versión On-line ISSN 0718-0764
HERNANDEZ, Mónica; GALAN, Carlos A; ALVAREZ, Giaan A y PAEZ, M. E.. Development of an Amperometric Biosensor in Planar Configuration for the Quantification of Cholesterol . Inf. tecnol. [online]. 2011, vol.22, n.6, pp. 25-32. ISSN 0718-0764. http://dx.doi.org/10.4067/S0718-07642011000600004.
The development of a cholesterol amperometric biosensor in planar configuration is presented. The device is constructed by screen printing processes that are compatible with automated production methods. The electrochemical transducer incorporates tetracyanoquinodimethane, TCNQ, as mediator in order to reduce the work potential and minimize interferences. The biosensor presents an interval of lineal answer of 2- 12 mM with a limit of detection of 1.56 mM , requiring a volume of sample of only 7.2 µL. Its characteristics make it adequate for the analysis of cholesterol in blood serum and foods. The designed cholesterol amperometric biosensor demonstrates that without major technical or economic resources it is possible to develop biosensors with response characteristics competitive and compatible with mass production.
Palabras llave : amperometric biosensor; cholesterol; TCNQ; screen printing; mediator.