Revista chilena de cirugía
versión On-line ISSN 0718-4026
OLIVARES R, Gabriel et al. Ligation of left anterior descending coronary artery: Surgical technique. Rev Chil Cir [online]. 2012, vol.64, n.1, pp. 15-18. ISSN 0718-4026. http://dx.doi.org/10.4067/S0718-40262012000100003.
Background: An experimental model for ligation of the left anterior descending coronary artery (ADA) in rats has been commonly used in research into ischemic cardiopathy. However, this procedure is very difficult, with a high mortality rate. There is little information in the literature regarding its technical aspects. Our objective was to describe a surgical technique for ADA ligation in rats. Material and Method: Fifty-one Sprague-Dawley male rats weighting 230 ± 20 gr were anaesthetized and connected to mechanical ventilation. Through a left-side thoracothomy we ligated the ADA 2 mm below the junction of the pulmonary artery and the left atrial appendage. The next day we performed transthoracic echocardiography to confirm our results. Results: The mortality rate was 7.8% (4 out of 51). The main cause of death was hemorrhage during surgery. The average time for the procedure was 45 ± 5 min. We confirmed hypokinesia of the anterior lateral wall in 100% of the surviving rats. Discussion: This procedure was reproducible and safe, with less than 10% mortality.
Palabras llave : Ligation; coronary vessels; rats; animal experimentation; heart surgical procedure.