Journal of the Chilean Chemical Society
versión On-line ISSN 0717-9707
SANCHEZ, C. O et al. BIOSENSORS' PREPARATION TO BE USED FOR ENTRAPMENT METHOD WITH COPOLYMERS POLYANILINE DERIVATIVES. J. Chil. Chem. Soc. [online]. 2010, vol.55, n.2, pp. 233-235. ISSN 0717-9707. http://dx.doi.org/10.4067/S0717-97072010000200019.
The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at pH 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H2O2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of currentresponse, limitedbythe cyclenumbers of the cyclic voltammetry, 232 s, andenough stability to allow for the measurements to be taken after several days.
Palabras llave : Copolymers; Electrochemistry; enzyme; biosensor.