versión On-line ISSN 0718-0764
VAZQUEZ, Imir R; AGUILERA, Antonio F; PRADO-BARRAGAN, Lilia A y AGUILAR, Cristóbal N. Fungal Production of Proteases Induced with Pig Hair. Inf. tecnol. [online]. 2008, vol.19, n.2, pp.33-40. ISSN 0718-0764. http://dx.doi.org/10.4067/S0718-07642008000200005.
The production of new proteases induced with pig hair was evaluated using two systems, submerged culture and solid state culture. Culture medium was Czapek-Dox for both systems. Enzyme production kinetic was monitored each 24 hours during 120 hours. Protelytic activity was evaluated with the azocaseín method and biomass production was determined by the gravimetric method. Rhizopus oryzae was the microorganism with the capacity of using the pig hair as the single carbon-nitrogen source, producing in submerged fermentation the highest enzymatic titers. The effect of pH, the substrate pretreatment and addition of different levels of substrate on proteolyitic activity and biomass production were determined. Results permitted to obtain titers of proteolytic activity of 975 U/L.
Palabras clave : protease; pig hair; Rhizopus oryzae; fungal production.