar way to the above measurements but only summarised in the manuscript results without graphical display.
Despite the different seasonal and soil conditions, the various entries had a similar performance across sites with no significant G´ E interaction in herbage yields when determined by either herbage DM yield or visual DM scores. The only significant term in the model was Entry (P ≤ 0.05). There were no other significant interactions between any other terms in the model.
Herbage DM yield. A pair-wise plot of the BLUPS of the summer vs. winter BLUPs for the log transformed DM cuts is presented in Figures 1a and 1b for the winter and spring experiments. For winter sown experiments, only one cluster of entries was formed outside the 95% confidence interval central ellipse around the grand mean with the best summer and winter performance. It consists of five entries corresponding to two species, M. sativa subsp. sativa (codes 106, 107, and 108) and M. sativa subsp. falcata L. Arcang. (124 and 125). For spring sown experiments, cluster A had the best summer and winter performance. It consisted of only entry, C. australasicum (Schltdl.) J.W. Grimes (39). Cluster B had a very good summer production and an average winter production and consisted of Lotononis bainesii Baker (72). Cluster C had a very good summer production and a low winter production and consisted of 4 entries of L. bainesii (73, 74, 75, and 76).
Herbage visual DM scores. A pair-wise plot of the BLUPS for the effects of the visual DM scores for summer and winter across the six sites is presented in Figure 2a for the winter sown experiments and Figure 2b for the spring sown experiments. An ellipse representing the 95% confidence interval from the grand mean was also plotted.
For winter sown experiments, Cluster A had the best summer and winter production. It consisted of 11 entries corresponding to six species with entry code in brackets as follows: Bituminaria bituminosa var. albomarginata C.H. Stirt (35); Cullen australasicum (39 and 40); Dorycnium hirsutum (L.) Ser. (51); Kennedia prostrata R. Br. (65); M. sativa subsp. sativa (106, 107, 108, and 117) and M. sativa subsp. falcata (122 and 123). Cluster B production in summer and winter was medium. It consisted of nine entries corresponding to seven species, C. intybus L. (37); D. hirsutum (52, 53, and 54); L. pedunculatus Cav. (81); L. corniculatus L. (85); L. cytisoides L. (88); M. sativa subsp. sativa (118); M. sativa subsp. caerulea (Less. ex Ledeb.) Schmalh (120).
For spring sown experiments, Cluster A had the highest summer and winter production. It consisted of only one entry of C. australasicum (39). Cluster B had a very good summer production and close to average winter production. It consists of five entries of L. bainesii (72, 73, 74, 75, and 76).
From the analysis of both herbage DM yield and visual DM scores of the winter and spring experiments, 12 entries were identified as the most promising for either their winter, summer, or all-year round production as follows, B. bituminosa var. albomarginata (35); C. intybus (37); C. australasicum (39); D. hirsutum (51); K. prostrata (65); L. bainesii (72); L. pedunculatus (81); L. corniculatus (85); L. cytisoides (88); M. sativa subsp. sativa (107); M. sativa subsp. caerulea (120) and M. sativa subsp. falcata (123). The identified 12 entries maintained production for the entire experimental period, with the exception of C. intybus and L. corniculatus which declined in production over time.
For the winter and spring sown experiments there was a significant G ´ E interaction for basal frequency assessed shortly after the "breaking" rains in years 2, 3, and 4. Therefore, results are presented for Wagga Wagga, Binalong, Katanning, and Newdegate separately.
For the winter sown experiments, 19, 1, 28, and 27 entries had above average basal frequency at Binalong, Wagga Wagga, Katanning, and Newdegate, respectively. Overall M. sativa subsp. falcata (122) was the only entry better than average at all sites. At Binalong, Katanning, and Newdegate, the following entries had superior persistence, C. australasicum (39); D. graecum Ser. (48), D. hirsutum (50, 52, 53, and 54); D. pentaphyllum Scop. (55 and 57); L. pedunculatus (81); L. cytisoides (89); M. sativa subsp. sativa (106, 107, and 108); M. sativa subsp. caerulea (120 and 121) and M. sativa subsp. falcata (122). At Katanning and Newdegate, the following entries had high basal frequency: D. graecum (49), D. hirsutum (51); D. pentaphyllum (56); L. creticus (87); L. cytisoides (88); L. tenuis Willd. (93) and T. tumens M. Bieb (171). Lotus corniculatus (92) had high basal frequency at Binalong and Newdegate. Trifolium ambiguum M. Bieb (158) was superior at the higher rainfall Binalong site whereas Lotus corniculatus (82) and T. physodes M. Bieb. (167, 168, and 169) performed best at Katanning. Argyrolobium uniflorum Jaub. & Spach. (9 and 10); H. boutignyanum Alleiz. (63) and M. suffruticosa DC (124) had superior persistence at Newdegate.
