versión On-line ISSN 0718-560X
Lat. Am. J. Aquat. Res. vol.39 no.2 Valparaíso jul. 2011
Lat. Am. J. Aquat. Res., 39(2): 205-213, 2011
Importance of the Río de la Plata estuarine front (southwestern Atlantic Ocean) in the feeding ecology of Argentine anchovy, Engraulis anchoita (Clupeiformes, Clupeidae)
Importancia del frente estuarial del Río de la Plata (Océano Atlántico sudoccidental) en la ecología trófica de la anchoíta argentina, Engraulis anchoita (Clupeiformes, Clupeidae)
Luciano N. Padovani1,2,3, María D. Viñas1,2,3 & Marcelo Pájaro1
1Instituto Nacional de Investigación y Desarrollo Pesquero, P.O. Box 175, B7602HSA Mar del Plata, Argentina
2Consejo Nacional de Investigaciones Científicas y Técnicas, P.O. Box 175, B7602HSA, Argentina
3Universidad Nacional de Mar del Plata, P.O. Box 175, B7602HSA, Argentina
ABSTRACT. The feeding of Engraulis anchoita was studied in the coastal reproductive habitat of the northern population during the spawning period. Stomach contents of anchovy adults and plankton samples taken during a research cruise were examined. The highest stomach fullness values were found in the Rio de la Plata estuary, particularly at stations close to the surface salinity front. Copepods, particularly those < 1 mm total length, represented by Paracalanus spp. and Oithona spp., were the most abundant prey. The dominance of small copepods in the Argentine anchovy diet is noted for the first time. Ecological implications of this fact are discussed. Other abundant preys were appendicularians, cladocerans, and fish eggs. The species composition of the zooplankton samples coincided roughly with that found in the stomach contents. However, selective feeding was observed on preys > 1 mm total length. This might be explained by a low gill rakers efficiency of adult anchovies to retain small prey. In the Rio de la Plata front, both the reported large biomasses of zooplankton and the observed intense anchovy feeding revealed the ecological significance of this front, especially when compared with the contiguous "poor in food" coastal areas.
Keywords: Engraulis anchoita, feeding, stomach content, estuarine front, copepods, southwestern Atlantic, Argentina.
RESUMEN. Se estudió la alimentación de la población norteña de Engraulis anchoita en el hábitat reproductivo costero durante su periodo de desove. Se analizaron los contenidos estomacales de adultos de anchoíta y muestras de zooplancton tomados durante un crucero de investigación. Los mayores valores de repleción estomacal se encontraron en el estuario del Río de la Plata, particularmente en estaciones cercanas al frente salino de superficie. Los copépodos fueron las presas más abundantes, particularmente especímenes < 1 mm de longitud total, representados por Paracalanus spp. y Oithona spp. Este dominio de pequeños copépodos en la dieta de la anchoíta argentina se destaca por primera vez. Se discutieron las implicancias ecológicas de este hecho. Otras presas abundantes fueron: apendicularias, cladóceros y huevos de peces. La composición de especies en las muestras de zooplancton coincidió a grandes rasgos con las encontradas en los contenidos estomacales. Sin embargo, se observó selección sobre presas > 1 mm de longitud total. Esto se puede explicar por la baja eficiencia del aparato branquial de anchoítas adultas para retener pequeñas presas. En el frente del Río de la Plata, las altas biomasas de zooplancton reportadas y la intensa alimentación observada, evidencian su significancia ecológica, especialmente cuando se compara con aguas costeras adyacentes pobres en alimento.
Palabras clave: Engraulis anchoita, alimentación, contenido estomacal, frente estuarial, copépodos, Atlántico sudoccidental, Argentina.
Engraulis anchoita is the most abundant and widespread pelagic fish in the southwest Atlantic. It plays a key role in the pelagic ecosystem of the Argentine Sea, being an important component in the diet of commercial species such as hake, squid and mackerel (Angelescu, 1982). Two distinct populations are recognized south of 34°S: the northern stock, between 34° and 41°S; and the Patagonian stock between 41° and 48°S (Hansen et al., 1984). The northern stock, object of this study, performs seasonal migrations between coastal and shelf waters. In spring and early summer the schools migrate into the coastal reproductive habitat (hereafter CRH) off the Buenos Aires Province (< 50 m depth), where massive spawning takes place (Sánchez & Ciechomski, 1995). Larvae and juveniles remain in coastal waters during their growing period. After spawning, in summer and early autumn, schools disperse into outer shelf waters to feed, accumulating a large amount of energy for the next reproductive cycle (Angelescu, 1982; Hansen & Madirolas, 1996).
