SciELO - Scientific Electronic Library Online

 
vol.49 número1Clinical presentation and biochemical profile of horses during induction and treatment of hypocalcemiaRuminants are not a reservoir of enteroaggregative Escherichia coli índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Austral journal of veterinary sciences

versão impressa ISSN 0719-8000versão On-line ISSN 0719-8132

Resumo

CARMONA, Jorge U.; RIOS, Diana L.  e  LOPEZ, Catalina. Effects of two platelet-rich gel supernatants at two concentrations on healthy cartilage explants from horses. Austral j. vet. sci. [online]. 2017, vol.49, n.1, pp.15-23. ISSN 0719-8000.  http://dx.doi.org/10.4067/S0719-81322017000100015.

Platelet-rich plasma is used as a treatment of arthropathies in horses. However, there is lack of in vitro information about the mechanism of action of this substance and its effects on healthy cartilage of these animals. The aims were: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte- and platelet-rich gel (L-PRG) and pure platelet rich gel (P-PRG) supernatants on the production/degradation in normal cartilage explants (CEs) of platelet-derived growth factor isoform BB, transforming growth factor beta-1, tumour necrosis factor alpha, interleukin (IL) 4 (IL-4), and IL-1 receptor antagonist (IL-1ra); and (2) to study possible correlations of these molecules with their respective PRG supernatant treatments. CEs from six horses were cultured for 96 h with L-PRG and P-PRG supernatants at 25 and 50% concentrations, respectively. CE culture media were changed each 48 h and used for determination, by ELISA, of the molecules. L-PRG and P-PRG supernatants at 25 and 50% concentrations influenced the molecular anti-inflammatory profile of CE groups cultured with these substances. 50% L-PRG supernatant produced the most robust pro-inflammatory effects when compared to the CE control group and the CE group treated with the other PRG supernatant concentrations. In general, PRG supernatants induced pro-inflammatory effects in normal CEs.

Palavras-chave : cartilage; cytokines; growth factors; horse; platelet-rich plasma..

        · resumo em Espanhol     · texto em Inglês     · Inglês ( pdf )