SciELO - Scientific Electronic Library Online

 
vol.71 número3Implantación y Acumulación de Forraje de Plantago ‘Ceres Tonic’ Puro y en Mezcla con GramíneasCapacidad de Alojamiento de Plantas Hortícolas para Plagas de Insectos en Brasil índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Chilean journal of agricultural research

versión On-line ISSN 0718-5839

Resumen

GARCIA-GONZALES, Rolando et al. In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots. Chilean J. Agric. Res. [online]. 2011, vol.71, n.3, pp. 376-382. ISSN 0718-5839.  http://dx.doi.org/10.4067/S0718-58392011000300005.

Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L-1 6-bencilaminopurine and 3 mg L-1 naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds.

Palabras clave : Organogenesis; in vitro propagation; in vitro rooting; plant growth regulators.

        · resumen en Español     · texto en Inglés     · pdf en Inglés