International Journal of Morphology
versión On-line ISSN 0717-9502
Int. J. Morphol. v.26 n.4 Temuco dic. 2008
Int. J. Morphol., 26(4):941-944, 2008.
Sex Determination Using Mastoid Process Measurements in Brazilian Skulls
Determinación del Sexo Usando Mediciones en el Proceso Mastoides en Cráneos Brasileños
*,**Iván Claudio Suazo Galdames; **Daniela Alejandra Zavando Matamala; &**Ricardo Luiz Smith
* Departamento de Anatomía Normal, Universidad de Talca, Chile.
** Departamento de Morfología y Genética. Universidad Federal de São Paulo, Brasil.
SUMMARY: The mastoid process characteristics are of great utility in the diagnosis of sex. De Paiva & Segre (2003) described that sex diagnosis was possible based on the determination of the área of the triangle formed by the points porion, mastoidale, and asterion. The purpose of this study was to determine the existence of sexual dimorphism in the dimensions and the área of the mastoid triangle using statistical and discriminant function analysis. A total of 81 skulls of Brazilian individuáis that were part of the Museum of the Federal University of Sao Paulo (UNIFESP) collection were used, with sex and ages known: 50 men and 31 women between 40 and 70 years of age (mean 51.58 years, SD 7.319). Most of the lineal dimensions and the calculated áreas were higher in men than in women. Only the distance porion-mastoidale, the área of the right mastoid triangle and the total área, was higher and more significant in men with p < 0.01. The analysis of the discriminant function showed that the group of analyzed lineal dimensions presents a low discriminant capacity (Lambda of Wilks = 0.960, Canonical Correlation = 0.199); only porion-mastoidale allowed one to distinguish men's groups from women with a general accuracy of 64.2%, but with a high sensibility to classify men (93%) and a very low sensibility for women (17.7%). These results indicate that the determination of sex based on the dimensions of the mastoid triangle leads to underestimate the women, henee being of less utility in practice.
KEY WORDS: Sexual dimorphism; Sex determination; Mastoid process; Forensic anthropology.
RESUMEN: Las características del proceso mastoides son de gran utilidad para el diagnóstico del sexo. De Paiva & Segre (2003) describieron que era posible el diagnóstico del sexo, en base a la determinación del área del triángulo formado entre los puntos porion, mastoidale y asterion. El propósito de este estudio fue determinar la existencia de dimorfismo sexual en las dimensiones y el área del triángulo mastoideo, mediante análisis estadístico y de función discriminante. Se utilizaron 81 cráneos de individuos Brasileños, pertenecientes a la colección de la UNIFESP, de sexo y edad conocidos, 50 hombres y 31 mujeres, de entre 40 y 70 años (media 51.58 años, DS 7.319). La mayoría de las dimensiones lineales y las áreas calculadas fueron mayores en hombres que en mujeres. Sólo la distancia porion-mastoidale (Po-Ma), el área del triángulo mastoideo derecho y el área total resultaron mayores y significativas en hombres con p<0.01. El análisis de la función discriminante mostró que el conjunto de dimensiones lineales analizadas presenta un bajo poder discriminante (Lambda de Wilks= 0.960, Correlación Canónica= 0.199), sólo Po-Ma permitió clasificar en grupos de hombres y de mujeres con una exactitud general del 64.2%, con una alta sensibilidad para clasificar hombres (93%) y una muy baja sensibilidad para mujeres (17.7%). Estos resultados indican que la determinación del sexo en base a las dimensiones del triángulo mastoideo, tiende a subestimar a las mujeres, siendo de baja utilidad en la práctica.
PALABRAS CLAVE: Dimorfismo sexual; Determinación del sexo; Proceso mastoides; Antropología Forense.
In the skull, the temporal bone is highly resistant to physical damage; thus it is commonly found as remainder in skeletons that are very oíd; of this, the petrous portion has been described as important for sex determination (Kalmey & Rathbun, 1996).
Paiva & Segre (2003) introduced a easy technique for sex determination starting from the temporal bone, with a small observational error and with a high predictability degree. The technique is based on the triangular área calculation obtained between the points porion, mastoidale, and asterion, measured from xerographic copy of skulls. They found significant differences in the área between the right and left mastoid triangle when comparing male and female skulls, but owing to the asymmetries present in the skulls, it is recommended to observe the valué of the total área (adding right and left sides), which was also significant, so that when it is higher than or equal to 1447.40 mm2, the skull is diagnosed as male skull, and a valué near to 1260.36 mm2 or less is indicative of female skull (De Paiva & Segre).
The presence of sexual dimorphism in the mastoid triangle has been recently questioned by some authors (Kemkes & Gobel, 2006), on the basis of the studies of the asterion point localization, which position varíes in the course of life (Day & Tschabitscher, 1998); they analyzed the validity in a sample of 197 European mature skulls, indicating that the determination of the mastoid triangle área was a complementary indicator of low valué predictive for the sex diagnosis. By means of discriminant function analysis, they described that the indicator was superior for that obtained in skulls from the Institute of Anthropology of Coimbra, Portugal (72% success for female skulls and 60% for male skulls) than those from the Institute of Forensic Medicine, Mainz, Germany (61% success for male skulls and 52% for female skulls).
Considering that the original study of De Paiva & Segre was carried out on a sample of 60 skulls obtained from the Cemetery Necrópolis of Guaramos, Sao Paulo, it is possible to fhink that the indicator can have an application in the sex determination of the Brazilian population.