E. anchoita adults are opportunistic predators on meso and macrozooplankton. They exhibit, like other engraulids, two different feeding mechanisms: filtration for smaller prey, and capture or biting for larger ones (Leong & O'Connell, 1969; Angelescu, 1982).
Previous studies indicate that anchovy feeding in the CRH was negligible when compared with the intense feeding occurring in shelf waters (Angelescu & Anganuzzi, 1981; Angelescu, 1982; Schwingel & Castello, 1994; Capitanio et al., 1997; Pájaro, 2002). In the former, individuals feed almost exclusively on small zooplankters (e.g. small copepods species, ap-pendicularians and cladocerans) while in shelf waters large zooplankters constitute their food (e.g. Calanidae copepods, euphausiids and hyperiid amphipods). This feeding pattern seems to reflect the food availability found in zooplankton studies with lower biomass in the CRH than in shelf waters (Pájaro, 2002; Viñas et al., 2002; Marrari et al., 2004). However, in northern sector of the CRH high zooplankton biomasses have been reported (Viñas et al., 2002; Marrari et al., 2004). This sector is strongly influenced by the Rio de la Plata (RdP), the second most important river of South America. Its estuarine front plays a central role in the biological production, and zooplankton aggregations are a recurrent feature in this area probably because of retention and accumulation process (Madi-rolas et al., 1997; Acha et al., 2008).
The presence of zooplankton aggregations together with the fact that high densities of anchovy schools occur frequently in RdP front (Hansen & Madirolas, 1996; Martos et al., 2005) suggest that this may be a potential anchovy feeding area into the CRH. This is not surprising since other estuarine fronts in the world have been related to important anchovy spawning and feeding grounds (Palomera, 1992; Motos et al., 1996; Peebles, 2002). However, previous studies in the CRH have not been addressed to this question. Moreover, the characterization of anchovy diet (e.g. taxonomic identification and quantification of ingested prey items) was inaccurate in these studies, especially regarding small prey.
In this work, anchovy stomach contents were analysed in order to assess differences in the feeding activity between RdP front and the rest of the CRH. Characterization of anchovy diet in the CRH was carried out and prey composition in stomach contents was compared with the prey availability in the field.
MATERIALS AND METHODS
Subantarctic coastal waters of the Buenos Aires Province (< 50 m depth) are vertically homogeneous all over the year because they are mixed by winds and tides. They are poorer in nitrate and less productive than subantarctic shelf waters (> 50 m depth) which are stratified during the warmer season. Both are separated by a semi-permanent temperature front (Carreto et al., 1995). North of 38°S the coastal waters are modified by the RdP estuary (Fig. 1). This estuarine front shows a high primary productivity, driven by the nutrient input from the river and by the vertical stability of the water column (Carreto et al., 1986). It is characterized by a salt wedge structure and strong horizontal salinity gradients. More saline and dense marine waters penetrate into the estuary on the bottom, whereas more dilute and less dense river waters flows toward the sea at the surface (Guerrero et al., 1997). The estuarine dynamics and the strong pycnoclines of the salt wedge would favor the accumulation and retention of plankton (Acha et al., 2008).
Sample collection, treatment and data analysis
The material used in this study was obtained on board the R/V "Oca Balda" on a cruise performed in coastal areas of the Buenos Aires Province between 34° and 41°S (Fig. 1). The sampling period (16 October-2 November 2004) was coincident with the peak of anchovy spawning in the study area (Sánchez & Ciechomski, 1995).
Oceanographic data were obtained in 70 stations with a CTD system (Kiel Multisonde Compact System, ME Meerestechnik Elektronic, Kiel, Germany). The salinity CTD data were calibrated by salinometer measurements of discrete water samples. Surface salinity distribution was drawn to locate the RdP estuarine front.
Anchovies were collected using a Nichimo midwa-ter trawl net with a 10 mm mesh size and immediately fixed in 10% formaldehyde. Stomach contents of 240 adult fishes of both sexes (110 to 194 mm total length) randomly selected from sixteen trawl stations (n = 15 per trawl) were examined (Fig. 1). Tows were performed both during the day and night (Table 1). Each stomach was weighed with and without food content in order to determine the weight of the ingested prey, expressed as the difference between both weights. To assess the feeding activity, mean "Stomach Fullness Index" (mSFI) was calculated for each trawl as the ratio between ingested prey and whole animal weights. According to Angelescu (1982), this index indicates the stage of satiety and ranges from empty stomach (< 0.5% of anchovy total weight) to full stomach (> 6% of anchovy total weight).