For the spring sown experiments, 6, 8, 7, and 8 entries had better than average persistence at Wagga Wagga, Binalong, Katanning, and Newdegate, respectively. Lotononis bainesii (72 to 76) was within the better than average group at these four sites. Glycine canescens F.J. Herm. (60) was within this group at Katanning and Newdegate, G. tabacina Benth. (61) and Cullen tenax J.W. Grimes(44) at Newdegate, C. tenax (45) at Binalong, Cullen australasicum (39) at Wagga Wagga and Binalong (not sown in spring at Katanning and Newdegate), and Astragalus cicer L. (15) at Binalong and Desmanthus acuminatus Benth. (46) at Katanning.
The 3-yr evaluation at six sites in southern Australia identified a set of 10 priority perennial forage legumes (B. bituminosa var. albomarginata; C. australasicum; D. hirsutum; K. prostrata; L. bainesii; L. pedunculatus; L. cytisoides; M. sativa subsp. sativa; M. sativa subsp. Caerulea, and M. sativa subsp. falcata) with suitable adaptation, performance, and persistence.
Six of the top 10 entries identified in the current study (C. australasicum, D. hirsutum, L. cytisoides, L. pedunculatus, M. sativa subsp. sativa and M. sativa subsp. caerulea) were also ranked in the top 10 entries by Li et al. (2008), based on their forage yield in the waterlogged soil and/or acid soil environments and in environments with less soil constraints. The good performance of C. australasicum at a number of sites in the current study (cluster A in both winter and spring sown experiments, Figures 2a, 2b) and those of Li et al. (2008) supports recent findings by Hayes et al. (2009) that found accessions of this species were the most promising of the four Cullen species they evaluated, with persistence in grazed swards equivalent to lucerne. The lower palatability of C. australasicum observed in some field studies (Hayes et al., 2009) suggests it could have a niche in lower rainfall regions where set stocking and large paddock sizes restrict the ability to implement rotational grazing. Cullen australasicum performed well in both winter and spring sown experiments in the current study suggesting it establishes readily in cooler conditions despite being most active in the warmer months.
The appearance of the two perennial Medicago subspecies sativa and caerulea in the top groups (cluster A and B) is supported by a recent study by Li et al. (2010b) of a diverse range of germplasm from the M. sativa complex in similar environments to the current study. They found that subspecies sativa was superior in terms of both persistence and productivity in less moisture stressed environments but unselected accessions of subspecies caerulea demonstrated persistence equivalent to the control lucerne cultivar Sceptre in drier environments (Li et al., 2010b). Medicago sativa subsp. caerulea is found in the drier regions of the natural distribution of the M. sativa complex (Small, 2003) and hence this subspecies is the most likely to yield highly drought tolerant germplasm and should be further exploited for semi arid Mediterranean environments. A third member of the M. sativa complex, M. sativa subsp. falcata also fell within the best performing species group in the current study. This species occupies the more northern range of the M. sativa complex in its natural environment and is regarded as very cold and drought hardy and more able to tolerate acid soils (Small, 2003). However the recent study by Li et al. (2010b) at three locations in eastern Australia found the falcata subspecies to be far less productive than subspecies sativa, caerulea and varia.
Lotononis bainseii was another species in the high priority cluster B group (Figure 2b) in the spring sown experiments. Lotononis bainseii had superior basal frequency scores at four sites reflecting the ability of this stoloniferous species to cover the ground surface. The use of this subtropical species has, to date, been restricted in southern Australia by unreliable establishment from seed and very specific seed bed and temperature requirements for emergence (Blumenthal and Hilder, 1989). Poor seedling establishment has also been a major factor in reducing adoption of the species in Uruguay (D. Real, 2008 personal communication). This species requires warm temperatures for growth (>
The Lotus genus had a number of species listed within the high priority cluster B group (Figure 2a), namely L. corniculatus, L. pedunculatus and L. cytisoides. Since this study commenced, significant progress has been reported in breeding new cultivars of L. corniculatus with greatly improved flowering and seed production at lower latitudes similar to the experimental sites of the current study (D. Real, 2007 personal communication).