This situation has already been described for other morphologic indicators obtained from South African indigenous skulls, in which it was not possible to determine the presence of sexual dimorphism starting from the classic indicators described by Krogman & Iscan (1986). Other distinctive features were described to evidencíate sexual dimorñsm in indigenous South African skulls, some of which were not described for the Caucasian population (Franklin et al, 2005).
With these antecedents, the purpose of this study is to determine the existence of sexual dimorphism in the dimensions and the área of the mastoid triangle, measured directly in the skull, by means of statistical and discriminant function analysis.
MATERIALS AND METHOD
A total of 81 human skulls of Brazilian individuáis from the collection of the Museum of the Federal University of Sao Paulo (UNIFESP) were used. All the skulls had a complete registration of sex, age, origin, and cause of death. The skulls that presented trauma evidence or deformations were excluded from this study. The demographic characteristics of the analyzed sample are shown in Table I.
A quantitative blind study of the dimensions of the sides of the mastoid triangle (Fig. 1) was carried out, using the following points:
Porion (Po): superior point of the external acoustic meatus pore. Mastoidale (Ma): more inferior point of the mastoid process. Asterion (As): the meeting point of the lambdoid, occipitomastoid, and parietomastoid sutures.
The points were located and marked by a single investigator on both sides of the skull. Similar to Kemkes & Gobel, lineal measures were directly carried out using a digital caliper (0.01 mm). The mastoid triangle área was calculated by means of the Herón formula. According to the method described by De Paiva and Segre, the total área was calculated by adding the área obtained on each side.
In the case that one of the points of the skull was damaged, it was excluded from the triangle sides mensuration eliminating this área from the sample.
Using the program SPSS 15.0, the descriptive statistics of the lineal dimensions and the mastoid triangle área was calculated; the signiñcance of the mean differences in relation to sex was calculated using t-test (p < 0.05).
With the purpose to determine if those lineal dimensions allow one to differenciate males and females, the Fischer's lineal discriminant functions were determined.
In the 81 analyzed skulls, most of the lineal dimensions and the calculated áreas were higher in males than in females, but in four skulls it was not possible to determine the porion point (Po). Higher valúes were obtained in females only in Po-Ma, Po-As, and the área of the left mastoid triangle. When contrasting the equality hypothesis among the means, only Po-Ma, the área of the right mastoid triangle and the total área, was higher and more significant in males (p < 0.01). The data of the analyzed mensurations are given in Table II.
When carrying out the discriminant function analysis, we found that the group of lineal dimensions analyzed presents a low discriminant capacity (Lambda of Wilks = 0.960, Canonical Correlation = 0.199); only Po-Ma is a variable that allowed one to classify men's groups from women with a general accuracy of 64.2%, but with a high sensibility to classify males correctly (93%) and a very low sensibility for females (17.7%).
The analysis of the mastoid process characteristics is important in the determination of sex for forensic purposes. Many authors agree fhat qualitative aspects, such as fheir size, ruggedness for muscular inserts, or mastoid process inclination, are very good indicators of sexual dimorphism; however, from the quantitative point of view, their utility is discussed, because, on fhe one hand, fhere does not exist consent about fhe parameters to determine fhe height, widfh, or thickness of the mastoid process, which hinders the comparison, and on the ofher hand, the results in comparable studies have not been conclusive.
This study analyzes fhe dimensions of fhe denominated mastoid triangle, defined according to that described by De Paiva & Segre, but measured directly on the skulls. We find differences in the dimensions analyzed in male and female skulls, but fhese were less marked fhan fhose described by De Paiva & Segre in their original article. When supplementing fhe method with fhe discriminant function analysis, we find poor discriminant potential of the lineal dimensions used. It is necessary to keep in mind fhat in the original method of De Paiva and Segre, one obtains the lineal dimensions based on a plañe image by means of a xerographic copy of a structure of certain convexity (skulls), which diminishes the distance between fhe points. Measures obtained with a caliper are also linear measuring a bowstrig.
Our results also differ from those reported by Kemkes & Gobel, who obtained valúes lower fhan fhe accuracy to discrimínate sex from skulls observing fhe mastoid triangle; fhey reported a better sensibility of fhe method in females.
Based on fhese antecedents, we conclude that fhe lineal dimensions and the área of the mastoid triangle is underestimated in females, being debatable fhe use of fhose parameters for sex diagnosis in practice.
Day, J. D.; Tschabitscher, M. Anatomic position of the asterion. Neurosurgery, 42:198-9, 1998. [ Links ]
De Paiva, L. A. & Segre, M. Sexing the human skull through the mastoid process. Rev. Hosp. Clin. Fac. Med. Sao Paulo, 58:15-20,2003. [ Links ]
Franklin, D.; Freedman, L. & Milne, N. Sexual dimorphism and discriminant function sexing in indigenous South African crania. Homo, 55: 213-28, 2005. [ Links ]
Kalmey, J. K. & Rathbun, T. A. Sex determination by discriminant function analysis of the petrous portion of the temporal bone. J. Forensic Sel, 47:865-7,1996 [ Links ]
Kemkes, A. & Gobel, T. Metric assessment of the "mastoid triangle" for sex determination: a validation study. J. Forensic ScL 57:985-9, 2006. [ Links ]
Krogman, W. E. & Iscan, M.Y. The Human Skeleton in Forensic Medicine. Springfield, 1986. [ Links ]
Correspondence to: Prof. Dr. Iván Suazo Galdames
Departamento de Anatomía Normal, Universidad de Talca. Avenida Lircay s/n Oficina N°104,Talca - CHILE.
Received: 22-08-2008, Accepted: 15-09-2008.