versión On-line ISSN 0717-7356
Chungará (Arica) v.37 n.1 Arica jun. 2005
Volumen 37, N 1, 2005. Páginas 85-90
ASPECTS OF INGESTION TRANSMISSION OF CHAGAS DISEASE IDENTIFIED IN MUMMIES AND THEIR COPROLITES
TRANSMISIÓN DE LA ENFERMEDAD DE CHAGAS IDENTIFICADA EN COPROLITOS DE MOMIAS
Arthur C. Aufderheide*, Wilmar Salo*, Michael Madden*, John Streitz*, Katharina Dittmar de la Cruz**, Jane Buikstra***, Bernardo Arriaza****, and Lorentz E. Wittmers, Jr.*
* Paleobiology Laboratory, Dept. Pathology, University of Minnesota, Duluth School of Medicine, Duluth, MN 55812.
Molecular study of Trypanosoma cruzi (T. cruzi) anciant DNA (aDNA) in the soft (nonskeletal) tissues of 283 naturally (spontaneously) mummified bodies from coastal sites located in southern Peru and northern Chile demonstrated a Chagas disease prevalence rate of about 41% over the past 9,000 years. This rate is similar to that of several endemic areas within this region prior to initiation of public health control programs. This report focuses on the presence of T. cruzi aDNA in the coprolites of some of these mummies. Review of the possible mechanisms that may explain the presence of this parasite in the coprolites indicates numerous antemortem and postmortem circumstances that conceivably could have been responsible. In given conditions, all of these may need to be considered. These considerations indicate that the presence of T. cruzi aDNA in mummy coprolites cannot categorically be considered as evidence of ingestion of the parasite.
Key words: coprolites, aDNA, american trypanosomiasis.
El estudio molecular del tejido blando de 283 de cuerpos momificados en forma natural proveniente de la costa del sur de Perú y norte de Chile revela la presencia de Tripanosoma cruzi a partir de 9.000 años atrás con una prevalencia de un 41%. Este valor es parecido a áreas de estas regiones donde la enfermedad era endémica, antes que se iniciaran los programas de control de la salud. Este ensayo discute la presencia de ADNa de T. cruzi en los coprolitos de momias y revisa los posibles mecanismos de estos parásitos en los coprolitos. La presencia de T. cruzi en los coprolitos de momia no necesariamente indica que hubo una ingesta de este parásito.
Palabras claves: coprolitos, ADNa, tripanosomiasis americana.
Rothhammer et al. (1984) reported on nine mummified cases with Chagas disease (American trypanosomiasis) symptomatology from Quebrada de Tarapaca in northern Chile. Their gross pathological finding included evidence of megacolon and cardiomegaly. The mummy's intestines were abnormally packed with feces, suggesting absence of peristalsis. These inhumations dated to 470 B.C. to 350 A.D. It is interesting that our biomechemical studies of Chagas disease in the coastal area of southern Peru and northern Chile is confirming the existence of this disease (Aufderheide et al. 2004). Using polymerase chain reaction (PCR) and molecular probe methodology we tested for the presence of a segment of Trypanosoma cruzi ancient DNA (aDNA) in soft (nonskeletal) tissues dissected from 283 spontaneously mummified human remains dating to the period between 7,000 B.C. and A.D. 1,800 from that region. Results indicated that 41% of those populations had been infected with T. cruzi.
Not included in that report were the results of similar tests on coprolites from 27 of these mummies. This communication deals with the results of analysis of those coprolites and discusses their significance. In addition, head lice from heavily-infested hair of mummies of the Chiribaya cultural group (Tables 1 and 2) were also analyzed.