Anchovies and stomachs were weighed using a digital balance to nearest 0.01 g. No correction was applied to the weight values for fixation effect on the individuals. The dilated stomachs found empty for regurgitation during trawling, were discarded.
Zooplankton samples (n = 13) were collected with a 200 meshed 61 cm diameter Bongo net, using oblique tows in the whole water column (Fig. 1). The samples were fixed in 4% formaldehyde.
In the laboratory, both zooplankton and stomach content samples were sub-sampled into aliquots in order to quantify the zooplankters. The individuals were identified to the lowest possible taxonomic level and their total length (TL) was measured. They were placed in three size classes, < 1 and 1-2 mm TL for the mesozooplankton and > 2 mm TL for the macrozoo-plankton.
Anchovy feeding selectivity in relation to food availability was calculated as the alpha selectivity index (ai) of Chesson (1978), using the formula:
where; ri = the percentage of food item i in the stomach content; ρi= the percentage of the same item in zooplankton; n = the total number of food items assessed.
Surface salinity values in the study area were ranged between 19.5 and 33.9 (Fig. 2). The lowest values corresponded to the RdP estuary. The isohaline of 30, considered the boundary of estuarine waters (Guerrero, 1998), showed a south-eastward extension indicating a river discharge in this direction whose influence reaches 37°50'S. A well-defined frontal zone was observed with highest salinity gradients south of Sam-borombón Bay. In the rest of the study area, out of the river influence, the salinity values were above 33.
Mean Stomach Fullness Index (mSFI) of anchovy in the CRH showed a well-defined pattern with the four highest values located in RdP frontal area (Fig. 2).
This highest values were observed in specimens collected from trawl stations close to the surface salinity front, (away from the 30 isohaline): station N°4 (mSFI: 2.49), N°5 (mSFI: 2.01), N°7 (mSFI: 1.34) and N°8 (mSFI: 1.33). Some individuals reached SFI values about 6, which mean a full stomach. In the remaining stations, the mSFI were smaller than 1.00 indicating empty or partially empty stomachs.
The total bulk of anchovy diet was made up of zoo-plankton (Table 1). Mesozooplankton < 1 mm TL followed by the 1-2 mm TL fraction were dominant. Copepods were the most important group reaching almost 50% of prey items (PI). Other important groups were appendicularians (22.1% PI), cladocerans (14.7% PI), represented by Evadne nordmanni and Podon spp., and fish eggs (12.6% PI), including unidentified fish eggs and anchovy eggs. To a lesser extent veliger stages of lamellibranchs, unidentified nauplii, decapod larvae and amphipods were found.
The more abundant copepods in stomachs belonged to the < 1 mm TL fraction (Paracalanus spp. + Calanoida copepodites and Oithona spp.). Copepods size class 1-2 mm TL (Ctenocalanus vanus, Clausocalanus brevipes, Centropages brachiatus + Calanoida copepodites) was less abundant and individuals > 2 mm TL (Calanoides carinatus) were fairly rare.
Zooplankton composition and food selectivity
Mesozooplankton < 1 mm TL fraction was the most abundant in study area (Table 2). Copepods (Paracalanus spp. + Calanoida copepodites and Oithona spp.) constituted the bulk of this fraction followed by cladocerans (Evadne nordmanni and Podon spp). The 1-2 mm TL size class was represented by copepods (Ctenocalanus vanus, Clausocalanus breviPes, CentroPages brachiatus + Calanoida copepodites) and to a lesser extent by appendicularians. Anchovy eggs and unidentified fish eggs were less numerous. The least abundant macrozooplankton > 2 mm was dominated by decapod larvae and the Calanoides carinatus copepod.
From the application of the feeding selectivity index to different prey size classes, high selectivity was observed for mesozooplankton 1-2 mm TL and macrozooplankton > 2 mm TL at the expense of mesozooplankton < 1 mm (Fig. 3a). Furthermore, unidentified fish eggs showed high index in the northern sector of the study area (north of 38°S) while the appendicularians were in the southern part (south of 38°S) (Fig. 3b); what happened due to the high number of specimens found in stomach from stations 4 and 12 for unidentified fish eggs and appendicularians respectively (Table 1).
High feeding activity was detected in anchovies from the proximity of RdP surface salinity front, in contrast with a lower activity recorded in the rest of the CRH. Moreover, it is worth mentioning that during the cruise high anchovy biomasses were recorded in this area (Hansen, 2004), which agrees with the cited preference of this species for salinity frontal regions (Martos et al., 2005).
Although the low number of samples does not allow to be conclusive, these results support the hypotheses that this area could be a preferred site of intense food intake by anchovy within less productive coastal waters.
