versión On-line ISSN 0718-3429
TERUMI ITAKO, Adriana et al. Effect of chemicals on the bacterial spot (Xanthomonas perforans) and the activation of pathogenesis-related proteins in tomato. Idesia [online]. 2012, vol.30, n.2, pp. 85-92. ISSN 0718-3429. http://dx.doi.org/10.4067/S0718-34292012000200011.
The present study aims to evaluate the effect of fungicides and antibiotics to control bacterial spot (Xanthomonas perforans) in tomato, and the activation of pathogenesis-related proteins. Hybrid tomato AP 529 was used to assess the severity of disease. The treatments consisted of spraying with acibenzolar-S-methyl, fluazinam, pyraclostrobin, pyraclostrobin + methiran, copper oxychloride, copper oxychloride and mancozeb + oxytetracycline, and inoculated and non-inoculated controls. After three days of treatment, all plants were inoculated with X. perforans (106 CFU / mL). Leaf discs were collected for assessment of peroxidase, polyphenol oxidase, β-1,3 glucanase, phenylalanine ammonia lyase and protease. The area under the disease progress curve (AUDPC) was calculated with the data of severity. All treatments had reduced AUDPC compared to the inoculated control. Fungicides acibenzolar-S-methyl, pyraclostrobin, and pyraclostrobin + methiran had more satisfactory results in reducing the severity of bacterial spot on tomato. The products based on pyraclostrobin together with acibenzolar-S-methyl induced enzymatic activities of peroxidase, polyphenoloxidase and β-1,3 glucanase, indicating that these products may be related to the induction of resistance to bacterial spot on tomato plants.
Palabras clave : inducing resistance; peroxidase, β-1,3 glucanase; acibenzolar-S-methyl and pyraclostrobin.