Journal of the Chilean Chemical Society
versión On-line ISSN 0717-9707
KHANMOHAMMADI, MOHAMMADREZA; SOLEIMANI, MAJID; AFSHAR, MAJID y BAGHERI GARMARUDI, AMIR. PARTIAL LEAST SQUARES FOR SIMULTANEOUS DETERMINATION OF FE (III) AND HG(II) IN WATER AND PHARMACEUTICAL PREPARATIONS BY RP-HPLC USING 1,4-BIS-(4-PYRIDIL)-2,3-DIAZO-1,3-BUTADIENE CHELATING AGENT. J. Chil. Chem. Soc. [online]. 2011, vol.56, n.3, pp.774-777. ISSN 0717-9707. http://dx.doi.org/10.4067/S0717-97072011000300012.
A method has been developed for simultaneous determination of Fe(III) and Hg(II) by RP-HPLC, utilizing partial least squares technique. Vertex C18, with UV-visible detector was applied in reversed phase high performance liquid chromatography, while 1,4-bis-(4-Pyridil)-2,3-diazo-1,3-butadiene (4-bpdb) was added to the mobile phase as the chelating agent. Several effective parameters e.g. mobile phase make up, buffers, concentration of 4-bpdp and pH salts were investigated in order to optimize the quantification conditions. The optimum condition was achieved at 25°C, 10% methanol containing mobile phase, 30 mmol L1 acetate buffer, 0.05 mmol L1 4-bpdb and pH=4.5. Partial least squares chemometric technique was applied. The root mean square error (RMSE) of independent test set in the optimized model was 0.024 and 0.393 for Fe(III) and Hg(II) respectively.
Palabras clave : Determination; Complexation; 1,4-bis-(4-Pyridil)-2,3-diazo-1,3-butadiene; Mercury; Iron ; RP-HPLC, PLS.