versión On-line ISSN 0717-9502
Int. J. Morphol. v.26 n.4 Temuco dic. 2008
Int. J. Morphol., 26(4):1029-1033, 2008.
Immunological Distribution of the Placental Lactogene and IGF-1 Receptor on Free-chorionic Villi of the Newborns Small for Gestational Age Placentae
Distribución Inmunológica del Lactógeno Placentario y los Receptores IGF-1 en Vellosidades Coriónicas Libres de los Recién Nacidos Pequeños para la Edad Gestacional
*Ruth Prieto; **Fernando Matamala & ***Mariana Rojas
* Pediatric Department, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
** Anatomy Department, Faculty of Medicine, Universidad de La Frontera, temuco, Chile.
*** Anatomy and Developmental Biology Program, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
SUMMARY: The aim of this study was observe differences in the immunological distribution of the placental lactogene and IGF-1 receptor on free-chorionic villi, between studied groups and to relate the neonatal diagnosis of PEG with morphometric and immunohystochemical characteristics of the placenta. A total of, twelve placentas from AEG newborn and twelve from PEG newborn were obtained from the Maternity Ward of Temuco, Chile. H&E, Alcian blue and Masson's trichromic stains, as well as Hematoxilyn-PAS. In the immunoperoxidase technique, were used: 1) placental lactogen (polyclonal, dilution 1:200, NCL-PLP, Novocastra) 2) Insuline-1 like growth factor (monoclonal, dilution 1:200, NCL-GHR, No vocastra). Differences between PEG and AEG placentae in the immnunostaing for placental lactogen and IGF-1 receptor in the sincitial throphoblast were not observed.
KEY WORDS: IGF-1 receptor; Free-chorionic villi; Newborns; Gestational age.
RESUMEN: El objetivo de este estudio fue observar diferencias en la distribución del lactogeno placentario y receptor del factor de crecimiento similar a la insulina, entre placentas de recién nacidos normales para la edad gestacional AEG y pequeños para la edad gestacional PEG. Un total de 12 placentas de recién nacidos AEG y 12 PEG obtenidas de la maternidad del Hospital de Temuco, Chile fueron procesadas con técnicas histológicas H&E, azul de Alcián y un método de tinción tricrómico. La técnica de inmunoperoxidasa utilizada fue: 1) lactogeno placentario (pohclonal, dilución 1:200, NCL-PLP, Novocastra) 2) Factor de crecimiento similar a la insulina (monoclonal, dilución 1:200, NCL-GHR, Novocastra). No se observaron diferencias en la distribución de lactogeno placentario ni factor de crecimiento similar a la insulina entre las placentas provenientes de recién nacidos pequeños para la edad gestacional y adecuados para la edad gestacional.
PALABRAS CLAVE: Receptor de IGF-1; Vellosidades coriónicas libres; Recién nacidos; Edad gestacional.
The placental chorionic villi elabórate hormones such as the growth hormone and placental lactogen, which determine the normal development of various fetal organs. In cases of pathological placentae, those factors would not normally be produced, generating some defects in the newborn, such as the low weight at birth (Belizan et al, 1999). The production of placental lactogen is related to the functional trophoblast, in other words, to the placental weight. The Insulin 1-Like Growth Factor (IGF-1), on the other hand, promotes the fetal growth during development before and after birth, at a time when the concentration of IGF-1 both in blood and the umbilical cord are correlated with the fetal size (Sadler, 2004; Carlson, 1999).
It has been reported that mothers of IUGR newborns have also presented, in general, a lower level of education (Frisbie et al, 1997), less prenatal care (Visscher et al, 2003), a lower weight increment during pregnancy (Newburn-Cook et al, 2002; Strauss & Dietz, 1998), gestationally-induced arterial hypertension (Doctor et al, 2001) development of cardiovascular pathologies (Eriksson et al, 2001; Oren, 2003), arterial hypertension (Do Carmo Pfnho Franco et al, 2003), inadequate lung functioning (Dezateux et al, 2004) and diabetes (Leger et al, 1997). Some ethnical differences have also been reported to exist (Alexander et al, 1999). The habit of smoking during pregnancy would also affect the placental function and henee the fetal growth (Vogt et al, 2004).
Fetal tissues expressed IGF-1 and its levéis in serum are correlated with fetal growth. Mutations in the IGF-1 gene result in manifestation of IUGR, which extend after the birth (Carlson). It also plays a role in the nutrients división in the fetal-placental unit (Gluckman & Harding, 1997). The factor IGF-1 is detected in fetal tissues from the ninth gestational week on. The importance of the IGF-1 on fetal growth is deduced from the association observed between low hormone concentrations and fetal risk seen in 57% of newborn with IUGR and a 13% perinatal mortality seem to be related to a déficit in placental lactogen secretion (Handwerger & Freemark, 2000).
The objective was to recognize the differences in the immunological distribution of the placental lactogene and IGF-1 on free-chorionic villi, between SGA and AGA (Adequate for Gestational Age) placentae. And finally, to relate the neonatal diagnosis of SGA with morphometric and immunohystochemical characteristics of the placenta, by a score or placental factor.