The identification of B. bituminosa var. albomarginata (35) in the current study as a species of high potential (Cluster A, Figure 2a) demonstrates that it is still possible to identify new prospective species that offer valuable adaptive characteristics that can be exploited. Early studies conducted in the Canary Islands (Mendez et al., 2006) and in Italy (Pagnotta et al., 2003) also showed the promising potential of this species in Mediterranean environments. It demonstrates a high level of drought and grazing tolerance in its native environments in the Mediterranean basin and the Canary Islands (Gutman et al., 2000; Muñoz et al., 2000). It is reported to be tolerant of grazing by cattle (Sternberg et al., 2006) and goats (Ventura et al., 2000). There is substantial genetic variation within the species with B. bituminosa var. albomarginata found in coastal semi arid environments (average annual rainfall 150-
The remaining two leguminous species in the top group were D. hirsutum and K. prostrata. Dorycnium hirsutum originates in the Mediterranean region and is distributed from the Canary Islands to the Balkans (Bennett, 2003). High levels of condensed tannins and low reliability of establishment are two of the current limitations of Dorycnium species identified by Bell et al. (2008). Less is known about the Australian native perennial legume K. prostrata. Recent studies have found it to be more productive under low P conditions than lucerne (Pang et al., 2010), achieving maximum growth at 12 mg P kg-1 soil compared to an optimum of 24 mg P kg-1 for lucerne. This low P requirement most likely reflects its adaptation to the low P status of soils in its native environment and a root distribution that enhances P acquisition (Denton et al., 2006). The attractiveness of this species as a forage may be limited by the quality of its herbage. Analysis of herbage quality of the related species K. prorepens found high levels of condensed tannins (10-
Cichorium intybus was the only non leguminous species to fall within the priority groups (cluster B, Figure 2a) in the current study. This species has performed well in less stressful New Zealand environments (Li and Kemp, 2005) and in a number of recent studies in southern Australia (Li et al., 2008; Reed et al., 2008; Li et al., 2010a), but it is relatively short lived in drier environments. The productivity declined progressively in the current study. Its poor performance in drier environments is not unexpected given that no cultivars of this species have been developed specifically for low rainfall environments. Further selection to exploit the large genetic diversity within this species is warranted for increased persistence in lower rainfall environments.
For forage legumes to perform to their potential under field conditions, an effective symbiosis is essential to provide adequate biologically fixed N to the plant (Howieson et al., 2000). All leguminous species evaluated were inoculated with either commercial or experimental inoculants that were the best inoculant with the information available at the time of sowing. It is acknowledged that some non commercialized species may not have highly effective RNB available as until they demonstrate significant agronomic potential it is difficult to justify the intensive research required to identify superior inoculants. The lack of well adapted RNB for non domesticated species is always a restriction when evaluating wild or non commercial species.
A second group of 15 entries (A. uniflorum, A. cicer, C. tenax, D. acuminatus, D. graecum, D. pentaphyllum, G. canescens, G. tabacina, H. boutignyanum, L. corniculatus, L. tenuis, M. suffruticosa, T. ambiguum, T. physodes, and T. tumens) was identified for their good persistence across sites or at particular sites. Although this group may lack productivity, their persistence, often under severely drought stressed growing conditions, warrants further selection to identify more productive genotypes as the majority of these have undergone little agronomic selection.
For any of these selected species to be successful on a commercial scale there are several other characteristics that need to be considered in their development. As a minimum they would be required to i) have a broad soil and climatic adaptation to help promote a large seed market; ii) have a reliable seed establishment and the necessary seedling vigour; iii) have high seed yields and can be cost effectively harvested with a final seed cost that can compete favourably with alternative existing forage options; iv) have good grazing tolerance; v) have good seasonal forage quality especially outside the annual growing season when it is most valuable to support an animal industry; vi) have no adverse effect on animal health or risks (such as bloat) and be managed effectively with grazing. Some of the more promising species identified are being studied further by the Future Farm Industries Cooperative Research Centre (previously Salinity CRC) as new forage perennial pasture options for farmers in Mediterranean climatic zones.
The evaluation of a broad range of species supported the hypothesis that there are herbaceous perennials not currently utilised in Australia that are sufficiently persistent and productive under Australian field conditions to warrant further development for use in Mediterranean-like climate agriculture. The promising species identified based on herbage production were B. bituminosa var. albomarginata, C. australasicum, D. hirsutum, K. prostrate, L. bainesii, L. pedunculatus, L. cytisoides, M. sativa subsp. sativa, M. sativa subsp. caerula, and M. sativa subsp. falcata. These species require further study to exploit their potential development as new forage perennial pasture options.
The authors wish to thank R. Snowball and S.J. Hughes for providing the seed for these experiments. We would also like to acknowledge the contributions of Justin Tidd and Craig Lihou (NSW) and David Pearce, Bron Clark, and Jamie Smith (Victoria) for their assistance in the field work. The project received financial support from the Grains Research and Development Corporation through the CRC for Plant-based management of Dryland Salinity.
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Received: 12 January 2011.
Accepted: 26 May 2011.