Material and Methods
Details of the mummies and the biochemical procedures employed have been reported previously (Aufderheide et al. 2004). Briefly, the mummy soft tissue specimens were acquired by dissection principally with permission of the Dept. of Anthropology of the University of Tarapaca in Arica, Chile. The lice were studied by permission of Jane Buikstra, Ph.D., director of the archaeological excavation of the Chiribaya burial sites in the Osmore Valley of southern Peru near the seaport of Ilo in 1990 as part of Project Cuntisuyu. Ancient DNA was extracted, PCR amplified and characterized by hybridization. Specimens of lice were collected as reported in Dittmar de la Cruz (2003). The collected lice were retained in a refrigerated solution of 100 % ethanol until analyzed. A single louse was removed from the ethanol and allowed to dry 20 minutes. The louse was transferred to the PCR tube using sterile forceps. The louse was then crushed with a flame-sealed Pasteur pipette and the PCR solution was added. Subsequent processing was the same as for tissue specimens except that the amplicon was characterized by electrophoresis. Positive results were confirmed by sequencing the isolated band. Each of the 57 lice was studied in this way. Coprolite samples were pulverized under liquid nitrogen in a mechanical mill (Spex CertiPrep Freezer/Mill (Spex International, Metuchen, New Jersey, USA). 400 mg of the powdered sample was then selected for analysis and processed in the same manner as were the soft tissue samples (Aufderheide et al. 2004).
T. cruzi aDNA was identified in 8 of 15 coprolites found in the colon of mummies whose soft tissues also harbored this parasite, but in none of 12 coprolites from mummies whose soft tissues yielded no trypanosomal DNA. In addition, this parasite's DNA was also present in two of 57 head lice (Pediculus humanus). Tables 1, 2 and 3 detail results of the coprolite and lice analyses.
Coprolites were available from 15 mummies whose soft tissues had demonstrated the presence of T. cruzi; eight of these coprolites revealed a positive reaction (Table 1). None of the coprolites were positive in the group of 12 mummies whose soft tissues tested negative for T. cruzi (Table 2). This difference is statistically significant; chi square analysis reveals that p < .005. The following comments are directed at the possible interpretation of these findings: how could T. cruzi aDNA have come to be incorporated into these eight coprolites?
Transmission of Chagas disease to humans normally is carried out by one of the blood-sucking insect vectors of the Reduviid family, usually Triatoma infestans. Defecation of trypanosome-laden vector's feces on the human's skin follows the vector's bite and blood meal. The trypanosomes gain access to the human tissues when the itching wound is rubbed, dragging the trypanosomes from the skin surface into the wound or onto the conjunctiva. However, oral ingestion is a well documented mechanism. It has occurred in humans as a laboratory accident while pipetting a solution of T. cruzi (Brener 1984). Experimental transmission has been observed by feeding an infected vector insect (Rhodnius prolixus) to hungry opossums (Yaeger 1971) and also by feeding both infected mice carcasses and infected vectors to wood rats (Bastien 1998:194; Marsden, 1962). Acha and Szyfnes (1991) suggest that the high frequency of infected cats in households with such vectors is the result of domestic cats feeding on infected mice.
Ingestion of infected, uncooked meat
In our tested mummies, oral transmission may have occurred by ingestion of infected, uncooked meat. One source of such meat may have been guinea pigs (Cavia porcellus). Many authors have noted that the common Andean practice of raising guinea pigs for consumption in cages within human dwellings invites infestation by infected Chagas insect vectors (Herrer 1964). From 10 to 61% of such guinea pigs have been found to be infected by T. cruzi. Guinea pigs thrive at altitudes up to 4000 meters and serve as hosts, reservoir and disseminators of this infection (Herrer 1964). At least some of our studied mummies were part of a cultural group (Chiribaya, A.D. 900-1,100) whose excavated dwellings included such guinea pig cages (Dittmar de la Cruz 2003). We know that at least some of our studied mummies' diets included consumption of uncooked meat because their coprolites contained ova of the fish tapeworm Diphyllobothrium pacificum (Araujo et al. 1983). Infected vectors prowling a house at night are also known to defecate on surfaces they traverse. These surfaces could include exposed food items, such as meat exposed to the open air to dry following the butchering of a large mammal. We may conclude that consumption of infected, uncooked meat is a candidate for explanation of finding T. cruzi aDNA in our mummy coprolites.
