Latin american journal of aquatic research
On-line version ISSN 0718-560X
CATCOPARCO, Christian; SILVA, Alfonso and DUPRE, Enrique. Cryopreservation of flounder Paralichthys adspersus spermatozoa. Lat. Am. J. Aquat. Res. [online]. 2012, vol.40, n.2, pp.259-266. ISSN 0718-560X.
To optimize the techniques of captive breeding of flounder Paralichthys adspersus, a methodology was developed for the cryopreservation of spermatozoa of this species. The effect on sperm motility post-thawing, using three different concentrations (1.0; 1.5 and 2.0 M) of DMSO as cryoprotective agent and five different freezing rates -7.5; -10; -12.5; -20 and -30°C min-1, with an automatic programmable freezer was evaluated. The highest percentages of post-thawing sperm motility were obtained by freezing sperm at -10°C min-1, no significant differences (P < 0.05) were founded between the three different concentrations of DMSO used. Subsequently, we evaluated the effect of a non-permeable cryo-additive (chicken egg yolk, VHG), in order to obtain an increasing of the percentage of sperm motility. We used a cryoprotectant solution including DMSO at three different concentrations adding 10% VHG v/v. The highest percentages of sperm motility (71.71 ± 13%) were obtained at the freezing rate of -10°C min-1 without significant differences between the three concentrations of the cryoprotectant solution in which the sample was incubated sperm (P < 0.05). A high significant difference between the sperm motility percentages post-thawing using DMSO and DMSO with VHG, was observed.
Keywords : cryoaditives; crioprotectant; sperm motility; freezing rate; flounder; Chile.