versión On-line ISSN 0718-0764
BENAVIDES, Olga L; ARANGO, Oscar; HURTADO, Andrés M y ROJAS, Myriam C. Sapogenins Quantification of Fresh and Fermented Juice of Fique (Furcraea gigantean) by High-Performance Liquid Chromatography (HPLC-PDA). Inf. tecnol. [online]. 2012, vol.23, n.3, pp. 67-76. ISSN 0718-0764. http://dx.doi.org/10.4067/S0718-07642012000300009.
A stody on the quantification of sapogenins in fresh and fermented juice of fique (Forcraea gigantean) by high-performance liquid chromatography with photodiode array detector (HPLC-PDA). The juice of the fique, a plant that is widely grown in Colombia, was fermented for 2, 4 and 6 days at 33 °C. Using acid hydrolysis, sapogenin extracts were obtained. The sobstances in the extracts, identified by gas chromatography-mass spectrometry (GC-MS) were hecogenin and tigogenin. Hecogenin and tigogenin in the juice were determined osing HPLC-PDA. Fique juice with fuor days of fermentation showed the highest content of sapogenins. Therefore, this is the recommend process for the extraction of these compuonds.
Palabras clave : sapogenins; hecogenin; tigogenin; HPLC-PDA; GC-MS; Furcraea gigantea.