Resumo

HERNANDEZ-RIVERA, M.A et al. OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp. J. Soil Sci. Plant Nutr. [online]. 2011, vol.11, n.1, pp. 29-43. ISSN 0718-9516.  doi: 10.4067/S0718-95162011000100003.

In México, there are extensive areas polluted by oil spills. Currently, bioremediation technologies have been developed, using microorganisms to clean up oil sites. In this study, we evaluated the development of a hydrocarbonoclastic bacterial strain, using a completely randomized 3x3x4 factor arrangement: three temperatures, three pH, and four nutrients. We collected samples of soil contaminated with 3.45 x 10⁵ mg kg^-1 (345,000 ppm) Total Petroleum Hydrocarbons (TPH), located at the Ejido José N. Rovirosa, Huimanguillo, Tabasco, México. The samples were grown in a culture medium Nutrient Agar (NA), obtaining a bacterial strain, which was characterized and classified as Proteus sp. The strain was grown in a combined carbon culture medium and then in a liquid mineral medium with crude oil as sole carbon source. Analysis of variance and mean test were performed, using the SPSS-11.0 statistical software. The microorganisms showed the highest population growth in the treatment N_2=Triple17pH_1=4.029°C with a value of 4.6 x 10¹⁰ CFU mL^-1. To reach, by bioaugmentation, the same development of Proteus sp in a conditioned soil would allow us to implement a potential bioremediation strategy for solving the problem of soils contaminated with hydrocarbons in the state of Tabasco in particular, and in Mexico in general.

Palavras-chave : hydrocarbon degrading cells; optimal growth; Proteus sp.