Ingestion of infected head lice
We also considered the possibility of transmission by head lice (Pediculus humanus). The usual Chagas disease vector in domiciliary settings such as Triatoma infestans, tolerates completion of the T. cruzi life cycle within its intestines after it ingests an infected blood meal. The parasite flourishes in the vector insect's rectum ("posterior station"). When it ingests a blood meal, the insect expels its rectal contents (teeming with T. cruzi) on the victim's skin surface. The T. cruzi are carried into the wound or nearby conjunctiva when the victim rubs the itching wound area. T. cruzi cannot survive the head louse's intestinal environment (Khachoian and Arakelian 1978). However, if the head louse were ingested by a human shortly after its bite, the T. cruzi released in the ingesting human's intestine could infect that individual. Precedence for such a sequence of events has been reported by Argañaraz et al. (2001) where infected lice, ingested during observed "grooming" (removal of lice by a primate from another primate and then promptly eats the lice), apparently spread the infection throughout a baboon primate research colony. Some of our mummy populations, especially the Chiribaya (listed in Tables 1 and 2) had a heavy affliction of head lice. Testing of 57 individual lice collected from the mummy hair of that cultural group demonstrated T. cruzi aDNA in two of these that had a positive reaction (Table 3).
While the lice infestation was obvious in some of our tested mummy populations, we have no direct evidence that such individuals indulged in grooming. We do know that some Native North Americans did indulge in grooming practices. Of these, the most well-documented is by Samuel Hearne (1958:128-129) during his famous trek from the Hudson Bay post to the Coppermine River and back. Thus, while the evidence is weak, we cannot exclude ingestion of infected head lice as an explanation for finding T. cruzi aDNA in mummy coprolites.
A third possible source of T. cruzi ingestion (indirectly) is the conceivable lodging of peripheral blood flagellated T. cruzi parasites in pulmonary capillaries. Their size is such that this might occur occasionally. Some of these could escape from the capillary of the lung's interalveolar septum, migrate into the intra-alveolar space, enter the terminal bronchiole and be swept upward on the cilia-propelled mucous blanket lining the bronchi, being ultimately discharged into the pharynx and then swallowed, entering the intestinal tract. While this is conceivable, it has no supportive or experimental evidence.
Experimental evidence in acutely infected chagasic mice has demonstrated histological lesions of acute, necrotizing arteriolitis in the smooth muscle of the intestinal wall (Okumura et al. 1960). Extension to the gut mucosa of such necrotic areas could provide T. cruzi access to the intestinal content. Our test results (Table 1) indicate that T. cruzi was found only in coprolites of mummies whose soft tissues were also positive. Certainly parasitemic invasion of the intestinal wall remains a candidate for explaining our results.
T. cruzi DNA incorporation into the host genome
Investigations by Teixeira et al. (1994) and Nitz et al. (2004) have indicated that T. cruzi kinetoplast and also nuclear DNA can be integrated into the host genome. The cells of the human gut mucosa turn over rapidly, with complete replacement every 4 to 6 days (Kumar et al. 2005:829). If such DNA integration occurred in our tested mummies, T. cruzi DNA may have been carried into the tested coprolites by exfoliated cells of the intestinal mucosa. Until the host genome of our mummies is investigated for such possible integration, this mechanism remains a possible explanation of our results.
Postmortem migration of T. cruzi parasites
Most of our tested mummies became desiccated via spontaneous mummification. Under these conditions the intestinal mucosa degenerates rapidly immediately after death prior to arrest of decay by desiccation in many of the other body tissues. This reduces the gut wall to a thin, translucent membrane. The mucosa with its blood vessels that may contain parasitemic T. cruzi parasites liquifies after death and can be absorbed into the coprolite in the gut lumen. This is appears to be the most probable of the possibilities discussed above, a conclusion supported by the statistically significant observation that T. cruzi aDNA was present in coprolites only in those mummies in whom it was also present in the mummies' soft tissues.
T. cruzi aDNA was found in coprolites of spontaneously mummified mummies' intestines. A review of the possible routes of T. cruzi transport, reaching intestinal coprolites includes ingestion, parasitemic dissemination, host genome incorporation of T. cruzi aDNA and postmortem migration. While any of these are conceivable, the postmortem route appears to be the most probable. It is clear that the presence of T. cruzi aDNA in the coprolites of mummies cannot always be considered evidence of T. cruzi ingestion during life.
