Revista chilena de cirugía
versión On-line ISSN 0718-4026
OLIVARES R, GABRIEL; PARRA D, CLAUDIO y TRONCOSO C, PABLO. Heterotopic heart transplantation in rats. Rev Chil Cir [online]. 2008, vol.60, n.3, pp. 194-197. ISSN 0718-4026. doi: 10.4067/S0718-40262008000300005.
Introduction: Heterotopic heart transplantation in rats is an experimental research model, specially used to study immunosuppression. Aim: To develop a model of heterotopic heart transplantation in rats. Material and methods: Lewis rats (as receptors) and Brown Norway rats (as donors), weighing 200 to 350 g, were used. For procurement, a butterfly chest incisión was done, a metallic clip was placed in inferior and superior vena cava to infuse a physiologic solution with heparin, stopping cardiac beats and sectioning pulmonary and aortic arteries. Pulmonary and cava veins were ligated jointly and sectioned. In the receptor rats, aorta and abdominal vena cava were identified and an atraumatic bulldog vascular clamp was placed. Termino lateral anastomoses between donor ascending aorta and receptor abdominal aorta, and between donor pulmonary artery and receptor vena cava were performed with continuous microsuture. Transplantation was considered successful when the graft was functional for more than 24 hours, determined palpating beats in the abdomen. Results: Eighty rats were transplanted. We main causes of graft loss were a prolonged operative time and postoperative hemorrhage. The technique was modified, using a unique ligation of cavas and pulmonary veins, elliptic and lateral venotomy, ligation of a vessel that is parallel to aorta that avoids lethal hemorrhages and postoperative fluid replacement. Discussion: This is a reproducible model. The technical modifications introduced, reduce the lapse of ischemia and increase graft survival
Palabras clave : Heart transplantation; rats; graft survival; microsurgery.