Journal of the Chilean Chemical Society
versión ISSN 0717-9707
BRAVO, FELIPE; LOBOS, CLAUDIO; VENEGAS, KARINA y BENITES, JULIO. DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA. J. Chil. Chem. Soc. [online]. 2010, vol.55, n.4, pp. 454-457. ISSN 0717-9707. doi: 10.4067/S0717-97072010000400009.
Four benzodiazepines flunitrazepam, nitrazepam, clonazepam, and alprazolam, have been analyzed from biological matrices by dual column gas chromatography using a nitrogen-phosphorus detector (NPD) and a micro-electron capture detector (m-ECD). The recoveries of the four benzodiazepines spiked into human whole blood and plasma were 88.8 - 97.9 and 90.7 - 97.5%, respectively. The regression equations for the four benzodiazepines showed excellent linearity. The detection limits (LODs) were 0.14 - 0.95 ng/mL for whole blood and 0.13 - 0.93 ng/mL for plasma. The method is simple and sensitive for the determination of benzodiazepines in human whole blood and plasma, and may be useful in forensic science practice.
Palabras clave : Benzodiazepines; gas chromatography; nitrogen-phosphorus detector; micro-electron capture detector.