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Journal of the Chilean Chemical Society

versión On-line ISSN 0717-9707

Resumen

SULTANA, NAJMA et al. A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF DILTIAZEM AND QUINOLONES IN BULK, FORMULATIONS AND HUMAN SERUM. J. Chil. Chem. Soc. [online]. 2009, vol.54, n.4, pp. 358-362. ISSN 0717-9707.  http://dx.doi.org/10.4067/S0717-97072009000400007.

High Performance Liquid Chromatographic method was developed and applied for the simultaneous determination of diltiazem and fluoroquinolones in bulk, pharmaceutical formulations and human serum in presence of caffeine as internal standard. A Hiber®, RP-18 column was used with mobile phase consisting of acetonitrile:methanol:water (30:20:50 v/v, pH 3.6) and quantitative evaluation was performed at 230 run. Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with the requirements laid down by International Conference on Harmonization (ICH) guidelines.The method was selective, precise, accurate and can be used for routine analysis of pharmaceutical preparations, quality control and clinical laboratories.

Palabras clave : Diltiazem; floroquinolones; method validation; HPLC determination.

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