The different time of the day when samples were collected might reduce the significance of this finding, taking into account that a diel feeding cycle has been described for E. anchoita (Angelescu, 1982). However, this cycle was observed mainly in shelf waters where two periods of feeding activity at sunset and sunrise were reported. On the contrary, in the CRH this pattern was weak probably due to lower food availability. Under these conditions of limited food availability an extended feeding period has been observed in E. anchoita and several other anchovy species (Angelescu, 1982; Tudela & Palomera, 1997; Pájaro, 2002).
In this study, most of items ingested by anchovies belonged to < 1 mm and 1-2 mm TL mesozooplankton fractions. According to Angelescu (1982), these prey size (< 2 mm) would correspond to a filtration feeding mechanism in argentine anchovy. The prey spectrum found is in accordance with the taxonomic groups mentioned by other authors in the CRH where the copepods were the main prey of anchovy followed by appendicularians and cladocerans (Capitanio et al., 1997; Mianzan et al., 2001; Pájaro et al., 2007).
However, the large incidence of small copepods (< 1 mm TL) in Argentine anchovy diet is highlighted in this work because they were, for the first time, accurately identified and quantified. These results agree with those frequently reported for European anchovy (Tudela & Palomera, 1997; Plounevez & Champalbert, 1999, 2000; Borme et al, 2009).
The main small copepod species found in stomach content were Paracalanus spp., Oithona spp. and copepodite stages of Calanoida. It is known that these copepods form dense aggregations associated with saline discontinuities of the estuarine front (Viñas et al, 2002; Marrari et al., 2004).
Small copepods are important links in marine food webs, acting not only as major grazers of small phytoplankton but also preying upon heterotrophic protists which are components of the microbial loop (Turner, 2004). Thus, they might play a significant role in the energy transfer to anchovy by an alternative pathway to the traditional herbivorous food web of shelf waters: spring diatom bloom-herbivorous calanoid copepods-fish.
The species composition and relative abundance observed in zooplankton samples coincided roughly with the stomach contents. However, despite of the large abundance of small copepods in anchovy diet, a clear selectivity for prey greater than 1 mm TL was observed in this work. This might be explained by a decrease in retention efficiency of small prey by the filtering apparatus of larger fishes. In fact, an increase in gill-raker spacing with anchovy size has been previously shown (Angelescu, 1981).
Intensive predation on patches of unidentified fish eggs and appendicularians was observed in two specific hauls in northern and southern sectors of the study area respectively. Fish eggs aggregations are frequently found in the RdP estuary, belonging of about twenty fish species including anchovy that spawn pelagic eggs there (Madirolas et al., 1997; Berasategui et al., 2004). On the other hand, appendicularians are known to form dense breeding patches near the surface water during the sexual maturity period (Fenaux, 1985; Capitanio et al., 1997). Anchovy predation on these occasional prey patches confirms its opportunistic feeding habits.
Some authors suggest that the planktivorous fishes spawn in zooplankton-rich locations, which is reasonable due to the high energetic cost of reproduction (Hunter & Leong, 1981; Peebles et al., 1996; Peebles, 2002). In fact, Patagonian anchovy stock spawns in a frontal area where high biomass of copepods has been detected (Ramírez et al., 1990). However, in the CRH of the northern stock it has been widely mentioned that feeding conditions are unfavourable for anchovy adults (Angelescu, 1982; Ciechomski & Sánchez, 1983; Pájaro, 2002; Pájaro et al., 2007). Pájaro (2002) proposed that favourable conditions for the adults might not be the same for eggs and larvae, due to the fact that greatest amounts of large zooplankton also mean higher amounts of potential predators. According to this, the strategy of the fishes could consist in feeding in shelf waters, which is dominated by high concentrations of large zooplankton, and subsequently migrate into the CRH where dominant small zooplankton is suitable for feeding larvae (Pájaro, 2002).
The present findings, although based on few samples, would suggest that in RdP front, the above mentioned strategy should be revised since intense feeding occurs in this sector of the CRH. The coincidence of both feeding and spawning grounds could have important implications. It might be expected an increased reproductive potential of E. anchoita in the estuarine front as it was reported for other anchovy species spawning in food rich areas (Peebles et al., 1996). Further studies are required to confirm this observation.
The authors are indebted to Dr. Jorge Hansen, Director of the Anchovy Project of INIDEP, and the Oceanography Laboratory of INIDEP for providing the environmental data. This study was partially funded by CONICET-PIP5009; PICT 15227-03 and UNMdP 15/E269.
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Received: 15 June 2010; Accepted: 13 April 2011
Corresponding author: Luciano Padovani (email@example.com)