Revista de otorrinolaringología y cirugía de cabeza y cuello
versión On-line ISSN 0718-4816
GONZALEZ G, Claudia et al. TNFα modifies ciliary beat frequency in response to viscous overload, associated to changes in intracellular calcium in human respiratory ciliated cells. Rev. Otorrinolaringol. Cir. Cabeza Cuello [online]. 2010, vol.70, n.2, pp. 99-108. ISSN 0718-4816. doi: 10.4067/S0718-48162010000200002.
Introduction: Pathologic sin onasal secretions and elevated levels of tumor necrosis factor alpha (TNFα) have been in oted in sin us mucosa ofpatients with chronic sinusitis. Respiratory ciliated cells have a functional reserve that allows them to autoregulate their ciliary beat in response to the changesin viscosity, modify in g intraacellular calcium levels [Ca+2]ic. Aim: Our goal was to determinate if TNFα affect this autoregulation to viscosity and calcium homeostasis. Material and Method: Primary cultures from adenoid tissue expiants. Ciliary beat frequency (CBF) was recorded usin g microphotodensitometry Cultures viere treated with TNFα (10 ng/ml) or control during 24 and 48 hours. Viscosity wasincreased by add in g dextran 500 10% and 20%. [Ca+2]ic was determined in cells loaded with Fura-2AM. Results: 48 hours treatment with TNFα produced a significant decrease in CBF at low viscosity significant increase in [Ca+2]ic and greater decrese in CBF in cultures treated with thapsigargin (endoplasmic calcium-ATPase pump blocker). Conclusions: After 48 hours ofexposure to TNFα a negative effect in the adaptation mechanism to a low viscous media is observed in ciliated cells, probably secondary to changesin homeostasis of [Ca+2]ic.
Palabras clave : Ciliary beat frequency tumor necrosis factor alpha; TNF; viscosity; intraacellular calcium.