versão On-line ISSN 0718-0764
ANSELMO, Ricardo J e BARRIOS, Hebe A. New Genetic Profiles of Salmonella Enteritidis identified in Lujan, Argentina. Inf. tecnol. [online]. 2012, vol.23, n.3, pp. 87-94. ISSN 0718-0764. http://dx.doi.org/10.4067/S0718-07642012000300011.
The aim of this study was isolating and selecting wild bacteriophages from waters of the Lujan river in Argentina, against Salmonella Enteritidis, phage typing 86 strains of this serovariety and evaluating the genetic profiles of samples from the emerged specific groups. Bacteriophages concentration of river water was performed using the Moore swab with 60 minutes of exposure to water flow. Isolation and purification was carried out by the technique of double-layer agar. Eighty six isolates were phage-typed by standardized methodology and four groups of specificity appeared. Subtyping by pulsed field gel electrophoresis conducted on one sample of each group, establishing that two phage group corresponded to PT4 phagotype, which is the most common in Latin America. The other two groups had two genetic profiles that were not previously found in the database Latin America and the Caribbean PulseNet Network
Palavras-chave : genetic profiles; Salmonella Enteritidis; wild bacteriophages; phage typing.