Journal of the Chilean Chemical Society
versión On-line ISSN 0717-9707
BCER, ENDER y QETNKAYA, PAKZE. A VOLTAMMETRIC STUDY ON THE INTERACTION OF NOVOBIOCIN WITH CYSTEINE. J. Chil. Chem. Soc. [online]. 2009, vol.54, n.1, pp.46-50. ISSN 0717-9707. http://dx.doi.org/10.4067/S0717-97072009000100011.
The interaction of novobiocin (NOV), an aminocoumarin antibiotic, with cysteine was studied by square-wave voltammetry technique on the hanging mercury drop electrode in different pH values. After the addition of NOV into the cysteine solution, the peak current of mercurous cysteine thiolate decreased and its voltammetric peak potential shifted to more positive values. Voltammetric results showed that NOV binds with cysteine forming 1:1 nonelectroactive molecular complex by means of electrostatic and hydrogen-bonding interactions. The binding constants of NOV with cysteine at pHs 5, 7 and 10 were calculated to be 3.06x103, 1.54x104 and 1.06x105 M-1, respectively. Furthermore, apossible mechanism of such interaction was also discussed.
Palabras clave : Voltammetry; Novobiocin; Cysteine; Interaction; pH effect.