MATERIAL AND METHOD
A total of 24 terminal placentae, from a single gestation, with no malformations, from cesarean operation and normal labour were used. Twelve from AGA newborn (control group) and twelve from SGA newborn (study group) were obtained from the Maternity Ward, Temuco, Chile. Placentae were rinsed with tap water and fixed in buffered 10% formalin. Histological and histochemical techniques such as H&E, Alcian blue and Masson's trichromic stains, as well as Hematoxilyn-PAS (peryodic acid Schiff, McManus, 1948) and Diastase-PAS. In the immunoperoxidase technique, the following primary antibodies were used: 1) placental lactogen (polyclonal, dilution 1:200, NCL-PLP, Novocastra) to label cytoplasmic granules from the syncytial trophoblast; 2) Insuline-1 like growth factor (monoclonal, dilution 1:200, NCL-GHR, Novocastra).
For the positive control AGA newborn placentae cuts were used. And for the negative control, cuts adjacent to the ones treated with the primary antibody were used and were treated with the same technique, except that the primary antibody was replaced with non-immune mouse (Dako) serum. The following parameters were evaluated in transversal sections from the free-chorionic villi: cross section área, number of blood vessels and blood vessel área. Histological sections from placentae were analyzed at 1000X magnification, under a NIKON Eclipse E 400 light microscope equipped with a digital camera NIKON E 4500. The software Image Tool versión 3.0 was used to perform this morphometric study. The statistical software GraphPad Prisma and the Student t test were used. Results are presented as mean + SEM (xx + SEM) and p< 0.05 was considered to be statistically significant.
In the SGA group, the immunostaining for the IGF-1 receptor was evidenced in some intermedíate and stem chorionic villi, in both groups. Positive immunostaining was not observed in terminal chorionic villi. The labeling was detected in the apical región of the cytoplasm of the syncytial trophoblast as well as in the cytoplasm of 5 to 6 stromal cells per villi (Fig. 2b). Placental lactogen immunostaining was positive in the stem, intermedíate and terminal chorionic villi. This was observed as granules in the cytoplasm of the syncytial trophoblast (Fig. 2c). Both the intensity of the stain and the thickness of the granules increase in the syncytial nodes and decrease and became negative in the syncytial vascular layer or alpha zone. Negative staining was observed in the cells from the cytotrophoblast, the nuclei from the syncytial trophoblast and the stroma of the villi. Morphology of the free chorionic villi from AGA newborns. Figures la show that a denser stroma is evidenced, the basal membranes are thinner than those from the SGA group (Fig. 2a). Immunostaining for the IGF-1 receptor is similar to that shown in the SGA group (Fig. Ib) and the placental lactogen is similar to what is observed in AGA placentae (Fig. 1e).
As a result from the analysis, a score or placental factor (Table I), was obtained in order to contribute to the assessment of the placental functioning, to be used in conjunction with clinical evaluation of the new born and the modern methods of perinatal monitoring.
The inmunostaining between both groups was not significantly different, indicating that this would not be the only mechanism related to the placental weight. It must be kept in mind that the production of IGF-1 decreases with maternal undernourishment, excess of glucocorticoids andhepatic insufficieney (Sadler). The placental lactogen seems to intervene directly in the maternal metabolic adaptation to the gestation. In women, the hPL (human Placental Lactogen) acts in a coordinated manner with the somatotrope hormone, stimulating the production of maternal IGF-1 factor and modulates the intermedíate metabolism, which favors the increase of glucose available for its transfer to the fetus (Muñoz & López-Oliva, 2004). Other researchers (Torry et al, 2004), associated the IUGR, the SGA, the spontaneous miscarriages and the preeclampsia with changes in the vascular development of the placenta. When inrmunohistochemical observations of placentae from the third term were compared with severe IUGR and normal, a significant decrease in both the villi capillary number per stromal unit and in the capillary área related to the placental stroma was observed. In relation to the lack of formation of blood vessels in IUGR-SGA (Lash et al, 2001), the mechanisms involved in the formation of blood vessels are not yet known but it has been suggested that this could be a defect associated with the production of the more potent angiogenic growth factor VEGF (IGF-1 or 2). However, the results obtained through our analysis showed some differences with this study, such as an increase in the área of the terminal free-villi with no significant decrease in the number of blood capillaries, compared to the control group.
This difference can be explained by the fact that the fetal capillaries in the placenta are not static structures and can adapt to a variety of changes and stress, and also because the peripheral capillaries in the terminal villi are not represented by a highly branched network but instead are composed of elongated capillaries which bend moderately over themselves.
The intention of the score or placental factor proposed in this study is to contribute to the assessment of the placental function, strongly related with neonatal clinical care and the modern methods of perinatal evaluation. In order to use this score, it is required that the placenta be kept after the labour until the conclusión can be reached that there is no relation between neonatal pathology and the placental function. A microscopic study of the placenta should be carried out, backed up by a study protocol and follow-up procedures in each particular case.
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Correspondence to: Prof. Ruth Prieto
Facultad de Medicina Universidad de La Frontera Casilla 54-D Temuco - CHILE. Email: email@example.com
Received: 29-03-2008, Accepted: 12-10-2008.