Acha, P.N. and B. Szyfres 1991 Zoonoses and Communicable Disease Common to Man and Animals. Pan American Health Organization. Washington, D.C. [ Links ]
Araujo, A., F. Ferreira, U.E. Confalonieri and L. Nuñez 1983 Eggs of Diphyllobothrium pacificum in preColumbian human coprolites. Paleopathology Newsletter 41:11-13. [ Links ]
Argañaraz, R., G.B. Hubbard, L.A. Ramos, A.L. Ford, N. Nitz, M.M. Leland, J.L. Vandenberg and A.R.L. Teixeira 2001 Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons. Revista do Instituto de Medicina Tropical, São Paulo 6:271-276. [ Links ]
Aufderheide, A.C., W. Salo, M. Madden, J. Streitz, J. Buikstra, F. Guhl, B. Arriaza, C. Renier, L.E. Wittmers, Jr., G. Fornaciari and M. Allison. 2004 A 9000-year record of Chagas disease. Proceedings of the National Academy of Sciences 101:2034-2039. [ Links ]
Bastien, J.W. 1998 The Kiss of Death. University of Utah Press, Salt Lake City. [ Links ]
Brener, Z. 1984 Laboratory-acquired Chagas' disease: an endemic disease among parasitologists? In Genes and Antigens of Parasites, edited by C. M. Morel, pp. 3-9. Fundação Oswald Cruz, Rio de Janeiro. [ Links ]
Dittmar de la Cruz, K., R. Ribbeck and A. Daugschies 2003 Paläoparasitologische Analyse von Meerschweinchen-mumien der Chiribaya-Kultur (900-100 AD), Moquegue-Tal, Peru (Paleoparasitological analysis of guinea pigs of the Chiribaya culture (900-100 AD), Moquegua Valley, Peru). Berlin und München Tierarztliche Wochenschrift 116:45-49. [ Links ]
Hearne, S. 1958 Journey to the Northern Ocean. Edited by R. Glover, Macmillan, New York. [ Links ]
Herrer, A. 1964 The epidemiological importance of the guinea pig. Tropical and Geographic Medicine 2:146-151. [ Links ]
Khachoian, V.I. and L.A. Arakelian 1978 Case of transmission of the rat trypanosome by lice (in Russian). Parazitologiia 12:451-453. [ Links ]
Kumar, V., A.K. Abbas and N. Fausto, editors. 2005 Pathologic Basis of Disease. Elsevier, Philadelphia. [ Links ]
Marsden, P.D. 1967 Trypanosoma cruzi infections in CFI mice. Annals of Tropical Medicine and Parasitology 61:62-67. [ Links ]
Nitz, N., C. Gomes, A. Rosa, M.R. D' Souza-Ault, F. Moreno, L. Lauria-Pires, R.J. Nascimento and A.R.L. Teixeira 2004 Heritable integration of kDNA minicircle sequences from Trypanosoma cruzi into the avian genome: Insights into human Chagas disease. Cell 18:175-186. [ Links ]
Okumura, M, T. de Brito, L.H. Pereira da Silva, A. Carvalho da Silva and A. Correa Netto 1960 The pathology of experimental Chagas disease in mice: digestive tract changes, with a reference to necrotizing arteritis. Revista do Instituto de Medicina Tropical, São Paulo 2:17-28. [ Links ]
Rothhammer, F., V. Standen, L. Núñez, M. Allison y B. Arriaza 1984 Origen y desarrollo de la Tripanosomiasis en el área centro sur andina. Chungara 12:155-160. [ Links ]
Teixeira, A.R., E.R. Aragañaraz, L.H. Freitas, Jr., Z.G.M. Laçava, J.M. Santana and H. Luna 1994 Possible integration of Trypanosoma cruzi kDNA minicircles into the host genome by infection. Mutational Research 305:197-209. [ Links ]
Yaeger, R.G. 1971 Transmission of Trypanosoma cruzi infection to opossums via the oral route. Journal of Parasitology 57:1365-1376. [ Links ]
Recibido: febrero 2005. Aceptado: marzo 2005.