On-line version ISSN 0718-0764
MONTANEZ, José L; RAMOS, Emma G; ALEGRET, Salvador and DELGADO, Raúl J. Glucose Biosensor based on a Graphite-Epoxy-Platimum- Glucose Oxidase dispersed Biocomposite. Inf. tecnol. [online]. 2011, vol.22, n.1, pp. 29-40. ISSN 0718-0764. doi: 10.4067/S0718-07642011000100005.
The aim of this work was to develop a glucose biosensor based on electrochemical properties of a graphite-epoxy-platinum- glucose oxidase composite. The food and beverage industry demand fast, precise and reliable analytical methods to evaluate and to assure the quality of its products and to optimize its processes. The development of amperometric enzyme biosensors represents a viable option that satisfies these requirements. The working potential and the characterization of the response of the biosensor were determined by cyclic voltammetry and amperometry, respectively. The biosensor response was máximum at 600 mV, with a response time of 20 seconds within the concentration range of linear response from 0.1 to 5 mM glucose and sensitivity of 1.4 μA/mM. The stability and lifetime of the proposed biosensor depend on the frequency of use, where the biocomposite acts as a reservoir of enzymes and electrochemical mediator.
Keywords : analytical method; biosensor; glucose; platinum; voltammetry.